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Combined bisdemethoxycurcumin and potassium iodide-mediated antimicrobial photodynamic therapy
Antimicrobial photodynamic therapy is emerging as a promising way to treat infections with minimal side effects. Typically, a single photosensitizer used in photodynamic therapy is capable of generating only one type of reactive oxygen species, which may have inadequate capability to eradicate certa...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10345248/ https://www.ncbi.nlm.nih.gov/pubmed/37455953 http://dx.doi.org/10.1016/j.heliyon.2023.e17490 |
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author | Damrongrungruang, Teerasak Panutyothin, Nichapat Kongjun, Sirapakorn Thanabat, Kittapak Ratha, Juthamat |
author_facet | Damrongrungruang, Teerasak Panutyothin, Nichapat Kongjun, Sirapakorn Thanabat, Kittapak Ratha, Juthamat |
author_sort | Damrongrungruang, Teerasak |
collection | PubMed |
description | Antimicrobial photodynamic therapy is emerging as a promising way to treat infections with minimal side effects. Typically, a single photosensitizer used in photodynamic therapy is capable of generating only one type of reactive oxygen species, which may have inadequate capability to eradicate certain types of microbes, especially Candida species. Thus, the use of combined photosensitizers is examined as a means of achieving superior antimicrobial results. We postulate that bisdemethoxycurcumin, a type I reactive oxygen species generator, combined with potassium iodide, an antimicrobial iodide molecule, might exhibit superior antimicrobial effects compared to a single photosensitizer-mediated photodynamic therapy. The effects of bisdemethoxycurcumin + potassium iodide + dental blue light on Candida albicans reduction were examined. Candida biofilms were treated with 20, 40 or 80 μM bisdemethoxycurcumin, 100 mM potassium iodide or a combination of these species for 20 min before irradiation with a dental blue light (90 J/cm(2)). The negative and positive controls were phosphate buffer saline and nystatin at 1 : 100,000 units/ml, respectively. Candidal numbers were quantified at 0, 1, 6 and 24 h. Hydroxyl radicals were spectrophotometrically measured using 2-[6-(4′amino phynoxyl-3H-xanthen-3-on-9-yl)] benzoic acid or APF probe-mediated fluorescence intensity (Varioskan) at 490/515 nm (excitation/emission). Candidal counts and hydroxyl radical comparisons were performed using the Kruskal-Wallis test and one-way ANOVA, respectively. Correlations between candidal numbers and hydroxyl radical levels were done with a Pearson correlation test. Forty μM bisdemethoxycurcumin+100 mM KI could provide a 3.5 log(10) CFU/ml reduction after 6 h. Bisdemethoxycurcumin alone generated OH levels that were strongly correlated with candidal reduction. In conclusion, 40 μM bisdemethoxycurcumin+100 mM KI could reduce C. albicans biofilm. |
format | Online Article Text |
id | pubmed-10345248 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-103452482023-07-15 Combined bisdemethoxycurcumin and potassium iodide-mediated antimicrobial photodynamic therapy Damrongrungruang, Teerasak Panutyothin, Nichapat Kongjun, Sirapakorn Thanabat, Kittapak Ratha, Juthamat Heliyon Research Article Antimicrobial photodynamic therapy is emerging as a promising way to treat infections with minimal side effects. Typically, a single photosensitizer used in photodynamic therapy is capable of generating only one type of reactive oxygen species, which may have inadequate capability to eradicate certain types of microbes, especially Candida species. Thus, the use of combined photosensitizers is examined as a means of achieving superior antimicrobial results. We postulate that bisdemethoxycurcumin, a type I reactive oxygen species generator, combined with potassium iodide, an antimicrobial iodide molecule, might exhibit superior antimicrobial effects compared to a single photosensitizer-mediated photodynamic therapy. The effects of bisdemethoxycurcumin + potassium iodide + dental blue light on Candida albicans reduction were examined. Candida biofilms were treated with 20, 40 or 80 μM bisdemethoxycurcumin, 100 mM potassium iodide or a combination of these species for 20 min before irradiation with a dental blue light (90 J/cm(2)). The negative and positive controls were phosphate buffer saline and nystatin at 1 : 100,000 units/ml, respectively. Candidal numbers were quantified at 0, 1, 6 and 24 h. Hydroxyl radicals were spectrophotometrically measured using 2-[6-(4′amino phynoxyl-3H-xanthen-3-on-9-yl)] benzoic acid or APF probe-mediated fluorescence intensity (Varioskan) at 490/515 nm (excitation/emission). Candidal counts and hydroxyl radical comparisons were performed using the Kruskal-Wallis test and one-way ANOVA, respectively. Correlations between candidal numbers and hydroxyl radical levels were done with a Pearson correlation test. Forty μM bisdemethoxycurcumin+100 mM KI could provide a 3.5 log(10) CFU/ml reduction after 6 h. Bisdemethoxycurcumin alone generated OH levels that were strongly correlated with candidal reduction. In conclusion, 40 μM bisdemethoxycurcumin+100 mM KI could reduce C. albicans biofilm. Elsevier 2023-06-27 /pmc/articles/PMC10345248/ /pubmed/37455953 http://dx.doi.org/10.1016/j.heliyon.2023.e17490 Text en © 2023 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Research Article Damrongrungruang, Teerasak Panutyothin, Nichapat Kongjun, Sirapakorn Thanabat, Kittapak Ratha, Juthamat Combined bisdemethoxycurcumin and potassium iodide-mediated antimicrobial photodynamic therapy |
title | Combined bisdemethoxycurcumin and potassium iodide-mediated antimicrobial photodynamic therapy |
title_full | Combined bisdemethoxycurcumin and potassium iodide-mediated antimicrobial photodynamic therapy |
title_fullStr | Combined bisdemethoxycurcumin and potassium iodide-mediated antimicrobial photodynamic therapy |
title_full_unstemmed | Combined bisdemethoxycurcumin and potassium iodide-mediated antimicrobial photodynamic therapy |
title_short | Combined bisdemethoxycurcumin and potassium iodide-mediated antimicrobial photodynamic therapy |
title_sort | combined bisdemethoxycurcumin and potassium iodide-mediated antimicrobial photodynamic therapy |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10345248/ https://www.ncbi.nlm.nih.gov/pubmed/37455953 http://dx.doi.org/10.1016/j.heliyon.2023.e17490 |
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