Cargando…

Protective effect of mussel polysaccharide on cyclophosphamide‐induced intestinal oxidative stress injury via Nrf2‐Keap1 signaling pathway

The hard‐shelled mussel (Mytilus coruscus) has been used as a traditional Chinese medicine and health food in China for centuries. Polysaccharides from mussel has been reported to have multiple biological functions, however, it remains unclear whether mussel polysaccharide (MP) exerts protective eff...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhao, Zhen‐Lei, Xu, Xiao‐Gang, Chang, Yun‐Chuang, Xu, Yi‐Peng, Zhou, Xu‐Qiang, Su, Hui‐Li, Cui, Xiao‐Hua, Wan, Xiao‐Qing, Mao, Gen‐Xiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10345665/
https://www.ncbi.nlm.nih.gov/pubmed/37457170
http://dx.doi.org/10.1002/fsn3.3453
_version_ 1785073141079343104
author Zhao, Zhen‐Lei
Xu, Xiao‐Gang
Chang, Yun‐Chuang
Xu, Yi‐Peng
Zhou, Xu‐Qiang
Su, Hui‐Li
Cui, Xiao‐Hua
Wan, Xiao‐Qing
Mao, Gen‐Xiang
author_facet Zhao, Zhen‐Lei
Xu, Xiao‐Gang
Chang, Yun‐Chuang
Xu, Yi‐Peng
Zhou, Xu‐Qiang
Su, Hui‐Li
Cui, Xiao‐Hua
Wan, Xiao‐Qing
Mao, Gen‐Xiang
author_sort Zhao, Zhen‐Lei
collection PubMed
description The hard‐shelled mussel (Mytilus coruscus) has been used as a traditional Chinese medicine and health food in China for centuries. Polysaccharides from mussel has been reported to have multiple biological functions, however, it remains unclear whether mussel polysaccharide (MP) exerts protective effects in intestinal functions, and the underlying mechanisms of action remain unclear. The aim of this study was to investigate the protective effects and mechanism of MP on intestinal oxidative injury in mice. In this study, 40 male BALB/C mice were used, with 30 utilized to produce an animal model of intestinal oxidative injury with intraperitoneal injection of cyclophosphamide (Cy) for four consecutive days. The protective effects of two different doses of MP (300 and 600 mg/kg) were assessed by investigating the change in body weight, visceral index, and observing colon histomorphology. Moreover, the underlying molecular mechanisms were investigated by measuring the antioxidant enzymes and related signaling molecules through ELISA, real‐time PCR, and western blot methods. The results showed that MP pretreatment effectively protected the intestinal from Cy‐induced injury: improved the colon tissue morphology and villus structure, increased superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH‐Px) activities, and reduced malondialdehyde (MDA) content in serum and colon tissues. Meanwhile, MP also significantly increased the expression levels of SOD, GSH‐Px, heme oxygenase‐1 (HO‐1), and nuclear factor E2‐related factor 2 (Nrf2) mRNA in colon tissues. Further, western blot results showed that the expression of Nrf2 protein was significantly upregulated while kelch‐like ECH‐associated protein 1 (Keap1) was significantly downregulated by MP in the colonic tissues. This study indicates that MP can ameliorate Cy‐induced oxidative stress injury in mice, and Nrf2‐Keap1 signaling pathway may mediate these protective effects.
format Online
Article
Text
id pubmed-10345665
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher John Wiley and Sons Inc.
record_format MEDLINE/PubMed
spelling pubmed-103456652023-07-15 Protective effect of mussel polysaccharide on cyclophosphamide‐induced intestinal oxidative stress injury via Nrf2‐Keap1 signaling pathway Zhao, Zhen‐Lei Xu, Xiao‐Gang Chang, Yun‐Chuang Xu, Yi‐Peng Zhou, Xu‐Qiang Su, Hui‐Li Cui, Xiao‐Hua Wan, Xiao‐Qing Mao, Gen‐Xiang Food Sci Nutr Original Articles The hard‐shelled mussel (Mytilus coruscus) has been used as a traditional Chinese medicine and health food in China for centuries. Polysaccharides from mussel has been reported to have multiple biological functions, however, it remains unclear whether mussel polysaccharide (MP) exerts protective effects in intestinal functions, and the underlying mechanisms of action remain unclear. The aim of this study was to investigate the protective effects and mechanism of MP on intestinal oxidative injury in mice. In this study, 40 male BALB/C mice were used, with 30 utilized to produce an animal model of intestinal oxidative injury with intraperitoneal injection of cyclophosphamide (Cy) for four consecutive days. The protective effects of two different doses of MP (300 and 600 mg/kg) were assessed by investigating the change in body weight, visceral index, and observing colon histomorphology. Moreover, the underlying molecular mechanisms were investigated by measuring the antioxidant enzymes and related signaling molecules through ELISA, real‐time PCR, and western blot methods. The results showed that MP pretreatment effectively protected the intestinal from Cy‐induced injury: improved the colon tissue morphology and villus structure, increased superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH‐Px) activities, and reduced malondialdehyde (MDA) content in serum and colon tissues. Meanwhile, MP also significantly increased the expression levels of SOD, GSH‐Px, heme oxygenase‐1 (HO‐1), and nuclear factor E2‐related factor 2 (Nrf2) mRNA in colon tissues. Further, western blot results showed that the expression of Nrf2 protein was significantly upregulated while kelch‐like ECH‐associated protein 1 (Keap1) was significantly downregulated by MP in the colonic tissues. This study indicates that MP can ameliorate Cy‐induced oxidative stress injury in mice, and Nrf2‐Keap1 signaling pathway may mediate these protective effects. John Wiley and Sons Inc. 2023-05-23 /pmc/articles/PMC10345665/ /pubmed/37457170 http://dx.doi.org/10.1002/fsn3.3453 Text en © 2023 The Authors. Food Science & Nutrition published by Wiley Periodicals LLC. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Zhao, Zhen‐Lei
Xu, Xiao‐Gang
Chang, Yun‐Chuang
Xu, Yi‐Peng
Zhou, Xu‐Qiang
Su, Hui‐Li
Cui, Xiao‐Hua
Wan, Xiao‐Qing
Mao, Gen‐Xiang
Protective effect of mussel polysaccharide on cyclophosphamide‐induced intestinal oxidative stress injury via Nrf2‐Keap1 signaling pathway
title Protective effect of mussel polysaccharide on cyclophosphamide‐induced intestinal oxidative stress injury via Nrf2‐Keap1 signaling pathway
title_full Protective effect of mussel polysaccharide on cyclophosphamide‐induced intestinal oxidative stress injury via Nrf2‐Keap1 signaling pathway
title_fullStr Protective effect of mussel polysaccharide on cyclophosphamide‐induced intestinal oxidative stress injury via Nrf2‐Keap1 signaling pathway
title_full_unstemmed Protective effect of mussel polysaccharide on cyclophosphamide‐induced intestinal oxidative stress injury via Nrf2‐Keap1 signaling pathway
title_short Protective effect of mussel polysaccharide on cyclophosphamide‐induced intestinal oxidative stress injury via Nrf2‐Keap1 signaling pathway
title_sort protective effect of mussel polysaccharide on cyclophosphamide‐induced intestinal oxidative stress injury via nrf2‐keap1 signaling pathway
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10345665/
https://www.ncbi.nlm.nih.gov/pubmed/37457170
http://dx.doi.org/10.1002/fsn3.3453
work_keys_str_mv AT zhaozhenlei protectiveeffectofmusselpolysaccharideoncyclophosphamideinducedintestinaloxidativestressinjuryvianrf2keap1signalingpathway
AT xuxiaogang protectiveeffectofmusselpolysaccharideoncyclophosphamideinducedintestinaloxidativestressinjuryvianrf2keap1signalingpathway
AT changyunchuang protectiveeffectofmusselpolysaccharideoncyclophosphamideinducedintestinaloxidativestressinjuryvianrf2keap1signalingpathway
AT xuyipeng protectiveeffectofmusselpolysaccharideoncyclophosphamideinducedintestinaloxidativestressinjuryvianrf2keap1signalingpathway
AT zhouxuqiang protectiveeffectofmusselpolysaccharideoncyclophosphamideinducedintestinaloxidativestressinjuryvianrf2keap1signalingpathway
AT suhuili protectiveeffectofmusselpolysaccharideoncyclophosphamideinducedintestinaloxidativestressinjuryvianrf2keap1signalingpathway
AT cuixiaohua protectiveeffectofmusselpolysaccharideoncyclophosphamideinducedintestinaloxidativestressinjuryvianrf2keap1signalingpathway
AT wanxiaoqing protectiveeffectofmusselpolysaccharideoncyclophosphamideinducedintestinaloxidativestressinjuryvianrf2keap1signalingpathway
AT maogenxiang protectiveeffectofmusselpolysaccharideoncyclophosphamideinducedintestinaloxidativestressinjuryvianrf2keap1signalingpathway