Cargando…
Chamaecyparis lawsoniana Leaf Essential Oil as a Potential Anticancer Agent: Experimental and Computational Studies
Cancer remains one of the leading causes of death worldwide, affected by several factors including oxidative stress; and although conventional synthetic medicines have been used to treat cancer, they often result in various side effects. Consequently, there is a growing need for newer, safer and mor...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10346282/ https://www.ncbi.nlm.nih.gov/pubmed/37447036 http://dx.doi.org/10.3390/plants12132475 |
_version_ | 1785073278460624896 |
---|---|
author | Fikry, Eman Orfali, Raha Elbaramawi, Samar S. Perveen, Shagufta El-Shafae, Azza M. El-Domiaty, Maher M. Tawfeek, Nora |
author_facet | Fikry, Eman Orfali, Raha Elbaramawi, Samar S. Perveen, Shagufta El-Shafae, Azza M. El-Domiaty, Maher M. Tawfeek, Nora |
author_sort | Fikry, Eman |
collection | PubMed |
description | Cancer remains one of the leading causes of death worldwide, affected by several factors including oxidative stress; and although conventional synthetic medicines have been used to treat cancer, they often result in various side effects. Consequently, there is a growing need for newer, safer and more effective alternatives, such as natural plant products. Essential oils (EOs) are one such alternative, offering a wide range of bioactivities, including antibacterial, antiviral, antioxidant, and anticancer properties. Accordingly, the objective of the present study was to investigate the chemical composition, as well as the antioxidant and anticancer properties of the leaf essential oil of Chamaecyparis lawsoniana (CLLEO) belonging to the Cupressaceae family. Totally, 59 constituents were identified by gas chromatography-mass spectrometry (GC-MS) analysis. cis-Abienol, trans-ferruginol, α-cadinol, δ-muurolene and α-pinene were the major components. The in vitro cytotoxicity study against human breast (MCF-7), colon (HCT-116), lung (A-549), hepatocellular (HepG-2) carcinoma cells using MTT assay indicated a promising cytotoxic activity against all the tested cancer cells, particularly HepG-2, with significant selectivity indices. CLLEO exhibited weak antioxidant activity according to the DPPH, ABTS and FRAP assays. In silico docking of these constituents against the epidermal growth factor receptor (EGFR), the myeloid cell leukemia-1 (Mcl-1) and caspase-8 using Molecular Operating Environment (MOE) software demonstrated good binding affinities of the components with the active site of these targets. These findings suggested using CLLEO, or its individual components, as a potentially viable therapeutic option for managing cancerous conditions. |
format | Online Article Text |
id | pubmed-10346282 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-103462822023-07-15 Chamaecyparis lawsoniana Leaf Essential Oil as a Potential Anticancer Agent: Experimental and Computational Studies Fikry, Eman Orfali, Raha Elbaramawi, Samar S. Perveen, Shagufta El-Shafae, Azza M. El-Domiaty, Maher M. Tawfeek, Nora Plants (Basel) Article Cancer remains one of the leading causes of death worldwide, affected by several factors including oxidative stress; and although conventional synthetic medicines have been used to treat cancer, they often result in various side effects. Consequently, there is a growing need for newer, safer and more effective alternatives, such as natural plant products. Essential oils (EOs) are one such alternative, offering a wide range of bioactivities, including antibacterial, antiviral, antioxidant, and anticancer properties. Accordingly, the objective of the present study was to investigate the chemical composition, as well as the antioxidant and anticancer properties of the leaf essential oil of Chamaecyparis lawsoniana (CLLEO) belonging to the Cupressaceae family. Totally, 59 constituents were identified by gas chromatography-mass spectrometry (GC-MS) analysis. cis-Abienol, trans-ferruginol, α-cadinol, δ-muurolene and α-pinene were the major components. The in vitro cytotoxicity study against human breast (MCF-7), colon (HCT-116), lung (A-549), hepatocellular (HepG-2) carcinoma cells using MTT assay indicated a promising cytotoxic activity against all the tested cancer cells, particularly HepG-2, with significant selectivity indices. CLLEO exhibited weak antioxidant activity according to the DPPH, ABTS and FRAP assays. In silico docking of these constituents against the epidermal growth factor receptor (EGFR), the myeloid cell leukemia-1 (Mcl-1) and caspase-8 using Molecular Operating Environment (MOE) software demonstrated good binding affinities of the components with the active site of these targets. These findings suggested using CLLEO, or its individual components, as a potentially viable therapeutic option for managing cancerous conditions. MDPI 2023-06-28 /pmc/articles/PMC10346282/ /pubmed/37447036 http://dx.doi.org/10.3390/plants12132475 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Fikry, Eman Orfali, Raha Elbaramawi, Samar S. Perveen, Shagufta El-Shafae, Azza M. El-Domiaty, Maher M. Tawfeek, Nora Chamaecyparis lawsoniana Leaf Essential Oil as a Potential Anticancer Agent: Experimental and Computational Studies |
title | Chamaecyparis lawsoniana Leaf Essential Oil as a Potential Anticancer Agent: Experimental and Computational Studies |
title_full | Chamaecyparis lawsoniana Leaf Essential Oil as a Potential Anticancer Agent: Experimental and Computational Studies |
title_fullStr | Chamaecyparis lawsoniana Leaf Essential Oil as a Potential Anticancer Agent: Experimental and Computational Studies |
title_full_unstemmed | Chamaecyparis lawsoniana Leaf Essential Oil as a Potential Anticancer Agent: Experimental and Computational Studies |
title_short | Chamaecyparis lawsoniana Leaf Essential Oil as a Potential Anticancer Agent: Experimental and Computational Studies |
title_sort | chamaecyparis lawsoniana leaf essential oil as a potential anticancer agent: experimental and computational studies |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10346282/ https://www.ncbi.nlm.nih.gov/pubmed/37447036 http://dx.doi.org/10.3390/plants12132475 |
work_keys_str_mv | AT fikryeman chamaecyparislawsonianaleafessentialoilasapotentialanticanceragentexperimentalandcomputationalstudies AT orfaliraha chamaecyparislawsonianaleafessentialoilasapotentialanticanceragentexperimentalandcomputationalstudies AT elbaramawisamars chamaecyparislawsonianaleafessentialoilasapotentialanticanceragentexperimentalandcomputationalstudies AT perveenshagufta chamaecyparislawsonianaleafessentialoilasapotentialanticanceragentexperimentalandcomputationalstudies AT elshafaeazzam chamaecyparislawsonianaleafessentialoilasapotentialanticanceragentexperimentalandcomputationalstudies AT eldomiatymaherm chamaecyparislawsonianaleafessentialoilasapotentialanticanceragentexperimentalandcomputationalstudies AT tawfeeknora chamaecyparislawsonianaleafessentialoilasapotentialanticanceragentexperimentalandcomputationalstudies |