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Carvacrol Improves Vascular Function in Hypertensive Animals by Modulating Endothelial Progenitor Cells

Carvacrol, a phenolic monoterpene, has diverse biological activities, highlighting its antioxidant and antihypertensive capacity. However, there is little evidence demonstrating its influence on vascular regeneration. Therefore, we evaluated the modulation of carvacrol on endothelial repair induced...

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Autores principales: Gonçalves, Tays Amanda Felisberto, Lima, Viviane Silva, de Almeida, Arthur José Pontes Oliveira, de Arruda, Alinne Villar, Veras, Ana Caroline Meneses Ferreira, Lima, Thaís Trajano, Soares, Evyllen Myllena Cardoso, dos Santos, Adhonias Correia, de Vasconcelos, Maria Eduarda Costa, de Almeida Feitosa, Mathania Silva, Veras, Robson Cavalcante, de Medeiros, Isac Almeida
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10346284/
https://www.ncbi.nlm.nih.gov/pubmed/37447358
http://dx.doi.org/10.3390/nu15133032
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author Gonçalves, Tays Amanda Felisberto
Lima, Viviane Silva
de Almeida, Arthur José Pontes Oliveira
de Arruda, Alinne Villar
Veras, Ana Caroline Meneses Ferreira
Lima, Thaís Trajano
Soares, Evyllen Myllena Cardoso
dos Santos, Adhonias Correia
de Vasconcelos, Maria Eduarda Costa
de Almeida Feitosa, Mathania Silva
Veras, Robson Cavalcante
de Medeiros, Isac Almeida
author_facet Gonçalves, Tays Amanda Felisberto
Lima, Viviane Silva
de Almeida, Arthur José Pontes Oliveira
de Arruda, Alinne Villar
Veras, Ana Caroline Meneses Ferreira
Lima, Thaís Trajano
Soares, Evyllen Myllena Cardoso
dos Santos, Adhonias Correia
de Vasconcelos, Maria Eduarda Costa
de Almeida Feitosa, Mathania Silva
Veras, Robson Cavalcante
de Medeiros, Isac Almeida
author_sort Gonçalves, Tays Amanda Felisberto
collection PubMed
description Carvacrol, a phenolic monoterpene, has diverse biological activities, highlighting its antioxidant and antihypertensive capacity. However, there is little evidence demonstrating its influence on vascular regeneration. Therefore, we evaluated the modulation of carvacrol on endothelial repair induced by endothelial progenitor cells (EPC) in hypertension. Twelve-week-old spontaneously hypertensive rats (SHR) were treated with a vehicle, carvacrol (50 or 100 mg/kg/day), or resveratrol (10 mg/kg/day) orally for four weeks. Wistar Kyoto (WKY) rats were used as the normotensive controls. Their systolic blood pressure (SBP) was measured weekly through the tail cuff. The EPCs were isolated from the bone marrow and peripherical circulation and were quantified by flow cytometry. The functionality of the EPC was evaluated after cultivation through the quantification of colony-forming units (CFU), evaluation of eNOS, intracellular detection of reactive oxygen species (ROS), and evaluation of senescence. The superior mesenteric artery was isolated to evaluate the quantification of ROS, CD34, and CD31. Treatment with carvacrol induced EPC migration, increased CFU formation and eNOS expression and activity, and reduced ROS and senescence. In addition, carvacrol reduced vascular ROS and increased CD31 and CD34 expression. This study showed that treatment with carvacrol improved the functionality of EPC, contributing to the reduction of endothelial dysfunction.
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spelling pubmed-103462842023-07-15 Carvacrol Improves Vascular Function in Hypertensive Animals by Modulating Endothelial Progenitor Cells Gonçalves, Tays Amanda Felisberto Lima, Viviane Silva de Almeida, Arthur José Pontes Oliveira de Arruda, Alinne Villar Veras, Ana Caroline Meneses Ferreira Lima, Thaís Trajano Soares, Evyllen Myllena Cardoso dos Santos, Adhonias Correia de Vasconcelos, Maria Eduarda Costa de Almeida Feitosa, Mathania Silva Veras, Robson Cavalcante de Medeiros, Isac Almeida Nutrients Article Carvacrol, a phenolic monoterpene, has diverse biological activities, highlighting its antioxidant and antihypertensive capacity. However, there is little evidence demonstrating its influence on vascular regeneration. Therefore, we evaluated the modulation of carvacrol on endothelial repair induced by endothelial progenitor cells (EPC) in hypertension. Twelve-week-old spontaneously hypertensive rats (SHR) were treated with a vehicle, carvacrol (50 or 100 mg/kg/day), or resveratrol (10 mg/kg/day) orally for four weeks. Wistar Kyoto (WKY) rats were used as the normotensive controls. Their systolic blood pressure (SBP) was measured weekly through the tail cuff. The EPCs were isolated from the bone marrow and peripherical circulation and were quantified by flow cytometry. The functionality of the EPC was evaluated after cultivation through the quantification of colony-forming units (CFU), evaluation of eNOS, intracellular detection of reactive oxygen species (ROS), and evaluation of senescence. The superior mesenteric artery was isolated to evaluate the quantification of ROS, CD34, and CD31. Treatment with carvacrol induced EPC migration, increased CFU formation and eNOS expression and activity, and reduced ROS and senescence. In addition, carvacrol reduced vascular ROS and increased CD31 and CD34 expression. This study showed that treatment with carvacrol improved the functionality of EPC, contributing to the reduction of endothelial dysfunction. MDPI 2023-07-04 /pmc/articles/PMC10346284/ /pubmed/37447358 http://dx.doi.org/10.3390/nu15133032 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gonçalves, Tays Amanda Felisberto
Lima, Viviane Silva
de Almeida, Arthur José Pontes Oliveira
de Arruda, Alinne Villar
Veras, Ana Caroline Meneses Ferreira
Lima, Thaís Trajano
Soares, Evyllen Myllena Cardoso
dos Santos, Adhonias Correia
de Vasconcelos, Maria Eduarda Costa
de Almeida Feitosa, Mathania Silva
Veras, Robson Cavalcante
de Medeiros, Isac Almeida
Carvacrol Improves Vascular Function in Hypertensive Animals by Modulating Endothelial Progenitor Cells
title Carvacrol Improves Vascular Function in Hypertensive Animals by Modulating Endothelial Progenitor Cells
title_full Carvacrol Improves Vascular Function in Hypertensive Animals by Modulating Endothelial Progenitor Cells
title_fullStr Carvacrol Improves Vascular Function in Hypertensive Animals by Modulating Endothelial Progenitor Cells
title_full_unstemmed Carvacrol Improves Vascular Function in Hypertensive Animals by Modulating Endothelial Progenitor Cells
title_short Carvacrol Improves Vascular Function in Hypertensive Animals by Modulating Endothelial Progenitor Cells
title_sort carvacrol improves vascular function in hypertensive animals by modulating endothelial progenitor cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10346284/
https://www.ncbi.nlm.nih.gov/pubmed/37447358
http://dx.doi.org/10.3390/nu15133032
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