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Carvacrol Improves Vascular Function in Hypertensive Animals by Modulating Endothelial Progenitor Cells
Carvacrol, a phenolic monoterpene, has diverse biological activities, highlighting its antioxidant and antihypertensive capacity. However, there is little evidence demonstrating its influence on vascular regeneration. Therefore, we evaluated the modulation of carvacrol on endothelial repair induced...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10346284/ https://www.ncbi.nlm.nih.gov/pubmed/37447358 http://dx.doi.org/10.3390/nu15133032 |
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author | Gonçalves, Tays Amanda Felisberto Lima, Viviane Silva de Almeida, Arthur José Pontes Oliveira de Arruda, Alinne Villar Veras, Ana Caroline Meneses Ferreira Lima, Thaís Trajano Soares, Evyllen Myllena Cardoso dos Santos, Adhonias Correia de Vasconcelos, Maria Eduarda Costa de Almeida Feitosa, Mathania Silva Veras, Robson Cavalcante de Medeiros, Isac Almeida |
author_facet | Gonçalves, Tays Amanda Felisberto Lima, Viviane Silva de Almeida, Arthur José Pontes Oliveira de Arruda, Alinne Villar Veras, Ana Caroline Meneses Ferreira Lima, Thaís Trajano Soares, Evyllen Myllena Cardoso dos Santos, Adhonias Correia de Vasconcelos, Maria Eduarda Costa de Almeida Feitosa, Mathania Silva Veras, Robson Cavalcante de Medeiros, Isac Almeida |
author_sort | Gonçalves, Tays Amanda Felisberto |
collection | PubMed |
description | Carvacrol, a phenolic monoterpene, has diverse biological activities, highlighting its antioxidant and antihypertensive capacity. However, there is little evidence demonstrating its influence on vascular regeneration. Therefore, we evaluated the modulation of carvacrol on endothelial repair induced by endothelial progenitor cells (EPC) in hypertension. Twelve-week-old spontaneously hypertensive rats (SHR) were treated with a vehicle, carvacrol (50 or 100 mg/kg/day), or resveratrol (10 mg/kg/day) orally for four weeks. Wistar Kyoto (WKY) rats were used as the normotensive controls. Their systolic blood pressure (SBP) was measured weekly through the tail cuff. The EPCs were isolated from the bone marrow and peripherical circulation and were quantified by flow cytometry. The functionality of the EPC was evaluated after cultivation through the quantification of colony-forming units (CFU), evaluation of eNOS, intracellular detection of reactive oxygen species (ROS), and evaluation of senescence. The superior mesenteric artery was isolated to evaluate the quantification of ROS, CD34, and CD31. Treatment with carvacrol induced EPC migration, increased CFU formation and eNOS expression and activity, and reduced ROS and senescence. In addition, carvacrol reduced vascular ROS and increased CD31 and CD34 expression. This study showed that treatment with carvacrol improved the functionality of EPC, contributing to the reduction of endothelial dysfunction. |
format | Online Article Text |
id | pubmed-10346284 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-103462842023-07-15 Carvacrol Improves Vascular Function in Hypertensive Animals by Modulating Endothelial Progenitor Cells Gonçalves, Tays Amanda Felisberto Lima, Viviane Silva de Almeida, Arthur José Pontes Oliveira de Arruda, Alinne Villar Veras, Ana Caroline Meneses Ferreira Lima, Thaís Trajano Soares, Evyllen Myllena Cardoso dos Santos, Adhonias Correia de Vasconcelos, Maria Eduarda Costa de Almeida Feitosa, Mathania Silva Veras, Robson Cavalcante de Medeiros, Isac Almeida Nutrients Article Carvacrol, a phenolic monoterpene, has diverse biological activities, highlighting its antioxidant and antihypertensive capacity. However, there is little evidence demonstrating its influence on vascular regeneration. Therefore, we evaluated the modulation of carvacrol on endothelial repair induced by endothelial progenitor cells (EPC) in hypertension. Twelve-week-old spontaneously hypertensive rats (SHR) were treated with a vehicle, carvacrol (50 or 100 mg/kg/day), or resveratrol (10 mg/kg/day) orally for four weeks. Wistar Kyoto (WKY) rats were used as the normotensive controls. Their systolic blood pressure (SBP) was measured weekly through the tail cuff. The EPCs were isolated from the bone marrow and peripherical circulation and were quantified by flow cytometry. The functionality of the EPC was evaluated after cultivation through the quantification of colony-forming units (CFU), evaluation of eNOS, intracellular detection of reactive oxygen species (ROS), and evaluation of senescence. The superior mesenteric artery was isolated to evaluate the quantification of ROS, CD34, and CD31. Treatment with carvacrol induced EPC migration, increased CFU formation and eNOS expression and activity, and reduced ROS and senescence. In addition, carvacrol reduced vascular ROS and increased CD31 and CD34 expression. This study showed that treatment with carvacrol improved the functionality of EPC, contributing to the reduction of endothelial dysfunction. MDPI 2023-07-04 /pmc/articles/PMC10346284/ /pubmed/37447358 http://dx.doi.org/10.3390/nu15133032 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Gonçalves, Tays Amanda Felisberto Lima, Viviane Silva de Almeida, Arthur José Pontes Oliveira de Arruda, Alinne Villar Veras, Ana Caroline Meneses Ferreira Lima, Thaís Trajano Soares, Evyllen Myllena Cardoso dos Santos, Adhonias Correia de Vasconcelos, Maria Eduarda Costa de Almeida Feitosa, Mathania Silva Veras, Robson Cavalcante de Medeiros, Isac Almeida Carvacrol Improves Vascular Function in Hypertensive Animals by Modulating Endothelial Progenitor Cells |
title | Carvacrol Improves Vascular Function in Hypertensive Animals by Modulating Endothelial Progenitor Cells |
title_full | Carvacrol Improves Vascular Function in Hypertensive Animals by Modulating Endothelial Progenitor Cells |
title_fullStr | Carvacrol Improves Vascular Function in Hypertensive Animals by Modulating Endothelial Progenitor Cells |
title_full_unstemmed | Carvacrol Improves Vascular Function in Hypertensive Animals by Modulating Endothelial Progenitor Cells |
title_short | Carvacrol Improves Vascular Function in Hypertensive Animals by Modulating Endothelial Progenitor Cells |
title_sort | carvacrol improves vascular function in hypertensive animals by modulating endothelial progenitor cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10346284/ https://www.ncbi.nlm.nih.gov/pubmed/37447358 http://dx.doi.org/10.3390/nu15133032 |
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