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2-Mercaptobenzimidazole Functionalized Copper Nanoparticles Fluorescence Probe for Sensitivity and Selectivity Detection of Cys in Serum

In this paper, a 2-mercaptobenzimidazole-copper nanoparticles (MBI-CuNPs) fluorescent probe with high performance based on 2-mercaptobenzimidazole functionalized copper nanoparticles was synthesized by a hydrothermal method and used for cysteine (Cys) detection in serum. The MBI-CuNPs probe exhibits...

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Detalles Bibliográficos
Autores principales: Liu, Jing, Dou, Xiaozong, Zhang, Hongyan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10346489/
https://www.ncbi.nlm.nih.gov/pubmed/37447664
http://dx.doi.org/10.3390/s23135814
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author Liu, Jing
Dou, Xiaozong
Zhang, Hongyan
author_facet Liu, Jing
Dou, Xiaozong
Zhang, Hongyan
author_sort Liu, Jing
collection PubMed
description In this paper, a 2-mercaptobenzimidazole-copper nanoparticles (MBI-CuNPs) fluorescent probe with high performance based on 2-mercaptobenzimidazole functionalized copper nanoparticles was synthesized by a hydrothermal method and used for cysteine (Cys) detection in serum. The MBI-CuNPs probe exhibits strong fluorescence emission at 415 nm under the excitation at 200 nm, which is attributed to the metal-ligand charge transfer (MLCT) transition through the coordination of an MBI ligand and monovalent copper. Furthermore, the MBI-CuNPs probe has a high quenching fluorescence response to Cys, and shows a good linearity relationship with Cys in 0.05–65 µM, with a detection limit of 52 nM. Moreover, the MBI-CuNPs probe could eliminate the interference of biological mercaptan Hcy and GSH with a similar structure and reaction properties, due to the strong electron-donating ability of Cys, which can quench the fluorescence of the MBI-CuNPs probe. The MBI-CuNPs probe was applied to the analysis of Cys in real serum, and the absolute recovery rate was as high as 90.23–97.00%. Such a fluorescent probe with high sensitivity and selectivity has potential applications for the early prevention of various diseases caused by abnormal Cys levels.
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spelling pubmed-103464892023-07-15 2-Mercaptobenzimidazole Functionalized Copper Nanoparticles Fluorescence Probe for Sensitivity and Selectivity Detection of Cys in Serum Liu, Jing Dou, Xiaozong Zhang, Hongyan Sensors (Basel) Article In this paper, a 2-mercaptobenzimidazole-copper nanoparticles (MBI-CuNPs) fluorescent probe with high performance based on 2-mercaptobenzimidazole functionalized copper nanoparticles was synthesized by a hydrothermal method and used for cysteine (Cys) detection in serum. The MBI-CuNPs probe exhibits strong fluorescence emission at 415 nm under the excitation at 200 nm, which is attributed to the metal-ligand charge transfer (MLCT) transition through the coordination of an MBI ligand and monovalent copper. Furthermore, the MBI-CuNPs probe has a high quenching fluorescence response to Cys, and shows a good linearity relationship with Cys in 0.05–65 µM, with a detection limit of 52 nM. Moreover, the MBI-CuNPs probe could eliminate the interference of biological mercaptan Hcy and GSH with a similar structure and reaction properties, due to the strong electron-donating ability of Cys, which can quench the fluorescence of the MBI-CuNPs probe. The MBI-CuNPs probe was applied to the analysis of Cys in real serum, and the absolute recovery rate was as high as 90.23–97.00%. Such a fluorescent probe with high sensitivity and selectivity has potential applications for the early prevention of various diseases caused by abnormal Cys levels. MDPI 2023-06-22 /pmc/articles/PMC10346489/ /pubmed/37447664 http://dx.doi.org/10.3390/s23135814 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Jing
Dou, Xiaozong
Zhang, Hongyan
2-Mercaptobenzimidazole Functionalized Copper Nanoparticles Fluorescence Probe for Sensitivity and Selectivity Detection of Cys in Serum
title 2-Mercaptobenzimidazole Functionalized Copper Nanoparticles Fluorescence Probe for Sensitivity and Selectivity Detection of Cys in Serum
title_full 2-Mercaptobenzimidazole Functionalized Copper Nanoparticles Fluorescence Probe for Sensitivity and Selectivity Detection of Cys in Serum
title_fullStr 2-Mercaptobenzimidazole Functionalized Copper Nanoparticles Fluorescence Probe for Sensitivity and Selectivity Detection of Cys in Serum
title_full_unstemmed 2-Mercaptobenzimidazole Functionalized Copper Nanoparticles Fluorescence Probe for Sensitivity and Selectivity Detection of Cys in Serum
title_short 2-Mercaptobenzimidazole Functionalized Copper Nanoparticles Fluorescence Probe for Sensitivity and Selectivity Detection of Cys in Serum
title_sort 2-mercaptobenzimidazole functionalized copper nanoparticles fluorescence probe for sensitivity and selectivity detection of cys in serum
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10346489/
https://www.ncbi.nlm.nih.gov/pubmed/37447664
http://dx.doi.org/10.3390/s23135814
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