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Identification of the Regulatory Role of SlWRKYs in Tomato Defense against Meloidogyne incognita
Root-knot nematode (RKN) infections are among the most serious soil-borne diseases in the world, and tomato is a common host of RKNs. WRKY transcription factors are involved in complex, diverse biological processes in plants. In a previous study, a resistant variety, LA3858 (Mi-3/Mi-3), was treated...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10346644/ https://www.ncbi.nlm.nih.gov/pubmed/37446977 http://dx.doi.org/10.3390/plants12132416 |
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author | Nie, Weidan Liu, Lili Chen, Yinxia Luo, Mingyin Feng, Chenghao Wang, Chaonan Yang, Zhongmin Du, Chong |
author_facet | Nie, Weidan Liu, Lili Chen, Yinxia Luo, Mingyin Feng, Chenghao Wang, Chaonan Yang, Zhongmin Du, Chong |
author_sort | Nie, Weidan |
collection | PubMed |
description | Root-knot nematode (RKN) infections are among the most serious soil-borne diseases in the world, and tomato is a common host of RKNs. WRKY transcription factors are involved in complex, diverse biological processes in plants. In a previous study, a resistant variety, LA3858 (Mi-3/Mi-3), was treated at different soil temperatures before RNA-seq, and six differentially expressed genes (DEGs) encoding WRKY proteins were screened. In this study, cloning and sequencing were used to identify six target DEGs encoding SlWRKY1, SlWRKY13, SlWRKY30, SlWRKY41, SlWRKY46, and SlWRKY80. Conserved domain identification and phylogenetic tree analysis showed that SlWRKY1, SlWRKY13, and SlWRKY46 have similar functions and are mainly involved in plant growth and development and abiotic stress responses. SlWRKY30 and SlWRKY41 share high homology, while AtWRKY46 and AtWRKY70, which are highly homologous to SlWRKY80, play an important role in the disease resistance of A. thaliana. Considering these findings combined with the high level of SlWRKY80 expression observed in the roots and leaves of the resistant variety Motelle (Mi-1/Mi-1) and the continuous upregulation of SlWRKY80 expression in the roots after inoculation of Motelle with M. incognita, it is speculated that SlWRKY80 plays an important role in the Mi-1-mediated disease resistance pathway. Further study revealed that SlWRKY80 is a typical nuclear-localized protein, and a virus-induced gene silencing (VIGS) assay verified that SlWRKY80 is involved in tomato resistance to RKNs as a positive regulator. SA and JA signals play an important role in Mi-1-mediated resistance to RKNs. SlWRKY80 was able to respond rapidly to treatment with both plant hormones, which indicated that SlWRKY80 might be involved in disease resistance regulation through various immune pathways. |
format | Online Article Text |
id | pubmed-10346644 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-103466442023-07-15 Identification of the Regulatory Role of SlWRKYs in Tomato Defense against Meloidogyne incognita Nie, Weidan Liu, Lili Chen, Yinxia Luo, Mingyin Feng, Chenghao Wang, Chaonan Yang, Zhongmin Du, Chong Plants (Basel) Article Root-knot nematode (RKN) infections are among the most serious soil-borne diseases in the world, and tomato is a common host of RKNs. WRKY transcription factors are involved in complex, diverse biological processes in plants. In a previous study, a resistant variety, LA3858 (Mi-3/Mi-3), was treated at different soil temperatures before RNA-seq, and six differentially expressed genes (DEGs) encoding WRKY proteins were screened. In this study, cloning and sequencing were used to identify six target DEGs encoding SlWRKY1, SlWRKY13, SlWRKY30, SlWRKY41, SlWRKY46, and SlWRKY80. Conserved domain identification and phylogenetic tree analysis showed that SlWRKY1, SlWRKY13, and SlWRKY46 have similar functions and are mainly involved in plant growth and development and abiotic stress responses. SlWRKY30 and SlWRKY41 share high homology, while AtWRKY46 and AtWRKY70, which are highly homologous to SlWRKY80, play an important role in the disease resistance of A. thaliana. Considering these findings combined with the high level of SlWRKY80 expression observed in the roots and leaves of the resistant variety Motelle (Mi-1/Mi-1) and the continuous upregulation of SlWRKY80 expression in the roots after inoculation of Motelle with M. incognita, it is speculated that SlWRKY80 plays an important role in the Mi-1-mediated disease resistance pathway. Further study revealed that SlWRKY80 is a typical nuclear-localized protein, and a virus-induced gene silencing (VIGS) assay verified that SlWRKY80 is involved in tomato resistance to RKNs as a positive regulator. SA and JA signals play an important role in Mi-1-mediated resistance to RKNs. SlWRKY80 was able to respond rapidly to treatment with both plant hormones, which indicated that SlWRKY80 might be involved in disease resistance regulation through various immune pathways. MDPI 2023-06-22 /pmc/articles/PMC10346644/ /pubmed/37446977 http://dx.doi.org/10.3390/plants12132416 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Nie, Weidan Liu, Lili Chen, Yinxia Luo, Mingyin Feng, Chenghao Wang, Chaonan Yang, Zhongmin Du, Chong Identification of the Regulatory Role of SlWRKYs in Tomato Defense against Meloidogyne incognita |
title | Identification of the Regulatory Role of SlWRKYs in Tomato Defense against Meloidogyne incognita
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title_full | Identification of the Regulatory Role of SlWRKYs in Tomato Defense against Meloidogyne incognita
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title_fullStr | Identification of the Regulatory Role of SlWRKYs in Tomato Defense against Meloidogyne incognita
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title_full_unstemmed | Identification of the Regulatory Role of SlWRKYs in Tomato Defense against Meloidogyne incognita
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title_short | Identification of the Regulatory Role of SlWRKYs in Tomato Defense against Meloidogyne incognita
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title_sort | identification of the regulatory role of slwrkys in tomato defense against meloidogyne incognita |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10346644/ https://www.ncbi.nlm.nih.gov/pubmed/37446977 http://dx.doi.org/10.3390/plants12132416 |
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