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Identification of the Regulatory Role of SlWRKYs in Tomato Defense against Meloidogyne incognita

Root-knot nematode (RKN) infections are among the most serious soil-borne diseases in the world, and tomato is a common host of RKNs. WRKY transcription factors are involved in complex, diverse biological processes in plants. In a previous study, a resistant variety, LA3858 (Mi-3/Mi-3), was treated...

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Autores principales: Nie, Weidan, Liu, Lili, Chen, Yinxia, Luo, Mingyin, Feng, Chenghao, Wang, Chaonan, Yang, Zhongmin, Du, Chong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10346644/
https://www.ncbi.nlm.nih.gov/pubmed/37446977
http://dx.doi.org/10.3390/plants12132416
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author Nie, Weidan
Liu, Lili
Chen, Yinxia
Luo, Mingyin
Feng, Chenghao
Wang, Chaonan
Yang, Zhongmin
Du, Chong
author_facet Nie, Weidan
Liu, Lili
Chen, Yinxia
Luo, Mingyin
Feng, Chenghao
Wang, Chaonan
Yang, Zhongmin
Du, Chong
author_sort Nie, Weidan
collection PubMed
description Root-knot nematode (RKN) infections are among the most serious soil-borne diseases in the world, and tomato is a common host of RKNs. WRKY transcription factors are involved in complex, diverse biological processes in plants. In a previous study, a resistant variety, LA3858 (Mi-3/Mi-3), was treated at different soil temperatures before RNA-seq, and six differentially expressed genes (DEGs) encoding WRKY proteins were screened. In this study, cloning and sequencing were used to identify six target DEGs encoding SlWRKY1, SlWRKY13, SlWRKY30, SlWRKY41, SlWRKY46, and SlWRKY80. Conserved domain identification and phylogenetic tree analysis showed that SlWRKY1, SlWRKY13, and SlWRKY46 have similar functions and are mainly involved in plant growth and development and abiotic stress responses. SlWRKY30 and SlWRKY41 share high homology, while AtWRKY46 and AtWRKY70, which are highly homologous to SlWRKY80, play an important role in the disease resistance of A. thaliana. Considering these findings combined with the high level of SlWRKY80 expression observed in the roots and leaves of the resistant variety Motelle (Mi-1/Mi-1) and the continuous upregulation of SlWRKY80 expression in the roots after inoculation of Motelle with M. incognita, it is speculated that SlWRKY80 plays an important role in the Mi-1-mediated disease resistance pathway. Further study revealed that SlWRKY80 is a typical nuclear-localized protein, and a virus-induced gene silencing (VIGS) assay verified that SlWRKY80 is involved in tomato resistance to RKNs as a positive regulator. SA and JA signals play an important role in Mi-1-mediated resistance to RKNs. SlWRKY80 was able to respond rapidly to treatment with both plant hormones, which indicated that SlWRKY80 might be involved in disease resistance regulation through various immune pathways.
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spelling pubmed-103466442023-07-15 Identification of the Regulatory Role of SlWRKYs in Tomato Defense against Meloidogyne incognita Nie, Weidan Liu, Lili Chen, Yinxia Luo, Mingyin Feng, Chenghao Wang, Chaonan Yang, Zhongmin Du, Chong Plants (Basel) Article Root-knot nematode (RKN) infections are among the most serious soil-borne diseases in the world, and tomato is a common host of RKNs. WRKY transcription factors are involved in complex, diverse biological processes in plants. In a previous study, a resistant variety, LA3858 (Mi-3/Mi-3), was treated at different soil temperatures before RNA-seq, and six differentially expressed genes (DEGs) encoding WRKY proteins were screened. In this study, cloning and sequencing were used to identify six target DEGs encoding SlWRKY1, SlWRKY13, SlWRKY30, SlWRKY41, SlWRKY46, and SlWRKY80. Conserved domain identification and phylogenetic tree analysis showed that SlWRKY1, SlWRKY13, and SlWRKY46 have similar functions and are mainly involved in plant growth and development and abiotic stress responses. SlWRKY30 and SlWRKY41 share high homology, while AtWRKY46 and AtWRKY70, which are highly homologous to SlWRKY80, play an important role in the disease resistance of A. thaliana. Considering these findings combined with the high level of SlWRKY80 expression observed in the roots and leaves of the resistant variety Motelle (Mi-1/Mi-1) and the continuous upregulation of SlWRKY80 expression in the roots after inoculation of Motelle with M. incognita, it is speculated that SlWRKY80 plays an important role in the Mi-1-mediated disease resistance pathway. Further study revealed that SlWRKY80 is a typical nuclear-localized protein, and a virus-induced gene silencing (VIGS) assay verified that SlWRKY80 is involved in tomato resistance to RKNs as a positive regulator. SA and JA signals play an important role in Mi-1-mediated resistance to RKNs. SlWRKY80 was able to respond rapidly to treatment with both plant hormones, which indicated that SlWRKY80 might be involved in disease resistance regulation through various immune pathways. MDPI 2023-06-22 /pmc/articles/PMC10346644/ /pubmed/37446977 http://dx.doi.org/10.3390/plants12132416 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Nie, Weidan
Liu, Lili
Chen, Yinxia
Luo, Mingyin
Feng, Chenghao
Wang, Chaonan
Yang, Zhongmin
Du, Chong
Identification of the Regulatory Role of SlWRKYs in Tomato Defense against Meloidogyne incognita
title Identification of the Regulatory Role of SlWRKYs in Tomato Defense against Meloidogyne incognita
title_full Identification of the Regulatory Role of SlWRKYs in Tomato Defense against Meloidogyne incognita
title_fullStr Identification of the Regulatory Role of SlWRKYs in Tomato Defense against Meloidogyne incognita
title_full_unstemmed Identification of the Regulatory Role of SlWRKYs in Tomato Defense against Meloidogyne incognita
title_short Identification of the Regulatory Role of SlWRKYs in Tomato Defense against Meloidogyne incognita
title_sort identification of the regulatory role of slwrkys in tomato defense against meloidogyne incognita
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10346644/
https://www.ncbi.nlm.nih.gov/pubmed/37446977
http://dx.doi.org/10.3390/plants12132416
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