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Anti-Adipogenic Activity of Rhaponticum carthamoides and Its Secondary Metabolites

Besides their common use as an adaptogen, Rhaponticum carthamoides (Willd.) Iljin. rhizome and its root extract (RCE) are also reported to beneficially affect lipid metabolism. The main characteristic secondary metabolites of RCE are phytoecdysteroids. In order to determine an RCE’s phytoecdysteroid...

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Autores principales: Todorova, Velislava, Savova, Martina S., Ivanova, Stanislava, Ivanov, Kalin, Georgiev, Milen I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10346812/
https://www.ncbi.nlm.nih.gov/pubmed/37447387
http://dx.doi.org/10.3390/nu15133061
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author Todorova, Velislava
Savova, Martina S.
Ivanova, Stanislava
Ivanov, Kalin
Georgiev, Milen I.
author_facet Todorova, Velislava
Savova, Martina S.
Ivanova, Stanislava
Ivanov, Kalin
Georgiev, Milen I.
author_sort Todorova, Velislava
collection PubMed
description Besides their common use as an adaptogen, Rhaponticum carthamoides (Willd.) Iljin. rhizome and its root extract (RCE) are also reported to beneficially affect lipid metabolism. The main characteristic secondary metabolites of RCE are phytoecdysteroids. In order to determine an RCE’s phytoecdysteroid profile, a novel, sensitive, and robust high-performance thin-layer chromatography (HPTLC) method was developed and validated. Moreover, a comparative analysis was conducted to investigate the effects of RCE and its secondary metabolites on adipogenesis and adipolysis. The evaluation of the anti-adipogenic and lipolytic effects was performed using human Simpson–Golabi–Behmel syndrome cells, where lipid staining and measurement of released glycerol and free fatty acids were employed. The HPTLC method confirmed the presence of 20-hydroxyecdysone (20E), ponasterone A (PA), and turkesterone (TU) in RCE. The observed results revealed that RCE, 20E, and TU significantly reduced lipid accumulation in human adipocytes, demonstrating their anti-adipogenic activity. Moreover, RCE and 20E were found to effectively stimulate basal lipolysis. However, no significant effects were observed with PA and TU applications. Based on our findings, RCE and 20E affect both lipogenesis and lipolysis, while TU only restrains adipogenesis. These results are fundamental for further investigations.
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spelling pubmed-103468122023-07-15 Anti-Adipogenic Activity of Rhaponticum carthamoides and Its Secondary Metabolites Todorova, Velislava Savova, Martina S. Ivanova, Stanislava Ivanov, Kalin Georgiev, Milen I. Nutrients Article Besides their common use as an adaptogen, Rhaponticum carthamoides (Willd.) Iljin. rhizome and its root extract (RCE) are also reported to beneficially affect lipid metabolism. The main characteristic secondary metabolites of RCE are phytoecdysteroids. In order to determine an RCE’s phytoecdysteroid profile, a novel, sensitive, and robust high-performance thin-layer chromatography (HPTLC) method was developed and validated. Moreover, a comparative analysis was conducted to investigate the effects of RCE and its secondary metabolites on adipogenesis and adipolysis. The evaluation of the anti-adipogenic and lipolytic effects was performed using human Simpson–Golabi–Behmel syndrome cells, where lipid staining and measurement of released glycerol and free fatty acids were employed. The HPTLC method confirmed the presence of 20-hydroxyecdysone (20E), ponasterone A (PA), and turkesterone (TU) in RCE. The observed results revealed that RCE, 20E, and TU significantly reduced lipid accumulation in human adipocytes, demonstrating their anti-adipogenic activity. Moreover, RCE and 20E were found to effectively stimulate basal lipolysis. However, no significant effects were observed with PA and TU applications. Based on our findings, RCE and 20E affect both lipogenesis and lipolysis, while TU only restrains adipogenesis. These results are fundamental for further investigations. MDPI 2023-07-07 /pmc/articles/PMC10346812/ /pubmed/37447387 http://dx.doi.org/10.3390/nu15133061 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Todorova, Velislava
Savova, Martina S.
Ivanova, Stanislava
Ivanov, Kalin
Georgiev, Milen I.
Anti-Adipogenic Activity of Rhaponticum carthamoides and Its Secondary Metabolites
title Anti-Adipogenic Activity of Rhaponticum carthamoides and Its Secondary Metabolites
title_full Anti-Adipogenic Activity of Rhaponticum carthamoides and Its Secondary Metabolites
title_fullStr Anti-Adipogenic Activity of Rhaponticum carthamoides and Its Secondary Metabolites
title_full_unstemmed Anti-Adipogenic Activity of Rhaponticum carthamoides and Its Secondary Metabolites
title_short Anti-Adipogenic Activity of Rhaponticum carthamoides and Its Secondary Metabolites
title_sort anti-adipogenic activity of rhaponticum carthamoides and its secondary metabolites
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10346812/
https://www.ncbi.nlm.nih.gov/pubmed/37447387
http://dx.doi.org/10.3390/nu15133061
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