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E2F3 accelerates the stemness of colon cancer cells by activating the STAT3 pathway
INTRODUCTION: Colon cancer is one of the most prevalent malignancies and causes of cancer-related deaths worldwide. Thus, further research is required to explicate the latent molecular mechanisms and look for novel biomarkers. E2F3 has been confirmed to be an oncogene in a variety of cancers. Howeve...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10346838/ https://www.ncbi.nlm.nih.gov/pubmed/37456248 http://dx.doi.org/10.3389/fonc.2023.1203712 |
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author | Gao, Qingkun An, Ke Lv, Zhe Wang, Yanzhao Ding, Changmin Huang, Wensheng |
author_facet | Gao, Qingkun An, Ke Lv, Zhe Wang, Yanzhao Ding, Changmin Huang, Wensheng |
author_sort | Gao, Qingkun |
collection | PubMed |
description | INTRODUCTION: Colon cancer is one of the most prevalent malignancies and causes of cancer-related deaths worldwide. Thus, further research is required to explicate the latent molecular mechanisms and look for novel biomarkers. E2F3 has been confirmed to be an oncogene in a variety of cancers. However, the particular regulation of E2F3 in colon cancer needs further investigation. METHODS: The self-renewal ability was detected through a sphere formation assay. The tumorigenic ability was measured through nude mice in vivo assay. The protein expression of genes was examined through a Western blot. The expression of E2F3 in tumor tissues was detected through an IHC assay. The resistance to cisplatin was assessed through the CCK-8 assay. The cell migration and invasion abilities were measured after upregulating or suppressing E2F3 through the Transwell assay. RESULTS: Results uncovered that E2F3 was upregulated in spheroid cells. In addition, E2F3 facilitates stemness in colon cancer. Moreover, E2F3 facilitated colon cancer cell migration and invasion. Finally, it was revealed that E2F3 affected the STAT3 pathway to modulate stemness in colon cancer. E2F3 served as a promoter regulator in colon cancer, aggravating tumorigenesis and stemness in colon cancer progression through the STAT3 pathway. CONCLUSION: E2F3 may be a useful biomarker for anticancer treatment in colon cancer. |
format | Online Article Text |
id | pubmed-10346838 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-103468382023-07-15 E2F3 accelerates the stemness of colon cancer cells by activating the STAT3 pathway Gao, Qingkun An, Ke Lv, Zhe Wang, Yanzhao Ding, Changmin Huang, Wensheng Front Oncol Oncology INTRODUCTION: Colon cancer is one of the most prevalent malignancies and causes of cancer-related deaths worldwide. Thus, further research is required to explicate the latent molecular mechanisms and look for novel biomarkers. E2F3 has been confirmed to be an oncogene in a variety of cancers. However, the particular regulation of E2F3 in colon cancer needs further investigation. METHODS: The self-renewal ability was detected through a sphere formation assay. The tumorigenic ability was measured through nude mice in vivo assay. The protein expression of genes was examined through a Western blot. The expression of E2F3 in tumor tissues was detected through an IHC assay. The resistance to cisplatin was assessed through the CCK-8 assay. The cell migration and invasion abilities were measured after upregulating or suppressing E2F3 through the Transwell assay. RESULTS: Results uncovered that E2F3 was upregulated in spheroid cells. In addition, E2F3 facilitates stemness in colon cancer. Moreover, E2F3 facilitated colon cancer cell migration and invasion. Finally, it was revealed that E2F3 affected the STAT3 pathway to modulate stemness in colon cancer. E2F3 served as a promoter regulator in colon cancer, aggravating tumorigenesis and stemness in colon cancer progression through the STAT3 pathway. CONCLUSION: E2F3 may be a useful biomarker for anticancer treatment in colon cancer. Frontiers Media S.A. 2023-06-29 /pmc/articles/PMC10346838/ /pubmed/37456248 http://dx.doi.org/10.3389/fonc.2023.1203712 Text en Copyright © 2023 Gao, An, Lv, Wang, Ding and Huang https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Oncology Gao, Qingkun An, Ke Lv, Zhe Wang, Yanzhao Ding, Changmin Huang, Wensheng E2F3 accelerates the stemness of colon cancer cells by activating the STAT3 pathway |
title | E2F3 accelerates the stemness of colon cancer cells by activating the STAT3 pathway |
title_full | E2F3 accelerates the stemness of colon cancer cells by activating the STAT3 pathway |
title_fullStr | E2F3 accelerates the stemness of colon cancer cells by activating the STAT3 pathway |
title_full_unstemmed | E2F3 accelerates the stemness of colon cancer cells by activating the STAT3 pathway |
title_short | E2F3 accelerates the stemness of colon cancer cells by activating the STAT3 pathway |
title_sort | e2f3 accelerates the stemness of colon cancer cells by activating the stat3 pathway |
topic | Oncology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10346838/ https://www.ncbi.nlm.nih.gov/pubmed/37456248 http://dx.doi.org/10.3389/fonc.2023.1203712 |
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