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Coelenterazine Indicators for the Specific Imaging of Human and Bovine Serum Albumins

Albumin assays in serum are important for the prognostic assessment of many life-threatening diseases, such as heart failure, liver disease, malnutrition, inflammatory bowel disease, infections, and kidney disease. In this study, synthetic coelenterazine (CTZ) indicators are developed to quantitativ...

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Autores principales: Kim, Sung-Bae, Kamiya, Genta, Furuta, Tadaomi, Kitada, Nobuo, Maki, Shojiro A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10346935/
https://www.ncbi.nlm.nih.gov/pubmed/37447868
http://dx.doi.org/10.3390/s23136020
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author Kim, Sung-Bae
Kamiya, Genta
Furuta, Tadaomi
Kitada, Nobuo
Maki, Shojiro A.
author_facet Kim, Sung-Bae
Kamiya, Genta
Furuta, Tadaomi
Kitada, Nobuo
Maki, Shojiro A.
author_sort Kim, Sung-Bae
collection PubMed
description Albumin assays in serum are important for the prognostic assessment of many life-threatening diseases, such as heart failure, liver disease, malnutrition, inflammatory bowel disease, infections, and kidney disease. In this study, synthetic coelenterazine (CTZ) indicators are developed to quantitatively illuminate human and bovine serum albumins (HSA and BSA) with high specificity. Their functional groups were chemically modified to specifically emit luminescence with HSA and BSA. The CTZ indicators were characterized by assaying the most abundant serum proteins and found that the CTZ indicators S6 and S6h were highly specific to HSA and BSA, respectively. Their colors were dramatically converted from blue, peaked at 480 nm, to yellowish green, peaked at 535 nm, according to the HSA–BSA mixing ratios, wherein the origins and mixing levels of the albumins can be easily determined by their colors and peak positions. The kinetic properties of HSA and BSA were investigated in detail, confirming that the serum albumins catalyze the CTZ indicators, which act as pseudo-luciferases. The catalytic reactions were efficiently inhibited by specific inhibitors, blocking the drug-binding sites I and II of HSA and BSA. Finally, the utility of the CTZ indicators was demonstrated through a quantitative imaging of the real fetal bovine serum (FBS). This study is the first example to show that the CTZ indicators specify HSA and BSA with different colors. This study contributes to the expansion of the toolbox of optical indicators, which efficiently assays serum proteins in physiological samples. Considering that these CTZ indicators immediately report quantitative optical signals with high specificity, they provide solutions to conventional technical hurdles on point-of-care assays of serum albumins.
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spelling pubmed-103469352023-07-15 Coelenterazine Indicators for the Specific Imaging of Human and Bovine Serum Albumins Kim, Sung-Bae Kamiya, Genta Furuta, Tadaomi Kitada, Nobuo Maki, Shojiro A. Sensors (Basel) Article Albumin assays in serum are important for the prognostic assessment of many life-threatening diseases, such as heart failure, liver disease, malnutrition, inflammatory bowel disease, infections, and kidney disease. In this study, synthetic coelenterazine (CTZ) indicators are developed to quantitatively illuminate human and bovine serum albumins (HSA and BSA) with high specificity. Their functional groups were chemically modified to specifically emit luminescence with HSA and BSA. The CTZ indicators were characterized by assaying the most abundant serum proteins and found that the CTZ indicators S6 and S6h were highly specific to HSA and BSA, respectively. Their colors were dramatically converted from blue, peaked at 480 nm, to yellowish green, peaked at 535 nm, according to the HSA–BSA mixing ratios, wherein the origins and mixing levels of the albumins can be easily determined by their colors and peak positions. The kinetic properties of HSA and BSA were investigated in detail, confirming that the serum albumins catalyze the CTZ indicators, which act as pseudo-luciferases. The catalytic reactions were efficiently inhibited by specific inhibitors, blocking the drug-binding sites I and II of HSA and BSA. Finally, the utility of the CTZ indicators was demonstrated through a quantitative imaging of the real fetal bovine serum (FBS). This study is the first example to show that the CTZ indicators specify HSA and BSA with different colors. This study contributes to the expansion of the toolbox of optical indicators, which efficiently assays serum proteins in physiological samples. Considering that these CTZ indicators immediately report quantitative optical signals with high specificity, they provide solutions to conventional technical hurdles on point-of-care assays of serum albumins. MDPI 2023-06-29 /pmc/articles/PMC10346935/ /pubmed/37447868 http://dx.doi.org/10.3390/s23136020 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kim, Sung-Bae
Kamiya, Genta
Furuta, Tadaomi
Kitada, Nobuo
Maki, Shojiro A.
Coelenterazine Indicators for the Specific Imaging of Human and Bovine Serum Albumins
title Coelenterazine Indicators for the Specific Imaging of Human and Bovine Serum Albumins
title_full Coelenterazine Indicators for the Specific Imaging of Human and Bovine Serum Albumins
title_fullStr Coelenterazine Indicators for the Specific Imaging of Human and Bovine Serum Albumins
title_full_unstemmed Coelenterazine Indicators for the Specific Imaging of Human and Bovine Serum Albumins
title_short Coelenterazine Indicators for the Specific Imaging of Human and Bovine Serum Albumins
title_sort coelenterazine indicators for the specific imaging of human and bovine serum albumins
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10346935/
https://www.ncbi.nlm.nih.gov/pubmed/37447868
http://dx.doi.org/10.3390/s23136020
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