Cargando…
Wound Healing Effect of Supercritical Carbon Dioxide Datura metel L. Leaves Extracts: An In Vitro Study of Anti-Inflammation, Cell Migration, MMP-2 Inhibition, and the Modulation of the Sonic Hedgehog Pathway in Human Fibroblasts
Datura metel L. (thorn apple) has been used in Thai folk wisdom for wound care. In this study, we chose supercritical carbon dioxide extraction (scCO(2)) to develop crude extraction from the leaves of the thorn apple. The phytochemical profiles were observed using liquid chromatography–quadrupole ti...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10346957/ https://www.ncbi.nlm.nih.gov/pubmed/37447107 http://dx.doi.org/10.3390/plants12132546 |
_version_ | 1785073435892776960 |
---|---|
author | Ruksiriwanich, Warintorn Linsaenkart, Pichchapa Muangsanguan, Anurak Sringarm, Korawan Jantrawut, Pensak Arjin, Chaiwat Sommano, Sarana Rose Phimolsiripol, Yuthana Barba, Francisco J. |
author_facet | Ruksiriwanich, Warintorn Linsaenkart, Pichchapa Muangsanguan, Anurak Sringarm, Korawan Jantrawut, Pensak Arjin, Chaiwat Sommano, Sarana Rose Phimolsiripol, Yuthana Barba, Francisco J. |
author_sort | Ruksiriwanich, Warintorn |
collection | PubMed |
description | Datura metel L. (thorn apple) has been used in Thai folk wisdom for wound care. In this study, we chose supercritical carbon dioxide extraction (scCO(2)) to develop crude extraction from the leaves of the thorn apple. The phytochemical profiles were observed using liquid chromatography–quadrupole time-of-flight mass spectrometry (LC-QTOF-MS). The biological activities of D. metel were performed through antioxidant assays, anti-inflammation based on the Griess reaction, the migration assay, the expression of matrix metalloproteinase-2 (MMP-2), and regulatory genes in fibroblasts. Dm1 and Dm2 extracts were obtained from scCO(2) procedures at different pressures of 300 and 500 bar, respectively. Bioactive compounds, including farnesyl acetone, schisanhenol B, and loliolide, were identified in both extracts. The antioxidant properties of both D. metel extracts were comparable to those of l-ascorbic acid in hydrogen peroxide-induced fibroblasts with no significant difference. Additionally, Dm1 and Dm2 significantly inhibited the nitrite production levels of 1.23 ± 0.19 and 1.52 ± 0.05 μM, respectively, against the lipopolysaccharide-treated group (3.82 ± 0.39 μM). Interestingly, Dm1 obviously demonstrated the percentage of wound closure with 58.46 ± 7.61 and 82.62 ± 6.66% after 36 and 48 h of treatment, which were comparable to the commercial deproteinized dialysate from the calf blood extract. Moreover, both extracts were comparable to l-ascorbic acid treatment in their ability to suppress the expression of MMP-2: an enzyme that breaks down collagen. The gene expressions of SHH, SMO, and GLI1 that control the sonic hedgehog pathway were also clearly upregulated by Dm1. Consequently, the scCO(2) technique could be applied in D. metel extraction and contribute to potentially effective wound closure. |
format | Online Article Text |
id | pubmed-10346957 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-103469572023-07-15 Wound Healing Effect of Supercritical Carbon Dioxide Datura metel L. Leaves Extracts: An In Vitro Study of Anti-Inflammation, Cell Migration, MMP-2 Inhibition, and the Modulation of the Sonic Hedgehog Pathway in Human Fibroblasts Ruksiriwanich, Warintorn Linsaenkart, Pichchapa Muangsanguan, Anurak Sringarm, Korawan Jantrawut, Pensak Arjin, Chaiwat Sommano, Sarana Rose Phimolsiripol, Yuthana Barba, Francisco J. Plants (Basel) Article Datura metel L. (thorn apple) has been used in Thai folk wisdom for wound care. In this study, we chose supercritical carbon dioxide extraction (scCO(2)) to develop crude extraction from the leaves of the thorn apple. The phytochemical profiles were observed using liquid chromatography–quadrupole time-of-flight mass spectrometry (LC-QTOF-MS). The biological activities of D. metel were performed through antioxidant assays, anti-inflammation based on the Griess reaction, the migration assay, the expression of matrix metalloproteinase-2 (MMP-2), and regulatory genes in fibroblasts. Dm1 and Dm2 extracts were obtained from scCO(2) procedures at different pressures of 300 and 500 bar, respectively. Bioactive compounds, including farnesyl acetone, schisanhenol B, and loliolide, were identified in both extracts. The antioxidant properties of both D. metel extracts were comparable to those of l-ascorbic acid in hydrogen peroxide-induced fibroblasts with no significant difference. Additionally, Dm1 and Dm2 significantly inhibited the nitrite production levels of 1.23 ± 0.19 and 1.52 ± 0.05 μM, respectively, against the lipopolysaccharide-treated group (3.82 ± 0.39 μM). Interestingly, Dm1 obviously demonstrated the percentage of wound closure with 58.46 ± 7.61 and 82.62 ± 6.66% after 36 and 48 h of treatment, which were comparable to the commercial deproteinized dialysate from the calf blood extract. Moreover, both extracts were comparable to l-ascorbic acid treatment in their ability to suppress the expression of MMP-2: an enzyme that breaks down collagen. The gene expressions of SHH, SMO, and GLI1 that control the sonic hedgehog pathway were also clearly upregulated by Dm1. Consequently, the scCO(2) technique could be applied in D. metel extraction and contribute to potentially effective wound closure. MDPI 2023-07-04 /pmc/articles/PMC10346957/ /pubmed/37447107 http://dx.doi.org/10.3390/plants12132546 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ruksiriwanich, Warintorn Linsaenkart, Pichchapa Muangsanguan, Anurak Sringarm, Korawan Jantrawut, Pensak Arjin, Chaiwat Sommano, Sarana Rose Phimolsiripol, Yuthana Barba, Francisco J. Wound Healing Effect of Supercritical Carbon Dioxide Datura metel L. Leaves Extracts: An In Vitro Study of Anti-Inflammation, Cell Migration, MMP-2 Inhibition, and the Modulation of the Sonic Hedgehog Pathway in Human Fibroblasts |
title | Wound Healing Effect of Supercritical Carbon Dioxide Datura metel L. Leaves Extracts: An In Vitro Study of Anti-Inflammation, Cell Migration, MMP-2 Inhibition, and the Modulation of the Sonic Hedgehog Pathway in Human Fibroblasts |
title_full | Wound Healing Effect of Supercritical Carbon Dioxide Datura metel L. Leaves Extracts: An In Vitro Study of Anti-Inflammation, Cell Migration, MMP-2 Inhibition, and the Modulation of the Sonic Hedgehog Pathway in Human Fibroblasts |
title_fullStr | Wound Healing Effect of Supercritical Carbon Dioxide Datura metel L. Leaves Extracts: An In Vitro Study of Anti-Inflammation, Cell Migration, MMP-2 Inhibition, and the Modulation of the Sonic Hedgehog Pathway in Human Fibroblasts |
title_full_unstemmed | Wound Healing Effect of Supercritical Carbon Dioxide Datura metel L. Leaves Extracts: An In Vitro Study of Anti-Inflammation, Cell Migration, MMP-2 Inhibition, and the Modulation of the Sonic Hedgehog Pathway in Human Fibroblasts |
title_short | Wound Healing Effect of Supercritical Carbon Dioxide Datura metel L. Leaves Extracts: An In Vitro Study of Anti-Inflammation, Cell Migration, MMP-2 Inhibition, and the Modulation of the Sonic Hedgehog Pathway in Human Fibroblasts |
title_sort | wound healing effect of supercritical carbon dioxide datura metel l. leaves extracts: an in vitro study of anti-inflammation, cell migration, mmp-2 inhibition, and the modulation of the sonic hedgehog pathway in human fibroblasts |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10346957/ https://www.ncbi.nlm.nih.gov/pubmed/37447107 http://dx.doi.org/10.3390/plants12132546 |
work_keys_str_mv | AT ruksiriwanichwarintorn woundhealingeffectofsupercriticalcarbondioxidedaturametellleavesextractsaninvitrostudyofantiinflammationcellmigrationmmp2inhibitionandthemodulationofthesonichedgehogpathwayinhumanfibroblasts AT linsaenkartpichchapa woundhealingeffectofsupercriticalcarbondioxidedaturametellleavesextractsaninvitrostudyofantiinflammationcellmigrationmmp2inhibitionandthemodulationofthesonichedgehogpathwayinhumanfibroblasts AT muangsanguananurak woundhealingeffectofsupercriticalcarbondioxidedaturametellleavesextractsaninvitrostudyofantiinflammationcellmigrationmmp2inhibitionandthemodulationofthesonichedgehogpathwayinhumanfibroblasts AT sringarmkorawan woundhealingeffectofsupercriticalcarbondioxidedaturametellleavesextractsaninvitrostudyofantiinflammationcellmigrationmmp2inhibitionandthemodulationofthesonichedgehogpathwayinhumanfibroblasts AT jantrawutpensak woundhealingeffectofsupercriticalcarbondioxidedaturametellleavesextractsaninvitrostudyofantiinflammationcellmigrationmmp2inhibitionandthemodulationofthesonichedgehogpathwayinhumanfibroblasts AT arjinchaiwat woundhealingeffectofsupercriticalcarbondioxidedaturametellleavesextractsaninvitrostudyofantiinflammationcellmigrationmmp2inhibitionandthemodulationofthesonichedgehogpathwayinhumanfibroblasts AT sommanosaranarose woundhealingeffectofsupercriticalcarbondioxidedaturametellleavesextractsaninvitrostudyofantiinflammationcellmigrationmmp2inhibitionandthemodulationofthesonichedgehogpathwayinhumanfibroblasts AT phimolsiripolyuthana woundhealingeffectofsupercriticalcarbondioxidedaturametellleavesextractsaninvitrostudyofantiinflammationcellmigrationmmp2inhibitionandthemodulationofthesonichedgehogpathwayinhumanfibroblasts AT barbafranciscoj woundhealingeffectofsupercriticalcarbondioxidedaturametellleavesextractsaninvitrostudyofantiinflammationcellmigrationmmp2inhibitionandthemodulationofthesonichedgehogpathwayinhumanfibroblasts |