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The Utilisation of Antarctic Microalgae Isolated from Paradise Bay (Antarctic Peninsula) in the Bioremediation of Diesel

Research has confirmed that the utilisation of Antarctic microorganisms, such as bacteria, yeasts and fungi, in the bioremediation of diesel may provide practical alternative approaches. However, to date there has been very little attention towards Antarctic microalgae as potential hydrocarbon degra...

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Autores principales: Zamree, Nur Diyanah, Puasa, Nurul Aini, Lim, Zheng Syuen, Wong, Chiew-Yen, Shaharuddin, Noor Azmi, Zakaria, Nur Nadhirah, Merican, Faradina, Convey, Peter, Ahmad, Syahida, Shaari, Hasrizal, Azmi, Alyza Azzura, Ahmad, Siti Aqlima, Zulkharnain, Azham
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10347017/
https://www.ncbi.nlm.nih.gov/pubmed/37447097
http://dx.doi.org/10.3390/plants12132536
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author Zamree, Nur Diyanah
Puasa, Nurul Aini
Lim, Zheng Syuen
Wong, Chiew-Yen
Shaharuddin, Noor Azmi
Zakaria, Nur Nadhirah
Merican, Faradina
Convey, Peter
Ahmad, Syahida
Shaari, Hasrizal
Azmi, Alyza Azzura
Ahmad, Siti Aqlima
Zulkharnain, Azham
author_facet Zamree, Nur Diyanah
Puasa, Nurul Aini
Lim, Zheng Syuen
Wong, Chiew-Yen
Shaharuddin, Noor Azmi
Zakaria, Nur Nadhirah
Merican, Faradina
Convey, Peter
Ahmad, Syahida
Shaari, Hasrizal
Azmi, Alyza Azzura
Ahmad, Siti Aqlima
Zulkharnain, Azham
author_sort Zamree, Nur Diyanah
collection PubMed
description Research has confirmed that the utilisation of Antarctic microorganisms, such as bacteria, yeasts and fungi, in the bioremediation of diesel may provide practical alternative approaches. However, to date there has been very little attention towards Antarctic microalgae as potential hydrocarbon degraders. Therefore, this study focused on the utilisation of an Antarctic microalga in the bioremediation of diesel. The studied microalgal strain was originally obtained from a freshwater ecosystem in Paradise Bay, western Antarctic Peninsula. When analysed in systems with and without aeration, this microalgal strain achieved a higher growth rate under aeration. To maintain the growth of this microalga optimally, a conventional one-factor-at a-time (OFAT) analysis was also conducted. Based on the optimized parameters, algal growth and diesel degradation performance was highest at pH 7.5 with 0.5 mg/L NaCl concentration and 0.5 g/L of NaNO(3) as a nitrogen source. This currently unidentified microalga flourished in the presence of diesel, with maximum algal cell numbers on day 7 of incubation in the presence of 1% v/v diesel. Chlorophyll a, b and carotenoid contents of the culture were greatest on day 9 of incubation. The diesel degradation achieved was 64.5% of the original concentration after 9 days. Gas chromatography analysis showed the complete mineralisation of C(7)–C(13) hydrocarbon chains. Fourier transform infrared spectroscopy analysis confirmed that strain WCY_AQ5_3 fully degraded the hydrocarbon with bioabsorption of the products. Morphological and molecular analyses suggested that this spherical, single-celled green microalga was a member of the genus Micractinium. The data obtained confirm that this microalga is a suitable candidate for further research into the degradation of diesel in Antarctica.
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spelling pubmed-103470172023-07-15 The Utilisation of Antarctic Microalgae Isolated from Paradise Bay (Antarctic Peninsula) in the Bioremediation of Diesel Zamree, Nur Diyanah Puasa, Nurul Aini Lim, Zheng Syuen Wong, Chiew-Yen Shaharuddin, Noor Azmi Zakaria, Nur Nadhirah Merican, Faradina Convey, Peter Ahmad, Syahida Shaari, Hasrizal Azmi, Alyza Azzura Ahmad, Siti Aqlima Zulkharnain, Azham Plants (Basel) Article Research has confirmed that the utilisation of Antarctic microorganisms, such as bacteria, yeasts and fungi, in the bioremediation of diesel may provide practical alternative approaches. However, to date there has been very little attention towards Antarctic microalgae as potential hydrocarbon degraders. Therefore, this study focused on the utilisation of an Antarctic microalga in the bioremediation of diesel. The studied microalgal strain was originally obtained from a freshwater ecosystem in Paradise Bay, western Antarctic Peninsula. When analysed in systems with and without aeration, this microalgal strain achieved a higher growth rate under aeration. To maintain the growth of this microalga optimally, a conventional one-factor-at a-time (OFAT) analysis was also conducted. Based on the optimized parameters, algal growth and diesel degradation performance was highest at pH 7.5 with 0.5 mg/L NaCl concentration and 0.5 g/L of NaNO(3) as a nitrogen source. This currently unidentified microalga flourished in the presence of diesel, with maximum algal cell numbers on day 7 of incubation in the presence of 1% v/v diesel. Chlorophyll a, b and carotenoid contents of the culture were greatest on day 9 of incubation. The diesel degradation achieved was 64.5% of the original concentration after 9 days. Gas chromatography analysis showed the complete mineralisation of C(7)–C(13) hydrocarbon chains. Fourier transform infrared spectroscopy analysis confirmed that strain WCY_AQ5_3 fully degraded the hydrocarbon with bioabsorption of the products. Morphological and molecular analyses suggested that this spherical, single-celled green microalga was a member of the genus Micractinium. The data obtained confirm that this microalga is a suitable candidate for further research into the degradation of diesel in Antarctica. MDPI 2023-07-03 /pmc/articles/PMC10347017/ /pubmed/37447097 http://dx.doi.org/10.3390/plants12132536 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zamree, Nur Diyanah
Puasa, Nurul Aini
Lim, Zheng Syuen
Wong, Chiew-Yen
Shaharuddin, Noor Azmi
Zakaria, Nur Nadhirah
Merican, Faradina
Convey, Peter
Ahmad, Syahida
Shaari, Hasrizal
Azmi, Alyza Azzura
Ahmad, Siti Aqlima
Zulkharnain, Azham
The Utilisation of Antarctic Microalgae Isolated from Paradise Bay (Antarctic Peninsula) in the Bioremediation of Diesel
title The Utilisation of Antarctic Microalgae Isolated from Paradise Bay (Antarctic Peninsula) in the Bioremediation of Diesel
title_full The Utilisation of Antarctic Microalgae Isolated from Paradise Bay (Antarctic Peninsula) in the Bioremediation of Diesel
title_fullStr The Utilisation of Antarctic Microalgae Isolated from Paradise Bay (Antarctic Peninsula) in the Bioremediation of Diesel
title_full_unstemmed The Utilisation of Antarctic Microalgae Isolated from Paradise Bay (Antarctic Peninsula) in the Bioremediation of Diesel
title_short The Utilisation of Antarctic Microalgae Isolated from Paradise Bay (Antarctic Peninsula) in the Bioremediation of Diesel
title_sort utilisation of antarctic microalgae isolated from paradise bay (antarctic peninsula) in the bioremediation of diesel
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10347017/
https://www.ncbi.nlm.nih.gov/pubmed/37447097
http://dx.doi.org/10.3390/plants12132536
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