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High Temperature Tolerance in a Novel, High-Quality Phaseolus vulgaris Breeding Line Is Due to Maintenance of Pollen Viability and Successful Germination on the Stigma

The common bean (Phaseolus vulgaris L.) is an important nutritional source globally but is sensitive to high temperatures and thus particularly vulnerable to climate change. Derived from a breeding program at CIAT (Colombia), a heat-tolerant breeding line, named heat-tolerant Andean-type 4 (HTA4), w...

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Autores principales: Rose, Teresa, Lowe, Claudia, Miret, Javier A., Walpole, Hannah, Halsey, Kirstie, Venter, Eudri, Urban, Milan O., Buendia, Hector Fabio, Kurup, Smita, O’Sullivan, Donal Martin, Beebe, Steve, Heuer, Sigrid
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10347029/
https://www.ncbi.nlm.nih.gov/pubmed/37447054
http://dx.doi.org/10.3390/plants12132491
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author Rose, Teresa
Lowe, Claudia
Miret, Javier A.
Walpole, Hannah
Halsey, Kirstie
Venter, Eudri
Urban, Milan O.
Buendia, Hector Fabio
Kurup, Smita
O’Sullivan, Donal Martin
Beebe, Steve
Heuer, Sigrid
author_facet Rose, Teresa
Lowe, Claudia
Miret, Javier A.
Walpole, Hannah
Halsey, Kirstie
Venter, Eudri
Urban, Milan O.
Buendia, Hector Fabio
Kurup, Smita
O’Sullivan, Donal Martin
Beebe, Steve
Heuer, Sigrid
author_sort Rose, Teresa
collection PubMed
description The common bean (Phaseolus vulgaris L.) is an important nutritional source globally but is sensitive to high temperatures and thus particularly vulnerable to climate change. Derived from a breeding program at CIAT (Colombia), a heat-tolerant breeding line, named heat-tolerant Andean-type 4 (HTA4), was developed by a series of crosses of parents with a small-bean tepary genotype (Phaseolus acutifolius L.) in their pedigree, which might be the donor of heat stress (HS) tolerance. Importantly, in HTA4, the large, commercially desirable Andean-type beans was restored. To assess underlying tolerance mechanisms, HTA4, together with a heat-sensitive Colombian variety (Calima), was exposed to HS (31 °C/24 °C HS vs. 26 °C/19 °C day/night) under controlled environment conditions. Vegetative growth and photosynthetic performance were not negatively impacted by HS in either genotype, although senescence was delayed in Calima. HS during the reproductive stage caused an increase in pod number in Calima but with few fully developed seeds and many pods aborted and/or abscised. In contrast, HTA4 maintained a similar filled pod number under HS and a higher seed weight per plant. Pollen showed high sterility in Calima, with many non-viable pollen grains (24.9% viability compared to 98.4% in control) with a thicker exine and fewer starch granules under HS. Calima pollen failed to adhere to the stigma and germinate under HS. In HTA4, pollen viability was significantly higher than in Calima (71.1% viability compared to 95.4% under control), and pollen successfully germinated and formed pollen tubes in the style under HS. It is concluded that HTA4 is heat tolerant and maintains a high level of reproductive output due to its ability to produce healthy pollen that is able to adhere to the stigma.
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spelling pubmed-103470292023-07-15 High Temperature Tolerance in a Novel, High-Quality Phaseolus vulgaris Breeding Line Is Due to Maintenance of Pollen Viability and Successful Germination on the Stigma Rose, Teresa Lowe, Claudia Miret, Javier A. Walpole, Hannah Halsey, Kirstie Venter, Eudri Urban, Milan O. Buendia, Hector Fabio Kurup, Smita O’Sullivan, Donal Martin Beebe, Steve Heuer, Sigrid Plants (Basel) Article The common bean (Phaseolus vulgaris L.) is an important nutritional source globally but is sensitive to high temperatures and thus particularly vulnerable to climate change. Derived from a breeding program at CIAT (Colombia), a heat-tolerant breeding line, named heat-tolerant Andean-type 4 (HTA4), was developed by a series of crosses of parents with a small-bean tepary genotype (Phaseolus acutifolius L.) in their pedigree, which might be the donor of heat stress (HS) tolerance. Importantly, in HTA4, the large, commercially desirable Andean-type beans was restored. To assess underlying tolerance mechanisms, HTA4, together with a heat-sensitive Colombian variety (Calima), was exposed to HS (31 °C/24 °C HS vs. 26 °C/19 °C day/night) under controlled environment conditions. Vegetative growth and photosynthetic performance were not negatively impacted by HS in either genotype, although senescence was delayed in Calima. HS during the reproductive stage caused an increase in pod number in Calima but with few fully developed seeds and many pods aborted and/or abscised. In contrast, HTA4 maintained a similar filled pod number under HS and a higher seed weight per plant. Pollen showed high sterility in Calima, with many non-viable pollen grains (24.9% viability compared to 98.4% in control) with a thicker exine and fewer starch granules under HS. Calima pollen failed to adhere to the stigma and germinate under HS. In HTA4, pollen viability was significantly higher than in Calima (71.1% viability compared to 95.4% under control), and pollen successfully germinated and formed pollen tubes in the style under HS. It is concluded that HTA4 is heat tolerant and maintains a high level of reproductive output due to its ability to produce healthy pollen that is able to adhere to the stigma. MDPI 2023-06-29 /pmc/articles/PMC10347029/ /pubmed/37447054 http://dx.doi.org/10.3390/plants12132491 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rose, Teresa
Lowe, Claudia
Miret, Javier A.
Walpole, Hannah
Halsey, Kirstie
Venter, Eudri
Urban, Milan O.
Buendia, Hector Fabio
Kurup, Smita
O’Sullivan, Donal Martin
Beebe, Steve
Heuer, Sigrid
High Temperature Tolerance in a Novel, High-Quality Phaseolus vulgaris Breeding Line Is Due to Maintenance of Pollen Viability and Successful Germination on the Stigma
title High Temperature Tolerance in a Novel, High-Quality Phaseolus vulgaris Breeding Line Is Due to Maintenance of Pollen Viability and Successful Germination on the Stigma
title_full High Temperature Tolerance in a Novel, High-Quality Phaseolus vulgaris Breeding Line Is Due to Maintenance of Pollen Viability and Successful Germination on the Stigma
title_fullStr High Temperature Tolerance in a Novel, High-Quality Phaseolus vulgaris Breeding Line Is Due to Maintenance of Pollen Viability and Successful Germination on the Stigma
title_full_unstemmed High Temperature Tolerance in a Novel, High-Quality Phaseolus vulgaris Breeding Line Is Due to Maintenance of Pollen Viability and Successful Germination on the Stigma
title_short High Temperature Tolerance in a Novel, High-Quality Phaseolus vulgaris Breeding Line Is Due to Maintenance of Pollen Viability and Successful Germination on the Stigma
title_sort high temperature tolerance in a novel, high-quality phaseolus vulgaris breeding line is due to maintenance of pollen viability and successful germination on the stigma
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10347029/
https://www.ncbi.nlm.nih.gov/pubmed/37447054
http://dx.doi.org/10.3390/plants12132491
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