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Strongly Emitting Folic Acid-Derived Carbon Nanodots for One- and Two-Photon Imaging of Lyotropic Myelin Figures

[Image: see text] Non-invasive imaging of morphological changes in biologically relevant lipidic mesophases is essential for the understanding of membrane-mediated processes. However, its methodological aspects need to be further explored, with particular attention paid to the design of new excellen...

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Autores principales: Benkowska-Biernacka, Dominika, Mucha, Sebastian G., Firlej, Lucyna, Formalik, Filip, Bantignies, Jean-Louis, Anglaret, Eric, Samoć, Marek, Matczyszyn, Katarzyna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2023
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10347123/
https://www.ncbi.nlm.nih.gov/pubmed/37366586
http://dx.doi.org/10.1021/acsami.3c05656
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author Benkowska-Biernacka, Dominika
Mucha, Sebastian G.
Firlej, Lucyna
Formalik, Filip
Bantignies, Jean-Louis
Anglaret, Eric
Samoć, Marek
Matczyszyn, Katarzyna
author_facet Benkowska-Biernacka, Dominika
Mucha, Sebastian G.
Firlej, Lucyna
Formalik, Filip
Bantignies, Jean-Louis
Anglaret, Eric
Samoć, Marek
Matczyszyn, Katarzyna
author_sort Benkowska-Biernacka, Dominika
collection PubMed
description [Image: see text] Non-invasive imaging of morphological changes in biologically relevant lipidic mesophases is essential for the understanding of membrane-mediated processes. However, its methodological aspects need to be further explored, with particular attention paid to the design of new excellent fluorescent probes. Here, we have demonstrated that bright and biocompatible folic acid-derived carbon nanodots (FA CNDs) may be successfully applied as fluorescent markers in one- and two-photon imaging of bioinspired myelin figures (MFs). Structural and optical properties of these new FA CNDs were first extensively characterized; they revealed remarkable fluorescence performance in linear and non-linear excitation regimes, justifying further applications. Then, confocal fluorescence microscopy and two-photon excited fluorescence microscopy were used to investigate a three-dimensional distribution of FA CNDs within the phospholipid-based MFs. Our results showed that FA CNDs are effective markers for imaging various forms and parts of multilamellar microstructures.
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spelling pubmed-103471232023-07-15 Strongly Emitting Folic Acid-Derived Carbon Nanodots for One- and Two-Photon Imaging of Lyotropic Myelin Figures Benkowska-Biernacka, Dominika Mucha, Sebastian G. Firlej, Lucyna Formalik, Filip Bantignies, Jean-Louis Anglaret, Eric Samoć, Marek Matczyszyn, Katarzyna ACS Appl Mater Interfaces [Image: see text] Non-invasive imaging of morphological changes in biologically relevant lipidic mesophases is essential for the understanding of membrane-mediated processes. However, its methodological aspects need to be further explored, with particular attention paid to the design of new excellent fluorescent probes. Here, we have demonstrated that bright and biocompatible folic acid-derived carbon nanodots (FA CNDs) may be successfully applied as fluorescent markers in one- and two-photon imaging of bioinspired myelin figures (MFs). Structural and optical properties of these new FA CNDs were first extensively characterized; they revealed remarkable fluorescence performance in linear and non-linear excitation regimes, justifying further applications. Then, confocal fluorescence microscopy and two-photon excited fluorescence microscopy were used to investigate a three-dimensional distribution of FA CNDs within the phospholipid-based MFs. Our results showed that FA CNDs are effective markers for imaging various forms and parts of multilamellar microstructures. American Chemical Society 2023-06-27 /pmc/articles/PMC10347123/ /pubmed/37366586 http://dx.doi.org/10.1021/acsami.3c05656 Text en © 2023 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Benkowska-Biernacka, Dominika
Mucha, Sebastian G.
Firlej, Lucyna
Formalik, Filip
Bantignies, Jean-Louis
Anglaret, Eric
Samoć, Marek
Matczyszyn, Katarzyna
Strongly Emitting Folic Acid-Derived Carbon Nanodots for One- and Two-Photon Imaging of Lyotropic Myelin Figures
title Strongly Emitting Folic Acid-Derived Carbon Nanodots for One- and Two-Photon Imaging of Lyotropic Myelin Figures
title_full Strongly Emitting Folic Acid-Derived Carbon Nanodots for One- and Two-Photon Imaging of Lyotropic Myelin Figures
title_fullStr Strongly Emitting Folic Acid-Derived Carbon Nanodots for One- and Two-Photon Imaging of Lyotropic Myelin Figures
title_full_unstemmed Strongly Emitting Folic Acid-Derived Carbon Nanodots for One- and Two-Photon Imaging of Lyotropic Myelin Figures
title_short Strongly Emitting Folic Acid-Derived Carbon Nanodots for One- and Two-Photon Imaging of Lyotropic Myelin Figures
title_sort strongly emitting folic acid-derived carbon nanodots for one- and two-photon imaging of lyotropic myelin figures
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10347123/
https://www.ncbi.nlm.nih.gov/pubmed/37366586
http://dx.doi.org/10.1021/acsami.3c05656
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