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Films of Poly(Hydroxybutyrate) (PHB) and Copper with Antibacterial Activity

Poly(3-hydroxybutyrate), PHB, is a hydrophobic biopolymer with good mechanical and barrier properties. However, neat PHB is a semicrystalline polymer with a relative high degree of crystallinity and poor film properties. In this work, this biopolymer was plasticized with glycerol tributyrate and fun...

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Detalles Bibliográficos
Autores principales: Quispe, Mayte M., Villanueva, María E., Copello, Guillermo J., López, Olivia V., Villar, Marcelo A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10347170/
https://www.ncbi.nlm.nih.gov/pubmed/37447552
http://dx.doi.org/10.3390/polym15132907
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author Quispe, Mayte M.
Villanueva, María E.
Copello, Guillermo J.
López, Olivia V.
Villar, Marcelo A.
author_facet Quispe, Mayte M.
Villanueva, María E.
Copello, Guillermo J.
López, Olivia V.
Villar, Marcelo A.
author_sort Quispe, Mayte M.
collection PubMed
description Poly(3-hydroxybutyrate), PHB, is a hydrophobic biopolymer with good mechanical and barrier properties. However, neat PHB is a semicrystalline polymer with a relative high degree of crystallinity and poor film properties. In this work, this biopolymer was plasticized with glycerol tributyrate and functionalized with copper (II) sulfate, allowing us to obtain biodegradable antimicrobial flexible films. Films with the minimum inhibitory concentration (MIC) of copper (II) sulfate presented a higher roughness than neat PHB films. The presence of plasticizer significantly improved the copper sulfate diffusion process, which was evidenced by a greater inhibition halo for plasticized materials compared to unplasticized ones, at the same salt concentration. Plasticized PHB with 2.5% copper (II) sulfate inhibited both Gram-positive (Staphylococcus aureus) and Gram-negative (Pseudomona aeruginosa) bacteria, as determined by the bacterial inhibition halo. In addition, neat PHB films and PHB containing copper (II) sulfate did not show in vitro cytotoxicity in the L-929 cell line. Thus, plasticized PHB functionalized with copper (II) sulfate can be used as biodegradable antimicrobial flexible films for different applications.
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spelling pubmed-103471702023-07-15 Films of Poly(Hydroxybutyrate) (PHB) and Copper with Antibacterial Activity Quispe, Mayte M. Villanueva, María E. Copello, Guillermo J. López, Olivia V. Villar, Marcelo A. Polymers (Basel) Article Poly(3-hydroxybutyrate), PHB, is a hydrophobic biopolymer with good mechanical and barrier properties. However, neat PHB is a semicrystalline polymer with a relative high degree of crystallinity and poor film properties. In this work, this biopolymer was plasticized with glycerol tributyrate and functionalized with copper (II) sulfate, allowing us to obtain biodegradable antimicrobial flexible films. Films with the minimum inhibitory concentration (MIC) of copper (II) sulfate presented a higher roughness than neat PHB films. The presence of plasticizer significantly improved the copper sulfate diffusion process, which was evidenced by a greater inhibition halo for plasticized materials compared to unplasticized ones, at the same salt concentration. Plasticized PHB with 2.5% copper (II) sulfate inhibited both Gram-positive (Staphylococcus aureus) and Gram-negative (Pseudomona aeruginosa) bacteria, as determined by the bacterial inhibition halo. In addition, neat PHB films and PHB containing copper (II) sulfate did not show in vitro cytotoxicity in the L-929 cell line. Thus, plasticized PHB functionalized with copper (II) sulfate can be used as biodegradable antimicrobial flexible films for different applications. MDPI 2023-06-30 /pmc/articles/PMC10347170/ /pubmed/37447552 http://dx.doi.org/10.3390/polym15132907 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Quispe, Mayte M.
Villanueva, María E.
Copello, Guillermo J.
López, Olivia V.
Villar, Marcelo A.
Films of Poly(Hydroxybutyrate) (PHB) and Copper with Antibacterial Activity
title Films of Poly(Hydroxybutyrate) (PHB) and Copper with Antibacterial Activity
title_full Films of Poly(Hydroxybutyrate) (PHB) and Copper with Antibacterial Activity
title_fullStr Films of Poly(Hydroxybutyrate) (PHB) and Copper with Antibacterial Activity
title_full_unstemmed Films of Poly(Hydroxybutyrate) (PHB) and Copper with Antibacterial Activity
title_short Films of Poly(Hydroxybutyrate) (PHB) and Copper with Antibacterial Activity
title_sort films of poly(hydroxybutyrate) (phb) and copper with antibacterial activity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10347170/
https://www.ncbi.nlm.nih.gov/pubmed/37447552
http://dx.doi.org/10.3390/polym15132907
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