Cargando…

African swine fever virus protein p17 promotes mitophagy by facilitating the interaction of SQSTM1 with TOMM70

Viruses have developed different strategies to hijack mitophagy to facilitate their replication. However, whether and how African swine fever virus (ASFV) regulates mitophagy are largely unknown. Here, we found that the ASFV-encoded p17 induced mitophagy. Coimmunoprecipitation/mass spectrometry assa...

Descripción completa

Detalles Bibliográficos
Autores principales: Hu, Boli, Zhong, Guifang, Ding, Shuxiang, Xu, Kang, Peng, Xiran, Dong, Weiren, Zhou, Jiyong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10348029/
https://www.ncbi.nlm.nih.gov/pubmed/37442088
http://dx.doi.org/10.1080/21505594.2023.2232707
_version_ 1785073629511286784
author Hu, Boli
Zhong, Guifang
Ding, Shuxiang
Xu, Kang
Peng, Xiran
Dong, Weiren
Zhou, Jiyong
author_facet Hu, Boli
Zhong, Guifang
Ding, Shuxiang
Xu, Kang
Peng, Xiran
Dong, Weiren
Zhou, Jiyong
author_sort Hu, Boli
collection PubMed
description Viruses have developed different strategies to hijack mitophagy to facilitate their replication. However, whether and how African swine fever virus (ASFV) regulates mitophagy are largely unknown. Here, we found that the ASFV-encoded p17 induced mitophagy. Coimmunoprecipitation/mass spectrometry assays identified translocase of outer mitochondrial membrane 70 (TOMM70) as the protein that interacted with p17. The binding of TOMM70 to p17 promoted the binding of the mitophagy receptor SQSTM1 to TOMM70, led to engulfment of mitochondria by autophagosomes, and consequently decreased the number of mitochondria. Consistently, the levels of TOMM70 and TOMM20 decreased substantially after p17 expression or ASFV infection. Furthermore, p17-mediated mitophagy resulted in the degradation of mitochondrial antiviral signalling proteins and inhibited the production of IFN-α, IL-6 and TNFα. Overall, our findings suggest that ASFV p17 regulates innate immunity by inducing mitophagy via the interaction of SQSTM1 with TOMM70.
format Online
Article
Text
id pubmed-10348029
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Taylor & Francis
record_format MEDLINE/PubMed
spelling pubmed-103480292023-07-15 African swine fever virus protein p17 promotes mitophagy by facilitating the interaction of SQSTM1 with TOMM70 Hu, Boli Zhong, Guifang Ding, Shuxiang Xu, Kang Peng, Xiran Dong, Weiren Zhou, Jiyong Virulence Research Paper Viruses have developed different strategies to hijack mitophagy to facilitate their replication. However, whether and how African swine fever virus (ASFV) regulates mitophagy are largely unknown. Here, we found that the ASFV-encoded p17 induced mitophagy. Coimmunoprecipitation/mass spectrometry assays identified translocase of outer mitochondrial membrane 70 (TOMM70) as the protein that interacted with p17. The binding of TOMM70 to p17 promoted the binding of the mitophagy receptor SQSTM1 to TOMM70, led to engulfment of mitochondria by autophagosomes, and consequently decreased the number of mitochondria. Consistently, the levels of TOMM70 and TOMM20 decreased substantially after p17 expression or ASFV infection. Furthermore, p17-mediated mitophagy resulted in the degradation of mitochondrial antiviral signalling proteins and inhibited the production of IFN-α, IL-6 and TNFα. Overall, our findings suggest that ASFV p17 regulates innate immunity by inducing mitophagy via the interaction of SQSTM1 with TOMM70. Taylor & Francis 2023-07-13 /pmc/articles/PMC10348029/ /pubmed/37442088 http://dx.doi.org/10.1080/21505594.2023.2232707 Text en © 2023 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent.
spellingShingle Research Paper
Hu, Boli
Zhong, Guifang
Ding, Shuxiang
Xu, Kang
Peng, Xiran
Dong, Weiren
Zhou, Jiyong
African swine fever virus protein p17 promotes mitophagy by facilitating the interaction of SQSTM1 with TOMM70
title African swine fever virus protein p17 promotes mitophagy by facilitating the interaction of SQSTM1 with TOMM70
title_full African swine fever virus protein p17 promotes mitophagy by facilitating the interaction of SQSTM1 with TOMM70
title_fullStr African swine fever virus protein p17 promotes mitophagy by facilitating the interaction of SQSTM1 with TOMM70
title_full_unstemmed African swine fever virus protein p17 promotes mitophagy by facilitating the interaction of SQSTM1 with TOMM70
title_short African swine fever virus protein p17 promotes mitophagy by facilitating the interaction of SQSTM1 with TOMM70
title_sort african swine fever virus protein p17 promotes mitophagy by facilitating the interaction of sqstm1 with tomm70
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10348029/
https://www.ncbi.nlm.nih.gov/pubmed/37442088
http://dx.doi.org/10.1080/21505594.2023.2232707
work_keys_str_mv AT huboli africanswinefevervirusproteinp17promotesmitophagybyfacilitatingtheinteractionofsqstm1withtomm70
AT zhongguifang africanswinefevervirusproteinp17promotesmitophagybyfacilitatingtheinteractionofsqstm1withtomm70
AT dingshuxiang africanswinefevervirusproteinp17promotesmitophagybyfacilitatingtheinteractionofsqstm1withtomm70
AT xukang africanswinefevervirusproteinp17promotesmitophagybyfacilitatingtheinteractionofsqstm1withtomm70
AT pengxiran africanswinefevervirusproteinp17promotesmitophagybyfacilitatingtheinteractionofsqstm1withtomm70
AT dongweiren africanswinefevervirusproteinp17promotesmitophagybyfacilitatingtheinteractionofsqstm1withtomm70
AT zhoujiyong africanswinefevervirusproteinp17promotesmitophagybyfacilitatingtheinteractionofsqstm1withtomm70