Cargando…
African swine fever virus protein p17 promotes mitophagy by facilitating the interaction of SQSTM1 with TOMM70
Viruses have developed different strategies to hijack mitophagy to facilitate their replication. However, whether and how African swine fever virus (ASFV) regulates mitophagy are largely unknown. Here, we found that the ASFV-encoded p17 induced mitophagy. Coimmunoprecipitation/mass spectrometry assa...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10348029/ https://www.ncbi.nlm.nih.gov/pubmed/37442088 http://dx.doi.org/10.1080/21505594.2023.2232707 |
_version_ | 1785073629511286784 |
---|---|
author | Hu, Boli Zhong, Guifang Ding, Shuxiang Xu, Kang Peng, Xiran Dong, Weiren Zhou, Jiyong |
author_facet | Hu, Boli Zhong, Guifang Ding, Shuxiang Xu, Kang Peng, Xiran Dong, Weiren Zhou, Jiyong |
author_sort | Hu, Boli |
collection | PubMed |
description | Viruses have developed different strategies to hijack mitophagy to facilitate their replication. However, whether and how African swine fever virus (ASFV) regulates mitophagy are largely unknown. Here, we found that the ASFV-encoded p17 induced mitophagy. Coimmunoprecipitation/mass spectrometry assays identified translocase of outer mitochondrial membrane 70 (TOMM70) as the protein that interacted with p17. The binding of TOMM70 to p17 promoted the binding of the mitophagy receptor SQSTM1 to TOMM70, led to engulfment of mitochondria by autophagosomes, and consequently decreased the number of mitochondria. Consistently, the levels of TOMM70 and TOMM20 decreased substantially after p17 expression or ASFV infection. Furthermore, p17-mediated mitophagy resulted in the degradation of mitochondrial antiviral signalling proteins and inhibited the production of IFN-α, IL-6 and TNFα. Overall, our findings suggest that ASFV p17 regulates innate immunity by inducing mitophagy via the interaction of SQSTM1 with TOMM70. |
format | Online Article Text |
id | pubmed-10348029 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-103480292023-07-15 African swine fever virus protein p17 promotes mitophagy by facilitating the interaction of SQSTM1 with TOMM70 Hu, Boli Zhong, Guifang Ding, Shuxiang Xu, Kang Peng, Xiran Dong, Weiren Zhou, Jiyong Virulence Research Paper Viruses have developed different strategies to hijack mitophagy to facilitate their replication. However, whether and how African swine fever virus (ASFV) regulates mitophagy are largely unknown. Here, we found that the ASFV-encoded p17 induced mitophagy. Coimmunoprecipitation/mass spectrometry assays identified translocase of outer mitochondrial membrane 70 (TOMM70) as the protein that interacted with p17. The binding of TOMM70 to p17 promoted the binding of the mitophagy receptor SQSTM1 to TOMM70, led to engulfment of mitochondria by autophagosomes, and consequently decreased the number of mitochondria. Consistently, the levels of TOMM70 and TOMM20 decreased substantially after p17 expression or ASFV infection. Furthermore, p17-mediated mitophagy resulted in the degradation of mitochondrial antiviral signalling proteins and inhibited the production of IFN-α, IL-6 and TNFα. Overall, our findings suggest that ASFV p17 regulates innate immunity by inducing mitophagy via the interaction of SQSTM1 with TOMM70. Taylor & Francis 2023-07-13 /pmc/articles/PMC10348029/ /pubmed/37442088 http://dx.doi.org/10.1080/21505594.2023.2232707 Text en © 2023 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent. |
spellingShingle | Research Paper Hu, Boli Zhong, Guifang Ding, Shuxiang Xu, Kang Peng, Xiran Dong, Weiren Zhou, Jiyong African swine fever virus protein p17 promotes mitophagy by facilitating the interaction of SQSTM1 with TOMM70 |
title | African swine fever virus protein p17 promotes mitophagy by facilitating the interaction of SQSTM1 with TOMM70 |
title_full | African swine fever virus protein p17 promotes mitophagy by facilitating the interaction of SQSTM1 with TOMM70 |
title_fullStr | African swine fever virus protein p17 promotes mitophagy by facilitating the interaction of SQSTM1 with TOMM70 |
title_full_unstemmed | African swine fever virus protein p17 promotes mitophagy by facilitating the interaction of SQSTM1 with TOMM70 |
title_short | African swine fever virus protein p17 promotes mitophagy by facilitating the interaction of SQSTM1 with TOMM70 |
title_sort | african swine fever virus protein p17 promotes mitophagy by facilitating the interaction of sqstm1 with tomm70 |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10348029/ https://www.ncbi.nlm.nih.gov/pubmed/37442088 http://dx.doi.org/10.1080/21505594.2023.2232707 |
work_keys_str_mv | AT huboli africanswinefevervirusproteinp17promotesmitophagybyfacilitatingtheinteractionofsqstm1withtomm70 AT zhongguifang africanswinefevervirusproteinp17promotesmitophagybyfacilitatingtheinteractionofsqstm1withtomm70 AT dingshuxiang africanswinefevervirusproteinp17promotesmitophagybyfacilitatingtheinteractionofsqstm1withtomm70 AT xukang africanswinefevervirusproteinp17promotesmitophagybyfacilitatingtheinteractionofsqstm1withtomm70 AT pengxiran africanswinefevervirusproteinp17promotesmitophagybyfacilitatingtheinteractionofsqstm1withtomm70 AT dongweiren africanswinefevervirusproteinp17promotesmitophagybyfacilitatingtheinteractionofsqstm1withtomm70 AT zhoujiyong africanswinefevervirusproteinp17promotesmitophagybyfacilitatingtheinteractionofsqstm1withtomm70 |