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Fabrication of impedimetric sensor based on metallic nanoparticle for the determination of mesna anticancer drug
Electrochemical impedance spectroscopy (EIS) is a highly effective technique for studying the surface of electrodes in great detail. EIS-based electrochemical sensors have been widely reported, which measure the charge transfer resistance (R(ct)) of redox probes on electrode surfaces to monitor the...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10349103/ https://www.ncbi.nlm.nih.gov/pubmed/37452101 http://dx.doi.org/10.1038/s41598-023-38643-3 |
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author | Mehrban, Maryam Madrakian, Tayyebeh Afkhami, Abbas Jalal, Nahid Rezvani |
author_facet | Mehrban, Maryam Madrakian, Tayyebeh Afkhami, Abbas Jalal, Nahid Rezvani |
author_sort | Mehrban, Maryam |
collection | PubMed |
description | Electrochemical impedance spectroscopy (EIS) is a highly effective technique for studying the surface of electrodes in great detail. EIS-based electrochemical sensors have been widely reported, which measure the charge transfer resistance (R(ct)) of redox probes on electrode surfaces to monitor the binding of target molecules. One of the protective drugs against hemorrhagic cystitis caused by oxazaphosphorine chemotherapy drugs such as ifosfamide, cyclophosphamide and trophosphamide is Mesna (sodium salt of 2-mercaptoethanesulfonate). The increase in the use of Mesna due to the high consumption of anti-cancer drugs, the determination of this drug in biological samples is of particular importance. So far, no electrochemical method has been reported to measure Mesna. In this research, a novel impedimetric sensor based on a glassy carbon electrode (GCE) modified with oxidized multiwalled carbon nanotubes (MWCNTs)/gold nanoparticle (AuNPs) (denoted as Au NPs/MWCNTs/GCE) for impedimetric determination of Mesna anticancer drug was developed. The modified electrode materials were characterized by field emission scanning electron microscopy (FESEM), energy dispersive X-ray (EDX), and EIS. The electrochemical behavior of Mesna at the surface of Au NPs/MWCNTs/GCE was studied by an impedimetric method. The detection mechanism of Mesna using the proposed impedimetric sensor relied on the increase in the R(ct) value of [Fe (CN)(6)](3−/4−) as an electrochemical probe in the presence of Mesna compared to the absence of Mesna as the analyte. Under the optimum condition, which covered two linear dynamic ranges from 0.06 nmol L(−1) to 1.0 nmol L(−1) and 1.0 nmol L(−1) to 130.0 µmol L(−1), respectively. The detection limit was 0.02 nmol L(−1). Finally, the performance of the proposed sensor was investigated for Mesna electrochemical detection in biological samples. |
format | Online Article Text |
id | pubmed-10349103 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-103491032023-07-16 Fabrication of impedimetric sensor based on metallic nanoparticle for the determination of mesna anticancer drug Mehrban, Maryam Madrakian, Tayyebeh Afkhami, Abbas Jalal, Nahid Rezvani Sci Rep Article Electrochemical impedance spectroscopy (EIS) is a highly effective technique for studying the surface of electrodes in great detail. EIS-based electrochemical sensors have been widely reported, which measure the charge transfer resistance (R(ct)) of redox probes on electrode surfaces to monitor the binding of target molecules. One of the protective drugs against hemorrhagic cystitis caused by oxazaphosphorine chemotherapy drugs such as ifosfamide, cyclophosphamide and trophosphamide is Mesna (sodium salt of 2-mercaptoethanesulfonate). The increase in the use of Mesna due to the high consumption of anti-cancer drugs, the determination of this drug in biological samples is of particular importance. So far, no electrochemical method has been reported to measure Mesna. In this research, a novel impedimetric sensor based on a glassy carbon electrode (GCE) modified with oxidized multiwalled carbon nanotubes (MWCNTs)/gold nanoparticle (AuNPs) (denoted as Au NPs/MWCNTs/GCE) for impedimetric determination of Mesna anticancer drug was developed. The modified electrode materials were characterized by field emission scanning electron microscopy (FESEM), energy dispersive X-ray (EDX), and EIS. The electrochemical behavior of Mesna at the surface of Au NPs/MWCNTs/GCE was studied by an impedimetric method. The detection mechanism of Mesna using the proposed impedimetric sensor relied on the increase in the R(ct) value of [Fe (CN)(6)](3−/4−) as an electrochemical probe in the presence of Mesna compared to the absence of Mesna as the analyte. Under the optimum condition, which covered two linear dynamic ranges from 0.06 nmol L(−1) to 1.0 nmol L(−1) and 1.0 nmol L(−1) to 130.0 µmol L(−1), respectively. The detection limit was 0.02 nmol L(−1). Finally, the performance of the proposed sensor was investigated for Mesna electrochemical detection in biological samples. Nature Publishing Group UK 2023-07-14 /pmc/articles/PMC10349103/ /pubmed/37452101 http://dx.doi.org/10.1038/s41598-023-38643-3 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Mehrban, Maryam Madrakian, Tayyebeh Afkhami, Abbas Jalal, Nahid Rezvani Fabrication of impedimetric sensor based on metallic nanoparticle for the determination of mesna anticancer drug |
title | Fabrication of impedimetric sensor based on metallic nanoparticle for the determination of mesna anticancer drug |
title_full | Fabrication of impedimetric sensor based on metallic nanoparticle for the determination of mesna anticancer drug |
title_fullStr | Fabrication of impedimetric sensor based on metallic nanoparticle for the determination of mesna anticancer drug |
title_full_unstemmed | Fabrication of impedimetric sensor based on metallic nanoparticle for the determination of mesna anticancer drug |
title_short | Fabrication of impedimetric sensor based on metallic nanoparticle for the determination of mesna anticancer drug |
title_sort | fabrication of impedimetric sensor based on metallic nanoparticle for the determination of mesna anticancer drug |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10349103/ https://www.ncbi.nlm.nih.gov/pubmed/37452101 http://dx.doi.org/10.1038/s41598-023-38643-3 |
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