Modulation of radiosensitivity of DU145 prostate carcinoma cells by simvastatin

PURPOSE: To investigate antiproliferative effects of simvastatin in combination with ionizing radiation on DU145 prostate cancer cells and its influence on cellular HMG-CoA-reductase levels. METHODS: Proliferative responses of DU145 cells were estimated by means of a clonogenic assay or the crystal...

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Detalles Bibliográficos
Autores principales: Korte, Verena, Gademann, Guenther, Gawish, Ahmed, Ochel, Hans-Joachim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2022
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10349728/
https://www.ncbi.nlm.nih.gov/pubmed/36127484
http://dx.doi.org/10.1007/s00432-022-04364-9
Descripción
Sumario:PURPOSE: To investigate antiproliferative effects of simvastatin in combination with ionizing radiation on DU145 prostate cancer cells and its influence on cellular HMG-CoA-reductase levels. METHODS: Proliferative responses of DU145 cells were estimated by means of a clonogenic assay or the crystal violet procedure. HMG-CoA-reductase levels were measured by western blot analysis. RESULTS: The antiproliferative effects of simvastatin and radiation are dependent on simvastatin dose, radiation dose and treatment time. In vitro treatment of DU145 cells with simvastatin induced HMG-CoA-reductase levels. CONCLUSION: Ionizing radiation more profoundly reduces proliferation as compared to simvastatin exposure, while the combined application of both modalities is synergistic. The inhibition of CoA-reductase may contribute to these effects.

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