Modulation of radiosensitivity of DU145 prostate carcinoma cells by simvastatin

PURPOSE: To investigate antiproliferative effects of simvastatin in combination with ionizing radiation on DU145 prostate cancer cells and its influence on cellular HMG-CoA-reductase levels. METHODS: Proliferative responses of DU145 cells were estimated by means of a clonogenic assay or the crystal...

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Autores principales: Korte, Verena, Gademann, Guenther, Gawish, Ahmed, Ochel, Hans-Joachim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2022
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10349728/
https://www.ncbi.nlm.nih.gov/pubmed/36127484
http://dx.doi.org/10.1007/s00432-022-04364-9
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author Korte, Verena
Gademann, Guenther
Gawish, Ahmed
Ochel, Hans-Joachim
author_facet Korte, Verena
Gademann, Guenther
Gawish, Ahmed
Ochel, Hans-Joachim
author_sort Korte, Verena
collection PubMed
description PURPOSE: To investigate antiproliferative effects of simvastatin in combination with ionizing radiation on DU145 prostate cancer cells and its influence on cellular HMG-CoA-reductase levels. METHODS: Proliferative responses of DU145 cells were estimated by means of a clonogenic assay or the crystal violet procedure. HMG-CoA-reductase levels were measured by western blot analysis. RESULTS: The antiproliferative effects of simvastatin and radiation are dependent on simvastatin dose, radiation dose and treatment time. In vitro treatment of DU145 cells with simvastatin induced HMG-CoA-reductase levels. CONCLUSION: Ionizing radiation more profoundly reduces proliferation as compared to simvastatin exposure, while the combined application of both modalities is synergistic. The inhibition of CoA-reductase may contribute to these effects.
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spelling pubmed-103497282023-07-17 Modulation of radiosensitivity of DU145 prostate carcinoma cells by simvastatin Korte, Verena Gademann, Guenther Gawish, Ahmed Ochel, Hans-Joachim J Cancer Res Clin Oncol Research PURPOSE: To investigate antiproliferative effects of simvastatin in combination with ionizing radiation on DU145 prostate cancer cells and its influence on cellular HMG-CoA-reductase levels. METHODS: Proliferative responses of DU145 cells were estimated by means of a clonogenic assay or the crystal violet procedure. HMG-CoA-reductase levels were measured by western blot analysis. RESULTS: The antiproliferative effects of simvastatin and radiation are dependent on simvastatin dose, radiation dose and treatment time. In vitro treatment of DU145 cells with simvastatin induced HMG-CoA-reductase levels. CONCLUSION: Ionizing radiation more profoundly reduces proliferation as compared to simvastatin exposure, while the combined application of both modalities is synergistic. The inhibition of CoA-reductase may contribute to these effects. Springer Berlin Heidelberg 2022-09-21 2023 /pmc/articles/PMC10349728/ /pubmed/36127484 http://dx.doi.org/10.1007/s00432-022-04364-9 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research
Korte, Verena
Gademann, Guenther
Gawish, Ahmed
Ochel, Hans-Joachim
Modulation of radiosensitivity of DU145 prostate carcinoma cells by simvastatin
title Modulation of radiosensitivity of DU145 prostate carcinoma cells by simvastatin
title_full Modulation of radiosensitivity of DU145 prostate carcinoma cells by simvastatin
title_fullStr Modulation of radiosensitivity of DU145 prostate carcinoma cells by simvastatin
title_full_unstemmed Modulation of radiosensitivity of DU145 prostate carcinoma cells by simvastatin
title_short Modulation of radiosensitivity of DU145 prostate carcinoma cells by simvastatin
title_sort modulation of radiosensitivity of du145 prostate carcinoma cells by simvastatin
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10349728/
https://www.ncbi.nlm.nih.gov/pubmed/36127484
http://dx.doi.org/10.1007/s00432-022-04364-9
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