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Cooperative microbial interactions drive spatial segregation in porous environments
The role of microbial interactions and the underlying mechanisms that shape complex biofilm communities are poorly understood. Here we employ a microfluidic chip to represent porous subsurface environments and show that cooperative microbial interactions between free-living and biofilm-forming bacte...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10349867/ https://www.ncbi.nlm.nih.gov/pubmed/37454222 http://dx.doi.org/10.1038/s41467-023-39991-4 |
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author | Wu, Yichao Fu, Chengxia Peacock, Caroline L. Sørensen, Søren J. Redmile-Gordon, Marc A. Xiao, Ke-Qing Gao, Chunhui Liu, Jun Huang, Qiaoyun Li, Zixue Song, Peiyi Zhu, Yongguan Zhou, Jizhong Cai, Peng |
author_facet | Wu, Yichao Fu, Chengxia Peacock, Caroline L. Sørensen, Søren J. Redmile-Gordon, Marc A. Xiao, Ke-Qing Gao, Chunhui Liu, Jun Huang, Qiaoyun Li, Zixue Song, Peiyi Zhu, Yongguan Zhou, Jizhong Cai, Peng |
author_sort | Wu, Yichao |
collection | PubMed |
description | The role of microbial interactions and the underlying mechanisms that shape complex biofilm communities are poorly understood. Here we employ a microfluidic chip to represent porous subsurface environments and show that cooperative microbial interactions between free-living and biofilm-forming bacteria trigger active spatial segregation to promote their respective dominance in segregated microhabitats. During initial colonization, free-living and biofilm-forming microbes are segregated from the mixed planktonic inoculum to occupy the ambient fluid and grain surface. Contrary to spatial exclusion through competition, the active spatial segregation is induced by cooperative interactions which improves the fitness of both biofilm and planktonic populations. We further show that free-living Arthrobacter induces the surface colonization by scavenging the biofilm inhibitor, D-amino acids and receives benefits from the public goods secreted by the biofilm-forming strains. Collectively, our results reveal how cooperative microbial interactions may contribute to microbial coexistence in segregated microhabitats and drive subsurface biofilm community succession. |
format | Online Article Text |
id | pubmed-10349867 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-103498672023-07-17 Cooperative microbial interactions drive spatial segregation in porous environments Wu, Yichao Fu, Chengxia Peacock, Caroline L. Sørensen, Søren J. Redmile-Gordon, Marc A. Xiao, Ke-Qing Gao, Chunhui Liu, Jun Huang, Qiaoyun Li, Zixue Song, Peiyi Zhu, Yongguan Zhou, Jizhong Cai, Peng Nat Commun Article The role of microbial interactions and the underlying mechanisms that shape complex biofilm communities are poorly understood. Here we employ a microfluidic chip to represent porous subsurface environments and show that cooperative microbial interactions between free-living and biofilm-forming bacteria trigger active spatial segregation to promote their respective dominance in segregated microhabitats. During initial colonization, free-living and biofilm-forming microbes are segregated from the mixed planktonic inoculum to occupy the ambient fluid and grain surface. Contrary to spatial exclusion through competition, the active spatial segregation is induced by cooperative interactions which improves the fitness of both biofilm and planktonic populations. We further show that free-living Arthrobacter induces the surface colonization by scavenging the biofilm inhibitor, D-amino acids and receives benefits from the public goods secreted by the biofilm-forming strains. Collectively, our results reveal how cooperative microbial interactions may contribute to microbial coexistence in segregated microhabitats and drive subsurface biofilm community succession. Nature Publishing Group UK 2023-07-15 /pmc/articles/PMC10349867/ /pubmed/37454222 http://dx.doi.org/10.1038/s41467-023-39991-4 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Wu, Yichao Fu, Chengxia Peacock, Caroline L. Sørensen, Søren J. Redmile-Gordon, Marc A. Xiao, Ke-Qing Gao, Chunhui Liu, Jun Huang, Qiaoyun Li, Zixue Song, Peiyi Zhu, Yongguan Zhou, Jizhong Cai, Peng Cooperative microbial interactions drive spatial segregation in porous environments |
title | Cooperative microbial interactions drive spatial segregation in porous environments |
title_full | Cooperative microbial interactions drive spatial segregation in porous environments |
title_fullStr | Cooperative microbial interactions drive spatial segregation in porous environments |
title_full_unstemmed | Cooperative microbial interactions drive spatial segregation in porous environments |
title_short | Cooperative microbial interactions drive spatial segregation in porous environments |
title_sort | cooperative microbial interactions drive spatial segregation in porous environments |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10349867/ https://www.ncbi.nlm.nih.gov/pubmed/37454222 http://dx.doi.org/10.1038/s41467-023-39991-4 |
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