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Recyclable CRISPR/Cas9 mediated gene disruption and deletions in Histoplasma

Targeted gene disruption is challenging in the dimorphic fungal pathogen Histoplasma due to the low frequency of homologous recombination. Transformed DNA is either integrated ectopically into the genome or maintained extra chromosomally by de novo addition of telomeric sequences. Based on a system...

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Detalles Bibliográficos
Autores principales:	Joehnk, Bastian, Ali, Nebat, Voorhies, Mark, Walcott, Keith, Sil, Anita
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Cold Spring Harbor Laboratory 2023
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10350005/ https://www.ncbi.nlm.nih.gov/pubmed/37461713 http://dx.doi.org/10.1101/2023.07.05.547774

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