Cargando…

Nuclear envelope assembly relies on CHMP-7 in the absence of BAF-LEM-mediated hole closure

Barrier-to-autointegration factor (BAF) is a DNA binding protein that crosslinks chromatin to assemble the nuclear envelope (NE) after mitosis. BAF also binds the Lap2b-Emerin-Man1 (LEM) domain family of NE proteins to repair interphase ruptures. The NE adaptors to ESCRTs, LEMD2-CHMP7, seal NE holes...

Descripción completa

Detalles Bibliográficos
Autores principales: Barger, Sarah R., Penfield, Lauren, Bahmanyar, Shirin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10350047/
https://www.ncbi.nlm.nih.gov/pubmed/37461528
http://dx.doi.org/10.1101/2023.07.06.547980
_version_ 1785074049951465472
author Barger, Sarah R.
Penfield, Lauren
Bahmanyar, Shirin
author_facet Barger, Sarah R.
Penfield, Lauren
Bahmanyar, Shirin
author_sort Barger, Sarah R.
collection PubMed
description Barrier-to-autointegration factor (BAF) is a DNA binding protein that crosslinks chromatin to assemble the nuclear envelope (NE) after mitosis. BAF also binds the Lap2b-Emerin-Man1 (LEM) domain family of NE proteins to repair interphase ruptures. The NE adaptors to ESCRTs, LEMD2-CHMP7, seal NE holes surrounding mitotic spindle microtubules (MTs), but whether NE hole closure in mitosis involves BAF-LEM binding is not known. Here, we analyze NE sealing after meiosis II in C. elegans oocytes to show that BAF-LEM binding and LEM-2(LEMD2)-CHMP-7 have distinct roles in hole closure around spindle MTs. LEM-2/EMR-1(emerin) function redundantly with BAF-1 to seal the NE. Compromising BAF-LEM binding revealed an additional role for EMR-1 in maintenance of the NE permeability barrier and an essential role for LEM-2-CHMP-7 in preventing NE assembly failure. The WH domain of LEM-2 recruits the majority of CHMP-7 to the NE in C. elegans and a LEM-2 -independent pool of CHMP-7, which is mostly enriched in the nucleoplasm, also contributes to NE stability. Thus, NE hole closure surrounding spindle MTs requires redundant mechanisms that safeguard against failure in NE assembly to support embryogenesis.
format Online
Article
Text
id pubmed-10350047
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Cold Spring Harbor Laboratory
record_format MEDLINE/PubMed
spelling pubmed-103500472023-07-17 Nuclear envelope assembly relies on CHMP-7 in the absence of BAF-LEM-mediated hole closure Barger, Sarah R. Penfield, Lauren Bahmanyar, Shirin bioRxiv Article Barrier-to-autointegration factor (BAF) is a DNA binding protein that crosslinks chromatin to assemble the nuclear envelope (NE) after mitosis. BAF also binds the Lap2b-Emerin-Man1 (LEM) domain family of NE proteins to repair interphase ruptures. The NE adaptors to ESCRTs, LEMD2-CHMP7, seal NE holes surrounding mitotic spindle microtubules (MTs), but whether NE hole closure in mitosis involves BAF-LEM binding is not known. Here, we analyze NE sealing after meiosis II in C. elegans oocytes to show that BAF-LEM binding and LEM-2(LEMD2)-CHMP-7 have distinct roles in hole closure around spindle MTs. LEM-2/EMR-1(emerin) function redundantly with BAF-1 to seal the NE. Compromising BAF-LEM binding revealed an additional role for EMR-1 in maintenance of the NE permeability barrier and an essential role for LEM-2-CHMP-7 in preventing NE assembly failure. The WH domain of LEM-2 recruits the majority of CHMP-7 to the NE in C. elegans and a LEM-2 -independent pool of CHMP-7, which is mostly enriched in the nucleoplasm, also contributes to NE stability. Thus, NE hole closure surrounding spindle MTs requires redundant mechanisms that safeguard against failure in NE assembly to support embryogenesis. Cold Spring Harbor Laboratory 2023-07-07 /pmc/articles/PMC10350047/ /pubmed/37461528 http://dx.doi.org/10.1101/2023.07.06.547980 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which allows reusers to copy and distribute the material in any medium or format in unadapted form only, for noncommercial purposes only, and only so long as attribution is given to the creator.
spellingShingle Article
Barger, Sarah R.
Penfield, Lauren
Bahmanyar, Shirin
Nuclear envelope assembly relies on CHMP-7 in the absence of BAF-LEM-mediated hole closure
title Nuclear envelope assembly relies on CHMP-7 in the absence of BAF-LEM-mediated hole closure
title_full Nuclear envelope assembly relies on CHMP-7 in the absence of BAF-LEM-mediated hole closure
title_fullStr Nuclear envelope assembly relies on CHMP-7 in the absence of BAF-LEM-mediated hole closure
title_full_unstemmed Nuclear envelope assembly relies on CHMP-7 in the absence of BAF-LEM-mediated hole closure
title_short Nuclear envelope assembly relies on CHMP-7 in the absence of BAF-LEM-mediated hole closure
title_sort nuclear envelope assembly relies on chmp-7 in the absence of baf-lem-mediated hole closure
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10350047/
https://www.ncbi.nlm.nih.gov/pubmed/37461528
http://dx.doi.org/10.1101/2023.07.06.547980
work_keys_str_mv AT bargersarahr nuclearenvelopeassemblyreliesonchmp7intheabsenceofbaflemmediatedholeclosure
AT penfieldlauren nuclearenvelopeassemblyreliesonchmp7intheabsenceofbaflemmediatedholeclosure
AT bahmanyarshirin nuclearenvelopeassemblyreliesonchmp7intheabsenceofbaflemmediatedholeclosure