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Fiberoptic hemodynamic spectroscopy reveals abnormal cerebrovascular reactivity in a freely moving mouse model of Alzheimer’s disease

Many Alzheimer’s disease (AD) patients suffer from altered cerebral blood flow and damaged cerebral vasculature. Cerebrovascular dysfunction could play an important role in this disease. However, the mechanism underlying a vascular contribution in AD is still unclear. Cerebrovascular reactivity (CVR...

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Autores principales: Gareau, Daniel S., RochaKim, Nicholas, Choudhury, Arnab, Bamkole, Michael, Snuderl, Matija, Zou, Julia, Yaroslavsky, Anna, Jacques, Steven L., Strickland, Sidney, Krueger, James G., Ahn, Hyung Jin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10350529/
https://www.ncbi.nlm.nih.gov/pubmed/37465366
http://dx.doi.org/10.3389/fnmol.2023.1163447
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author Gareau, Daniel S.
RochaKim, Nicholas
Choudhury, Arnab
Bamkole, Michael
Snuderl, Matija
Zou, Julia
Yaroslavsky, Anna
Jacques, Steven L.
Strickland, Sidney
Krueger, James G.
Ahn, Hyung Jin
author_facet Gareau, Daniel S.
RochaKim, Nicholas
Choudhury, Arnab
Bamkole, Michael
Snuderl, Matija
Zou, Julia
Yaroslavsky, Anna
Jacques, Steven L.
Strickland, Sidney
Krueger, James G.
Ahn, Hyung Jin
author_sort Gareau, Daniel S.
collection PubMed
description Many Alzheimer’s disease (AD) patients suffer from altered cerebral blood flow and damaged cerebral vasculature. Cerebrovascular dysfunction could play an important role in this disease. However, the mechanism underlying a vascular contribution in AD is still unclear. Cerebrovascular reactivity (CVR) is a critical mechanism that maintains cerebral blood flow and brain homeostasis. Most current methods to analyze CVR require anesthesia which is known to hamper the investigation of molecular mechanisms underlying CVR. We therefore combined spectroscopy, spectral analysis software, and an implantable device to measure cerebral blood volume fraction (CBVF) and oxygen saturation (S(O2)) in unanesthetized, freely-moving mice. Then, we analyzed basal CBVF and S(O2,) and CVR of 5-month-old C57BL/6 mice during hypercapnia as well as during basic behavior such as grooming, walking and running. Moreover, we analyzed the CVR of freely-moving AD mice and their wildtype (WT) littermates during hypercapnia and could find impaired CVR in AD mice compared to WT littermates. Our results suggest that this optomechanical approach to reproducibly getting light into the brain enabled us to successfully measure CVR in unanesthetized freely-moving mice and to find impaired CVR in a mouse model of AD.
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spelling pubmed-103505292023-07-18 Fiberoptic hemodynamic spectroscopy reveals abnormal cerebrovascular reactivity in a freely moving mouse model of Alzheimer’s disease Gareau, Daniel S. RochaKim, Nicholas Choudhury, Arnab Bamkole, Michael Snuderl, Matija Zou, Julia Yaroslavsky, Anna Jacques, Steven L. Strickland, Sidney Krueger, James G. Ahn, Hyung Jin Front Mol Neurosci Molecular Neuroscience Many Alzheimer’s disease (AD) patients suffer from altered cerebral blood flow and damaged cerebral vasculature. Cerebrovascular dysfunction could play an important role in this disease. However, the mechanism underlying a vascular contribution in AD is still unclear. Cerebrovascular reactivity (CVR) is a critical mechanism that maintains cerebral blood flow and brain homeostasis. Most current methods to analyze CVR require anesthesia which is known to hamper the investigation of molecular mechanisms underlying CVR. We therefore combined spectroscopy, spectral analysis software, and an implantable device to measure cerebral blood volume fraction (CBVF) and oxygen saturation (S(O2)) in unanesthetized, freely-moving mice. Then, we analyzed basal CBVF and S(O2,) and CVR of 5-month-old C57BL/6 mice during hypercapnia as well as during basic behavior such as grooming, walking and running. Moreover, we analyzed the CVR of freely-moving AD mice and their wildtype (WT) littermates during hypercapnia and could find impaired CVR in AD mice compared to WT littermates. Our results suggest that this optomechanical approach to reproducibly getting light into the brain enabled us to successfully measure CVR in unanesthetized freely-moving mice and to find impaired CVR in a mouse model of AD. Frontiers Media S.A. 2023-07-03 /pmc/articles/PMC10350529/ /pubmed/37465366 http://dx.doi.org/10.3389/fnmol.2023.1163447 Text en Copyright © 2023 Gareau, RochaKim, Choudhury, Bamkole, Snuderl, Zou, Yaroslavsky, Jacques, Strickland, Krueger and Ahn. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Molecular Neuroscience
Gareau, Daniel S.
RochaKim, Nicholas
Choudhury, Arnab
Bamkole, Michael
Snuderl, Matija
Zou, Julia
Yaroslavsky, Anna
Jacques, Steven L.
Strickland, Sidney
Krueger, James G.
Ahn, Hyung Jin
Fiberoptic hemodynamic spectroscopy reveals abnormal cerebrovascular reactivity in a freely moving mouse model of Alzheimer’s disease
title Fiberoptic hemodynamic spectroscopy reveals abnormal cerebrovascular reactivity in a freely moving mouse model of Alzheimer’s disease
title_full Fiberoptic hemodynamic spectroscopy reveals abnormal cerebrovascular reactivity in a freely moving mouse model of Alzheimer’s disease
title_fullStr Fiberoptic hemodynamic spectroscopy reveals abnormal cerebrovascular reactivity in a freely moving mouse model of Alzheimer’s disease
title_full_unstemmed Fiberoptic hemodynamic spectroscopy reveals abnormal cerebrovascular reactivity in a freely moving mouse model of Alzheimer’s disease
title_short Fiberoptic hemodynamic spectroscopy reveals abnormal cerebrovascular reactivity in a freely moving mouse model of Alzheimer’s disease
title_sort fiberoptic hemodynamic spectroscopy reveals abnormal cerebrovascular reactivity in a freely moving mouse model of alzheimer’s disease
topic Molecular Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10350529/
https://www.ncbi.nlm.nih.gov/pubmed/37465366
http://dx.doi.org/10.3389/fnmol.2023.1163447
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