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Single cell analysis of transcriptome and open chromatin reveals the dynamics of hair follicle stem cell aging

Aging is defined as the functional decline of tissues and organisms, leading to many human conditions, such as cancer, neurodegenerative diseases, and hair loss. Although stem cell exhaustion is widely recognized as a hallmark of aging, our understanding of cell state changes–specifically, the dynam...

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Autores principales: Zhang, Chi, Wang, Dongmei, Dowell, Robin, Yi, Rui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10350644/
https://www.ncbi.nlm.nih.gov/pubmed/37465120
http://dx.doi.org/10.3389/fragi.2023.1192149
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author Zhang, Chi
Wang, Dongmei
Dowell, Robin
Yi, Rui
author_facet Zhang, Chi
Wang, Dongmei
Dowell, Robin
Yi, Rui
author_sort Zhang, Chi
collection PubMed
description Aging is defined as the functional decline of tissues and organisms, leading to many human conditions, such as cancer, neurodegenerative diseases, and hair loss. Although stem cell exhaustion is widely recognized as a hallmark of aging, our understanding of cell state changes–specifically, the dynamics of the transcriptome and open chromatin landscape, and their relationship with aging–remains incomplete. Here we present a longitudinal, single-cell atlas of the transcriptome and open chromatin landscape for epithelia cells of the skin across various hair cycle stages and ages in mice. Our findings reveal fluctuating hair follicle stem cell (HF-SC) states, some of which are associated with the progression of the hair cycle during aging. Conversely, inner bulge niche cells display a more linear progression, seemingly less affected by the hair cycle. Further analysis of the open chromatin landscape, determined by single-cell Assay for Transposase-Accessible Chromatin (ATAC) sequencing, demonstrates that reduced open chromatin regions in HF-SCs are associated with differentiation, whereas gained open chromatin regions in HF-SCs are linked to the transcriptional control of quiescence. These findings enhance our understanding of the transcriptional dynamics in HF-SC aging and lay the molecular groundwork for investigating and potentially reversing the aging process in future experimental studies.
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spelling pubmed-103506442023-07-18 Single cell analysis of transcriptome and open chromatin reveals the dynamics of hair follicle stem cell aging Zhang, Chi Wang, Dongmei Dowell, Robin Yi, Rui Front Aging Aging Aging is defined as the functional decline of tissues and organisms, leading to many human conditions, such as cancer, neurodegenerative diseases, and hair loss. Although stem cell exhaustion is widely recognized as a hallmark of aging, our understanding of cell state changes–specifically, the dynamics of the transcriptome and open chromatin landscape, and their relationship with aging–remains incomplete. Here we present a longitudinal, single-cell atlas of the transcriptome and open chromatin landscape for epithelia cells of the skin across various hair cycle stages and ages in mice. Our findings reveal fluctuating hair follicle stem cell (HF-SC) states, some of which are associated with the progression of the hair cycle during aging. Conversely, inner bulge niche cells display a more linear progression, seemingly less affected by the hair cycle. Further analysis of the open chromatin landscape, determined by single-cell Assay for Transposase-Accessible Chromatin (ATAC) sequencing, demonstrates that reduced open chromatin regions in HF-SCs are associated with differentiation, whereas gained open chromatin regions in HF-SCs are linked to the transcriptional control of quiescence. These findings enhance our understanding of the transcriptional dynamics in HF-SC aging and lay the molecular groundwork for investigating and potentially reversing the aging process in future experimental studies. Frontiers Media S.A. 2023-07-03 /pmc/articles/PMC10350644/ /pubmed/37465120 http://dx.doi.org/10.3389/fragi.2023.1192149 Text en Copyright © 2023 Zhang, Wang, Dowell and Yi. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Aging
Zhang, Chi
Wang, Dongmei
Dowell, Robin
Yi, Rui
Single cell analysis of transcriptome and open chromatin reveals the dynamics of hair follicle stem cell aging
title Single cell analysis of transcriptome and open chromatin reveals the dynamics of hair follicle stem cell aging
title_full Single cell analysis of transcriptome and open chromatin reveals the dynamics of hair follicle stem cell aging
title_fullStr Single cell analysis of transcriptome and open chromatin reveals the dynamics of hair follicle stem cell aging
title_full_unstemmed Single cell analysis of transcriptome and open chromatin reveals the dynamics of hair follicle stem cell aging
title_short Single cell analysis of transcriptome and open chromatin reveals the dynamics of hair follicle stem cell aging
title_sort single cell analysis of transcriptome and open chromatin reveals the dynamics of hair follicle stem cell aging
topic Aging
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10350644/
https://www.ncbi.nlm.nih.gov/pubmed/37465120
http://dx.doi.org/10.3389/fragi.2023.1192149
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