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Electronically Preresonant Stimulated Raman Scattering Microscopy of Weakly Fluorescing Chromophores
[Image: see text] Stimulated Raman Scattering microscopy is an important imaging technique. Its broader application, however, is hampered by its comparatively low sensitivity. Using organic fluorophores, it has recently been demonstrated that, similar to spontaneous Raman microscopy, the sensitivity...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10350953/ https://www.ncbi.nlm.nih.gov/pubmed/37403687 http://dx.doi.org/10.1021/acs.jpcb.3c01407 |
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author | Pruccoli, Andrea Kocademir, Mustafa Winterhalder, Martin J. Zumbusch, Andreas |
author_facet | Pruccoli, Andrea Kocademir, Mustafa Winterhalder, Martin J. Zumbusch, Andreas |
author_sort | Pruccoli, Andrea |
collection | PubMed |
description | [Image: see text] Stimulated Raman Scattering microscopy is an important imaging technique. Its broader application, however, is hampered by its comparatively low sensitivity. Using organic fluorophores, it has recently been demonstrated that, similar to spontaneous Raman microscopy, the sensitivity of stimulated Raman microscopy is increased by orders of magnitudes if electronic preresonances are exploited. In this Article, we show that this approach also works with low quantum yield chromophores. We investigate the relevant photophysics and discuss the background arising from preresonant excitation conditions. Applications of preresonant stimulated Raman scattering microscopy for the imaging of weakly fluorescing labels in fixed and live cells are demonstrated. |
format | Online Article Text |
id | pubmed-10350953 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-103509532023-07-18 Electronically Preresonant Stimulated Raman Scattering Microscopy of Weakly Fluorescing Chromophores Pruccoli, Andrea Kocademir, Mustafa Winterhalder, Martin J. Zumbusch, Andreas J Phys Chem B [Image: see text] Stimulated Raman Scattering microscopy is an important imaging technique. Its broader application, however, is hampered by its comparatively low sensitivity. Using organic fluorophores, it has recently been demonstrated that, similar to spontaneous Raman microscopy, the sensitivity of stimulated Raman microscopy is increased by orders of magnitudes if electronic preresonances are exploited. In this Article, we show that this approach also works with low quantum yield chromophores. We investigate the relevant photophysics and discuss the background arising from preresonant excitation conditions. Applications of preresonant stimulated Raman scattering microscopy for the imaging of weakly fluorescing labels in fixed and live cells are demonstrated. American Chemical Society 2023-07-05 /pmc/articles/PMC10350953/ /pubmed/37403687 http://dx.doi.org/10.1021/acs.jpcb.3c01407 Text en © 2023 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Pruccoli, Andrea Kocademir, Mustafa Winterhalder, Martin J. Zumbusch, Andreas Electronically Preresonant Stimulated Raman Scattering Microscopy of Weakly Fluorescing Chromophores |
title | Electronically Preresonant Stimulated Raman Scattering
Microscopy of Weakly Fluorescing Chromophores |
title_full | Electronically Preresonant Stimulated Raman Scattering
Microscopy of Weakly Fluorescing Chromophores |
title_fullStr | Electronically Preresonant Stimulated Raman Scattering
Microscopy of Weakly Fluorescing Chromophores |
title_full_unstemmed | Electronically Preresonant Stimulated Raman Scattering
Microscopy of Weakly Fluorescing Chromophores |
title_short | Electronically Preresonant Stimulated Raman Scattering
Microscopy of Weakly Fluorescing Chromophores |
title_sort | electronically preresonant stimulated raman scattering
microscopy of weakly fluorescing chromophores |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10350953/ https://www.ncbi.nlm.nih.gov/pubmed/37403687 http://dx.doi.org/10.1021/acs.jpcb.3c01407 |
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