Cargando…
Isolation and identification of active ingredients and biological activity of Dioscorea nipponica Makino
This study reported the isolation and identification of bioactive compounds from Dioscorea nipponica Makino, a plant used in traditional medicine for various ailments. Nine compounds were isolated, including a new compound named as diosniposide E, which was elucidated by analyzing its (1)H-NMR, (13)...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10351190/ https://www.ncbi.nlm.nih.gov/pubmed/37461036 http://dx.doi.org/10.1186/s12906-023-04086-6 |
_version_ | 1785074295365435392 |
---|---|
author | Xia, Guangqing Zhao, Guanshu Pei, Shichun Zheng, Yanping Zang, Hao Li, Li |
author_facet | Xia, Guangqing Zhao, Guanshu Pei, Shichun Zheng, Yanping Zang, Hao Li, Li |
author_sort | Xia, Guangqing |
collection | PubMed |
description | This study reported the isolation and identification of bioactive compounds from Dioscorea nipponica Makino, a plant used in traditional medicine for various ailments. Nine compounds were isolated, including a new compound named as diosniposide E, which was elucidated by analyzing its (1)H-NMR, (13)C-NMR, DEPT, COSY, HMBC and MS data and comparing them with data available in literature. The other eight compounds were identified as known compounds. Theoretical calculations of energy and the generation of a molecular electrostatic potential surface map were employed to assess the antioxidant capacity of nine compounds, the calculation results exhibited that compounds 5 and 6 had strong antioxidant capacities. To further evaluate the antioxidant activities of the investigated compounds, the DPPH and ABTS assays were conducted. The results from the DPPH scavenging activity test revealed that compounds 4–6 exhibited enhanced scavenging activities compared to L-ascorbic acid, while displaying similar efficacy to trolox. Moreover, the ABTS scavenging activities of compounds 4–6 were found to surpass those of L-ascorbic acid and trolox. In terms of α-glucosidase inhibition, compounds 3 and 4 displayed remarkable inhibitory activities that surpassed the effects of acarbose. Additionally, compound 2 exhibited potent anticholinesterase activities, outperforming donepezil. This research provides insights into the potential bioactive compounds present in Dioscorea nipponica Makino and may contribute to its use in traditional medicine. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12906-023-04086-6. |
format | Online Article Text |
id | pubmed-10351190 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-103511902023-07-18 Isolation and identification of active ingredients and biological activity of Dioscorea nipponica Makino Xia, Guangqing Zhao, Guanshu Pei, Shichun Zheng, Yanping Zang, Hao Li, Li BMC Complement Med Ther Research This study reported the isolation and identification of bioactive compounds from Dioscorea nipponica Makino, a plant used in traditional medicine for various ailments. Nine compounds were isolated, including a new compound named as diosniposide E, which was elucidated by analyzing its (1)H-NMR, (13)C-NMR, DEPT, COSY, HMBC and MS data and comparing them with data available in literature. The other eight compounds were identified as known compounds. Theoretical calculations of energy and the generation of a molecular electrostatic potential surface map were employed to assess the antioxidant capacity of nine compounds, the calculation results exhibited that compounds 5 and 6 had strong antioxidant capacities. To further evaluate the antioxidant activities of the investigated compounds, the DPPH and ABTS assays were conducted. The results from the DPPH scavenging activity test revealed that compounds 4–6 exhibited enhanced scavenging activities compared to L-ascorbic acid, while displaying similar efficacy to trolox. Moreover, the ABTS scavenging activities of compounds 4–6 were found to surpass those of L-ascorbic acid and trolox. In terms of α-glucosidase inhibition, compounds 3 and 4 displayed remarkable inhibitory activities that surpassed the effects of acarbose. Additionally, compound 2 exhibited potent anticholinesterase activities, outperforming donepezil. This research provides insights into the potential bioactive compounds present in Dioscorea nipponica Makino and may contribute to its use in traditional medicine. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12906-023-04086-6. BioMed Central 2023-07-17 /pmc/articles/PMC10351190/ /pubmed/37461036 http://dx.doi.org/10.1186/s12906-023-04086-6 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Xia, Guangqing Zhao, Guanshu Pei, Shichun Zheng, Yanping Zang, Hao Li, Li Isolation and identification of active ingredients and biological activity of Dioscorea nipponica Makino |
title | Isolation and identification of active ingredients and biological activity of Dioscorea nipponica Makino |
title_full | Isolation and identification of active ingredients and biological activity of Dioscorea nipponica Makino |
title_fullStr | Isolation and identification of active ingredients and biological activity of Dioscorea nipponica Makino |
title_full_unstemmed | Isolation and identification of active ingredients and biological activity of Dioscorea nipponica Makino |
title_short | Isolation and identification of active ingredients and biological activity of Dioscorea nipponica Makino |
title_sort | isolation and identification of active ingredients and biological activity of dioscorea nipponica makino |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10351190/ https://www.ncbi.nlm.nih.gov/pubmed/37461036 http://dx.doi.org/10.1186/s12906-023-04086-6 |
work_keys_str_mv | AT xiaguangqing isolationandidentificationofactiveingredientsandbiologicalactivityofdioscoreanipponicamakino AT zhaoguanshu isolationandidentificationofactiveingredientsandbiologicalactivityofdioscoreanipponicamakino AT peishichun isolationandidentificationofactiveingredientsandbiologicalactivityofdioscoreanipponicamakino AT zhengyanping isolationandidentificationofactiveingredientsandbiologicalactivityofdioscoreanipponicamakino AT zanghao isolationandidentificationofactiveingredientsandbiologicalactivityofdioscoreanipponicamakino AT lili isolationandidentificationofactiveingredientsandbiologicalactivityofdioscoreanipponicamakino |