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Alteration of Intestinal Microbiota and Hydrogen Sulfide Metabolism in Patients with Hashimoto's Thyroiditis

OBJECTIVE: To analyze the intestinal microbiota and H2S levels in patients with HT. METHODS: Twenty euthyroid HT patients and twenty healthy control individuals were recruited. Fecal samples were collected, and the microbiota was examined using 16S RNA gene sequencing. We also collected serum sample...

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Autores principales: Zhang, Shangqing, Chi, Yan, Jin, Hongfang, Wang, Xiuli, Zhao, Xue, Gao, Ying, Zhang, Junqing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10352533/
https://www.ncbi.nlm.nih.gov/pubmed/37469789
http://dx.doi.org/10.1155/2023/9302189
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author Zhang, Shangqing
Chi, Yan
Jin, Hongfang
Wang, Xiuli
Zhao, Xue
Gao, Ying
Zhang, Junqing
author_facet Zhang, Shangqing
Chi, Yan
Jin, Hongfang
Wang, Xiuli
Zhao, Xue
Gao, Ying
Zhang, Junqing
author_sort Zhang, Shangqing
collection PubMed
description OBJECTIVE: To analyze the intestinal microbiota and H2S levels in patients with HT. METHODS: Twenty euthyroid HT patients and twenty healthy control individuals were recruited. Fecal samples were collected, and the microbiota was examined using 16S RNA gene sequencing. We also collected serum samples to examine the H2S levels. RESULTS: Compared with patients with HT, the ACE and Chao indices were significantly lower in healthy controls (P=0.04, 0.03, respectively). The microbial composition of the HT group differed significantly from that of the healthy group. We observed a significant increase in the proportions of Bacteroides, Fusobacterium, Sutterella, and Veillonella in patients with HT (P < 0.05). Linear discriminant analysis and effect size analysis also revealed that Bacteroides and Ralstonia were enriched in patients with HT. Additionally, patients with HT had significantly lower H2S levels than healthy controls (P < 0.005). The enrichment of H2S anabolism was linked to the alteration of intestinal microbiota in patients with HT. CONCLUSION: We demonstrated that patients with HT have aberrant intestinal microbiome and that H2S anabolism may contribute to HT pathogenesis.
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spelling pubmed-103525332023-07-19 Alteration of Intestinal Microbiota and Hydrogen Sulfide Metabolism in Patients with Hashimoto's Thyroiditis Zhang, Shangqing Chi, Yan Jin, Hongfang Wang, Xiuli Zhao, Xue Gao, Ying Zhang, Junqing J Healthc Eng Research Article OBJECTIVE: To analyze the intestinal microbiota and H2S levels in patients with HT. METHODS: Twenty euthyroid HT patients and twenty healthy control individuals were recruited. Fecal samples were collected, and the microbiota was examined using 16S RNA gene sequencing. We also collected serum samples to examine the H2S levels. RESULTS: Compared with patients with HT, the ACE and Chao indices were significantly lower in healthy controls (P=0.04, 0.03, respectively). The microbial composition of the HT group differed significantly from that of the healthy group. We observed a significant increase in the proportions of Bacteroides, Fusobacterium, Sutterella, and Veillonella in patients with HT (P < 0.05). Linear discriminant analysis and effect size analysis also revealed that Bacteroides and Ralstonia were enriched in patients with HT. Additionally, patients with HT had significantly lower H2S levels than healthy controls (P < 0.005). The enrichment of H2S anabolism was linked to the alteration of intestinal microbiota in patients with HT. CONCLUSION: We demonstrated that patients with HT have aberrant intestinal microbiome and that H2S anabolism may contribute to HT pathogenesis. Hindawi 2023-07-10 /pmc/articles/PMC10352533/ /pubmed/37469789 http://dx.doi.org/10.1155/2023/9302189 Text en Copyright © 2023 Shangqing Zhang et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Zhang, Shangqing
Chi, Yan
Jin, Hongfang
Wang, Xiuli
Zhao, Xue
Gao, Ying
Zhang, Junqing
Alteration of Intestinal Microbiota and Hydrogen Sulfide Metabolism in Patients with Hashimoto's Thyroiditis
title Alteration of Intestinal Microbiota and Hydrogen Sulfide Metabolism in Patients with Hashimoto's Thyroiditis
title_full Alteration of Intestinal Microbiota and Hydrogen Sulfide Metabolism in Patients with Hashimoto's Thyroiditis
title_fullStr Alteration of Intestinal Microbiota and Hydrogen Sulfide Metabolism in Patients with Hashimoto's Thyroiditis
title_full_unstemmed Alteration of Intestinal Microbiota and Hydrogen Sulfide Metabolism in Patients with Hashimoto's Thyroiditis
title_short Alteration of Intestinal Microbiota and Hydrogen Sulfide Metabolism in Patients with Hashimoto's Thyroiditis
title_sort alteration of intestinal microbiota and hydrogen sulfide metabolism in patients with hashimoto's thyroiditis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10352533/
https://www.ncbi.nlm.nih.gov/pubmed/37469789
http://dx.doi.org/10.1155/2023/9302189
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