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Improvement of optimum pH and specific activity of pectate lyase from Bacillus RN.1 using loop replacement

Background: Alkaline pectate lyase plays an important role in papermaking, biological refining and wastewater treatment, but its industrial applications are largely limited owing to its low activity and poor alkali resistance. Methods: The alkaline pectate lyase BspPel from Bacillus RN.1 was heterol...

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Detalles Bibliográficos
Autores principales: Li, Piwu, Wei, Xiaofeng, Wang, Yun, Liu, Hui, Xu, Yanpeng, Zhang, Ziyang, Li, Junlin, Wang, Jianbin, Guo, Chuanzhuang, Sui, Songsen, Wang, Junqing, Wang, Ruiming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10352616/
https://www.ncbi.nlm.nih.gov/pubmed/37469444
http://dx.doi.org/10.3389/fbioe.2023.1242123