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Analysis and Validation of the Prognosis ability of the M7G-Related miRNAs in Lung Adenocarcinoma
BACKGROUND: N7-methylguanosine (m7G) modification plays a crucial role in the development and progression of lung cancers. MicroRNAs (miRNAs) are closely involved in programmed cell death and the mechanism of tumor growth. The m7G-associated miRNAs genes in lung adenocarcinoma (LUAD), and their prog...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
West Asia Organization for Cancer Prevention
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10352730/ https://www.ncbi.nlm.nih.gov/pubmed/37116150 http://dx.doi.org/10.31557/APJCP.2023.24.4.1275 |
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author | Qiu, Xiaowen Chen, Yongting Zhu, Xingzhuang Gong, Zheng Yu, Fengyuan Zhang, Pengfei Song, Yipeng Li, Hongwei |
author_facet | Qiu, Xiaowen Chen, Yongting Zhu, Xingzhuang Gong, Zheng Yu, Fengyuan Zhang, Pengfei Song, Yipeng Li, Hongwei |
author_sort | Qiu, Xiaowen |
collection | PubMed |
description | BACKGROUND: N7-methylguanosine (m7G) modification plays a crucial role in the development and progression of lung cancers. MicroRNAs (miRNAs) are closely involved in programmed cell death and the mechanism of tumor growth. The m7G-associated miRNAs genes in lung adenocarcinoma (LUAD), and their prognosis prediction ability of LUAD, however, had not been investigated. METHODS: The RNA transcriptomes, clinical indices, and immune scores of LUAD patients were searched and downloaded from The Cancer Genome Atlas (TCGA) and the ESTIMATE database. The miRNAs targeting METTL1 and WDR4 were extracted from the TargetScan database. Differentially expressed m7G-related miRNAs were identified and their prediction power of LUAD prognosis was systematically investigated. RESULTS: Among 40 the differentially expressed m7G-related miRNAs in LUAD, five (hsa-miR-31-5p, hsa-miR-5571-3p, hsa-miR-4697-3p, hsa-miR-6858-5p, and hsa-miR-873-3p) demonstrate significant predictive value for prognosis. The risk score constructed by these five miRNAs was an independent prognostic factor (univariate Cox regression results: hazard ratio (HR) = 1.6619, 95% confidential interval (CI) = 1.2103-2.2819, p = 0.0017; multivariate Cox regression results: HR = 1.6004, 95% CI = 1.1633-2.2017, p = 0.0039). The survival curves showed that patients with high-risk scores had a poor prognosis. Calibration curves indicated good predictability in a nomogram constructed combining the miRNA and the clinical indices of age, sex, chemotherapy, radiotherapy, stage, and risk score. GO and KEGG analysis of the overlapping genes showed that the prognostic miRNAs were closely associated with the neuropeptide signaling pathway. Besides, the immune infiltration analysis showed that the expression of the AMPD1 gene was strongly associated with immune cells and immunology functions in LUAD. CONCLUSION: This study identified DE m7G-related miRNAs and demonstrated their prediction ability in the prognosis of LUAD patients. The risk signature based on these miRNAs demonstrates high accuracy in predicting the prognosis of LUAD patients. |
format | Online Article Text |
id | pubmed-10352730 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | West Asia Organization for Cancer Prevention |
record_format | MEDLINE/PubMed |
spelling | pubmed-103527302023-07-19 Analysis and Validation of the Prognosis ability of the M7G-Related miRNAs in Lung Adenocarcinoma Qiu, Xiaowen Chen, Yongting Zhu, Xingzhuang Gong, Zheng Yu, Fengyuan Zhang, Pengfei Song, Yipeng Li, Hongwei Asian Pac J Cancer Prev Research Article BACKGROUND: N7-methylguanosine (m7G) modification plays a crucial role in the development and progression of lung cancers. MicroRNAs (miRNAs) are closely involved in programmed cell death and the mechanism of tumor growth. The m7G-associated miRNAs genes in lung adenocarcinoma (LUAD), and their prognosis prediction ability of LUAD, however, had not been investigated. METHODS: The RNA transcriptomes, clinical indices, and immune scores of LUAD patients were searched and downloaded from The Cancer Genome Atlas (TCGA) and the ESTIMATE database. The miRNAs targeting METTL1 and WDR4 were extracted from the TargetScan database. Differentially expressed m7G-related miRNAs were identified and their prediction power of LUAD prognosis was systematically investigated. RESULTS: Among 40 the differentially expressed m7G-related miRNAs in LUAD, five (hsa-miR-31-5p, hsa-miR-5571-3p, hsa-miR-4697-3p, hsa-miR-6858-5p, and hsa-miR-873-3p) demonstrate significant predictive value for prognosis. The risk score constructed by these five miRNAs was an independent prognostic factor (univariate Cox regression results: hazard ratio (HR) = 1.6619, 95% confidential interval (CI) = 1.2103-2.2819, p = 0.0017; multivariate Cox regression results: HR = 1.6004, 95% CI = 1.1633-2.2017, p = 0.0039). The survival curves showed that patients with high-risk scores had a poor prognosis. Calibration curves indicated good predictability in a nomogram constructed combining the miRNA and the clinical indices of age, sex, chemotherapy, radiotherapy, stage, and risk score. GO and KEGG analysis of the overlapping genes showed that the prognostic miRNAs were closely associated with the neuropeptide signaling pathway. Besides, the immune infiltration analysis showed that the expression of the AMPD1 gene was strongly associated with immune cells and immunology functions in LUAD. CONCLUSION: This study identified DE m7G-related miRNAs and demonstrated their prediction ability in the prognosis of LUAD patients. The risk signature based on these miRNAs demonstrates high accuracy in predicting the prognosis of LUAD patients. West Asia Organization for Cancer Prevention 2023 /pmc/articles/PMC10352730/ /pubmed/37116150 http://dx.doi.org/10.31557/APJCP.2023.24.4.1275 Text en https://creativecommons.org/licenses/by-nc/4.0/This work is licensed under a Creative Commons Attribution-Non Commercial 4.0 International License. (https://creativecommons.org/licenses/by-nc/4.0/) |
spellingShingle | Research Article Qiu, Xiaowen Chen, Yongting Zhu, Xingzhuang Gong, Zheng Yu, Fengyuan Zhang, Pengfei Song, Yipeng Li, Hongwei Analysis and Validation of the Prognosis ability of the M7G-Related miRNAs in Lung Adenocarcinoma |
title | Analysis and Validation of the Prognosis ability of the M7G-Related miRNAs in Lung Adenocarcinoma |
title_full | Analysis and Validation of the Prognosis ability of the M7G-Related miRNAs in Lung Adenocarcinoma |
title_fullStr | Analysis and Validation of the Prognosis ability of the M7G-Related miRNAs in Lung Adenocarcinoma |
title_full_unstemmed | Analysis and Validation of the Prognosis ability of the M7G-Related miRNAs in Lung Adenocarcinoma |
title_short | Analysis and Validation of the Prognosis ability of the M7G-Related miRNAs in Lung Adenocarcinoma |
title_sort | analysis and validation of the prognosis ability of the m7g-related mirnas in lung adenocarcinoma |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10352730/ https://www.ncbi.nlm.nih.gov/pubmed/37116150 http://dx.doi.org/10.31557/APJCP.2023.24.4.1275 |
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