Cargando…

New silicate-substituted hydroxyapatite materials doped with silver ions as potential antifungal agents

BACKGROUND: Hydroxyapatites (HAp) are widely used as medical preparations for e.g., bone replacement or teeth implants. Incorporation of various substrates into HAp structures could enhance its biological properties, like biocompatibility or antimicrobial effects. Silver ions possess high antibacter...

Descripción completa

Detalles Bibliográficos
Autores principales: Piecuch, Agata, Targońska, Sara, Rewak-Sorczyńska, Justyna, Ogórek, Rafał, Wiglusz, Rafal J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10353133/
https://www.ncbi.nlm.nih.gov/pubmed/37464289
http://dx.doi.org/10.1186/s12866-023-02930-w
_version_ 1785074657129398272
author Piecuch, Agata
Targońska, Sara
Rewak-Sorczyńska, Justyna
Ogórek, Rafał
Wiglusz, Rafal J.
author_facet Piecuch, Agata
Targońska, Sara
Rewak-Sorczyńska, Justyna
Ogórek, Rafał
Wiglusz, Rafal J.
author_sort Piecuch, Agata
collection PubMed
description BACKGROUND: Hydroxyapatites (HAp) are widely used as medical preparations for e.g., bone replacement or teeth implants. Incorporation of various substrates into HAp structures could enhance its biological properties, like biocompatibility or antimicrobial effects. Silver ions possess high antibacterial and antifungal activity and its application as HAp dopant might increase its clinical value. RESULTS: New silicate-substituted hydroxyapatites (HAp) doped with silver ions were synthesized via hydrothermal methods. The crystal structure of HAp was investigated by using the X-ray powder diffraction. Antifungal activity of silver ion-doped HAp (with 0.7 mol%, 1 mol% and 2 mol% of dopants) was tested against the yeast-like reference and clinical strains of Candida albicans, C. glabrata, C. tropicalis, Rhodotorula rubra, R. mucilaginosa, Cryptococcus neoformans and C. gattii. Spectrophotometric method was used to evaluate antifungal effect of HAp in SD medium. It was shown that already the lowest dopant (0.7 mol% of Ag(+) ions) significantly reduced fungal growth at the concentration of 100 µg/mL. Increase in the dopant content and the concentration of HAp did not cause further growth inhibition. Moreover, there were some differences at the tolerance level to Ag(+) ion-doped HAp among tested strains, suggesting strain-specific activity. CONCLUSIONS: Preformed studies confirm antimicrobial potential of hydroxyapatite doped with silver. New Ag(+) ion-HAp material could be, after further studies, considered as medical agent with antifungal properties which lower the risk of a surgical-related infections.
format Online
Article
Text
id pubmed-10353133
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-103531332023-07-19 New silicate-substituted hydroxyapatite materials doped with silver ions as potential antifungal agents Piecuch, Agata Targońska, Sara Rewak-Sorczyńska, Justyna Ogórek, Rafał Wiglusz, Rafal J. BMC Microbiol Research BACKGROUND: Hydroxyapatites (HAp) are widely used as medical preparations for e.g., bone replacement or teeth implants. Incorporation of various substrates into HAp structures could enhance its biological properties, like biocompatibility or antimicrobial effects. Silver ions possess high antibacterial and antifungal activity and its application as HAp dopant might increase its clinical value. RESULTS: New silicate-substituted hydroxyapatites (HAp) doped with silver ions were synthesized via hydrothermal methods. The crystal structure of HAp was investigated by using the X-ray powder diffraction. Antifungal activity of silver ion-doped HAp (with 0.7 mol%, 1 mol% and 2 mol% of dopants) was tested against the yeast-like reference and clinical strains of Candida albicans, C. glabrata, C. tropicalis, Rhodotorula rubra, R. mucilaginosa, Cryptococcus neoformans and C. gattii. Spectrophotometric method was used to evaluate antifungal effect of HAp in SD medium. It was shown that already the lowest dopant (0.7 mol% of Ag(+) ions) significantly reduced fungal growth at the concentration of 100 µg/mL. Increase in the dopant content and the concentration of HAp did not cause further growth inhibition. Moreover, there were some differences at the tolerance level to Ag(+) ion-doped HAp among tested strains, suggesting strain-specific activity. CONCLUSIONS: Preformed studies confirm antimicrobial potential of hydroxyapatite doped with silver. New Ag(+) ion-HAp material could be, after further studies, considered as medical agent with antifungal properties which lower the risk of a surgical-related infections. BioMed Central 2023-07-18 /pmc/articles/PMC10353133/ /pubmed/37464289 http://dx.doi.org/10.1186/s12866-023-02930-w Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Piecuch, Agata
Targońska, Sara
Rewak-Sorczyńska, Justyna
Ogórek, Rafał
Wiglusz, Rafal J.
New silicate-substituted hydroxyapatite materials doped with silver ions as potential antifungal agents
title New silicate-substituted hydroxyapatite materials doped with silver ions as potential antifungal agents
title_full New silicate-substituted hydroxyapatite materials doped with silver ions as potential antifungal agents
title_fullStr New silicate-substituted hydroxyapatite materials doped with silver ions as potential antifungal agents
title_full_unstemmed New silicate-substituted hydroxyapatite materials doped with silver ions as potential antifungal agents
title_short New silicate-substituted hydroxyapatite materials doped with silver ions as potential antifungal agents
title_sort new silicate-substituted hydroxyapatite materials doped with silver ions as potential antifungal agents
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10353133/
https://www.ncbi.nlm.nih.gov/pubmed/37464289
http://dx.doi.org/10.1186/s12866-023-02930-w
work_keys_str_mv AT piecuchagata newsilicatesubstitutedhydroxyapatitematerialsdopedwithsilverionsaspotentialantifungalagents
AT targonskasara newsilicatesubstitutedhydroxyapatitematerialsdopedwithsilverionsaspotentialantifungalagents
AT rewaksorczynskajustyna newsilicatesubstitutedhydroxyapatitematerialsdopedwithsilverionsaspotentialantifungalagents
AT ogorekrafał newsilicatesubstitutedhydroxyapatitematerialsdopedwithsilverionsaspotentialantifungalagents
AT wigluszrafalj newsilicatesubstitutedhydroxyapatitematerialsdopedwithsilverionsaspotentialantifungalagents