Cargando…

Gene expression profile of the developing endosperm in durum wheat provides insight into starch biosynthesis

BACKGROUND: Durum wheat (Triticum turgidum subsp. durum) is widely grown for pasta production, and more recently, is gaining additional interest due to its resilience to warm, dry climates and its use as an experimental model for wheat research. Like in bread wheat, the starch and protein accumulate...

Descripción completa

Detalles Bibliográficos
Autores principales: Chen, Jiawen, Watson-Lazowski, Alexander, Kamble, Nitin Uttam, Vickers, Martin, Seung, David
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10353290/
https://www.ncbi.nlm.nih.gov/pubmed/37460981
http://dx.doi.org/10.1186/s12870-023-04369-7
_version_ 1785074684758327296
author Chen, Jiawen
Watson-Lazowski, Alexander
Kamble, Nitin Uttam
Vickers, Martin
Seung, David
author_facet Chen, Jiawen
Watson-Lazowski, Alexander
Kamble, Nitin Uttam
Vickers, Martin
Seung, David
author_sort Chen, Jiawen
collection PubMed
description BACKGROUND: Durum wheat (Triticum turgidum subsp. durum) is widely grown for pasta production, and more recently, is gaining additional interest due to its resilience to warm, dry climates and its use as an experimental model for wheat research. Like in bread wheat, the starch and protein accumulated in the endosperm during grain development are the primary contributors to the calorific value of durum grains. RESULTS: To enable further research into endosperm development and storage reserve synthesis, we generated a high-quality transcriptomics dataset from developing endosperms of variety Kronos, to complement the extensive mutant resources available for this variety. Endosperms were dissected from grains harvested at eight timepoints during grain development (6 to 30 days post anthesis (dpa)), then RNA sequencing was used to profile the transcriptome at each stage. The largest changes in gene expression profile were observed between the earlier timepoints, prior to 15 dpa. We detected a total of 29,925 genes that were significantly differentially expressed between at least two timepoints, and clustering analysis revealed nine distinct expression patterns. We demonstrate the potential of our dataset to provide new insights into key processes that occur during endosperm development, using starch metabolism as an example. CONCLUSION: We provide a valuable resource for studying endosperm development in this increasingly important crop species. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-023-04369-7.
format Online
Article
Text
id pubmed-10353290
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-103532902023-07-19 Gene expression profile of the developing endosperm in durum wheat provides insight into starch biosynthesis Chen, Jiawen Watson-Lazowski, Alexander Kamble, Nitin Uttam Vickers, Martin Seung, David BMC Plant Biol Research BACKGROUND: Durum wheat (Triticum turgidum subsp. durum) is widely grown for pasta production, and more recently, is gaining additional interest due to its resilience to warm, dry climates and its use as an experimental model for wheat research. Like in bread wheat, the starch and protein accumulated in the endosperm during grain development are the primary contributors to the calorific value of durum grains. RESULTS: To enable further research into endosperm development and storage reserve synthesis, we generated a high-quality transcriptomics dataset from developing endosperms of variety Kronos, to complement the extensive mutant resources available for this variety. Endosperms were dissected from grains harvested at eight timepoints during grain development (6 to 30 days post anthesis (dpa)), then RNA sequencing was used to profile the transcriptome at each stage. The largest changes in gene expression profile were observed between the earlier timepoints, prior to 15 dpa. We detected a total of 29,925 genes that were significantly differentially expressed between at least two timepoints, and clustering analysis revealed nine distinct expression patterns. We demonstrate the potential of our dataset to provide new insights into key processes that occur during endosperm development, using starch metabolism as an example. CONCLUSION: We provide a valuable resource for studying endosperm development in this increasingly important crop species. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-023-04369-7. BioMed Central 2023-07-18 /pmc/articles/PMC10353290/ /pubmed/37460981 http://dx.doi.org/10.1186/s12870-023-04369-7 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Chen, Jiawen
Watson-Lazowski, Alexander
Kamble, Nitin Uttam
Vickers, Martin
Seung, David
Gene expression profile of the developing endosperm in durum wheat provides insight into starch biosynthesis
title Gene expression profile of the developing endosperm in durum wheat provides insight into starch biosynthesis
title_full Gene expression profile of the developing endosperm in durum wheat provides insight into starch biosynthesis
title_fullStr Gene expression profile of the developing endosperm in durum wheat provides insight into starch biosynthesis
title_full_unstemmed Gene expression profile of the developing endosperm in durum wheat provides insight into starch biosynthesis
title_short Gene expression profile of the developing endosperm in durum wheat provides insight into starch biosynthesis
title_sort gene expression profile of the developing endosperm in durum wheat provides insight into starch biosynthesis
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10353290/
https://www.ncbi.nlm.nih.gov/pubmed/37460981
http://dx.doi.org/10.1186/s12870-023-04369-7
work_keys_str_mv AT chenjiawen geneexpressionprofileofthedevelopingendospermindurumwheatprovidesinsightintostarchbiosynthesis
AT watsonlazowskialexander geneexpressionprofileofthedevelopingendospermindurumwheatprovidesinsightintostarchbiosynthesis
AT kamblenitinuttam geneexpressionprofileofthedevelopingendospermindurumwheatprovidesinsightintostarchbiosynthesis
AT vickersmartin geneexpressionprofileofthedevelopingendospermindurumwheatprovidesinsightintostarchbiosynthesis
AT seungdavid geneexpressionprofileofthedevelopingendospermindurumwheatprovidesinsightintostarchbiosynthesis