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Development of a rat model of lymphedema and the implantation of a collagen-based medical device for therapeutic intervention

Secondary lymphedema is a common condition among cancer survivors, and treatment strategies to prevent or treat lymphedema are in high demand. The development of novel strategies to diagnose or treat lymphedema would benefit from a robust experimental animal model of secondary lymphedema. The purpos...

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Autores principales: Nguyen, Dung, Dionyssiou, Dimitrios, Zaitseva, Tatiana S., Zhou, Anna T., Sue, Gloria, Deptula, Peter, Moroz, Maxim A., Tabada, Peter, Rockson, Stanley G., Paukshto, Michael V., Cheng, Ming-Huei, Huang, Ngan F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10353614/
https://www.ncbi.nlm.nih.gov/pubmed/37469481
http://dx.doi.org/10.3389/fcvm.2023.1214116
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author Nguyen, Dung
Dionyssiou, Dimitrios
Zaitseva, Tatiana S.
Zhou, Anna T.
Sue, Gloria
Deptula, Peter
Moroz, Maxim A.
Tabada, Peter
Rockson, Stanley G.
Paukshto, Michael V.
Cheng, Ming-Huei
Huang, Ngan F.
author_facet Nguyen, Dung
Dionyssiou, Dimitrios
Zaitseva, Tatiana S.
Zhou, Anna T.
Sue, Gloria
Deptula, Peter
Moroz, Maxim A.
Tabada, Peter
Rockson, Stanley G.
Paukshto, Michael V.
Cheng, Ming-Huei
Huang, Ngan F.
author_sort Nguyen, Dung
collection PubMed
description Secondary lymphedema is a common condition among cancer survivors, and treatment strategies to prevent or treat lymphedema are in high demand. The development of novel strategies to diagnose or treat lymphedema would benefit from a robust experimental animal model of secondary lymphedema. The purpose of this methods paper is to describe and summarize our experience in developing and characterizing a rat hindlimb model of lymphedema. Here we describe a protocol to induce secondary lymphedema that takes advantage of micro computed tomography imaging for limb volume measurements and visualization of lymph drainage with near infrared imaging. To demonstrate the utility of this preclinical model for studying the therapeutic benefit of novel devices, we apply this animal model to test the efficacy of a biomaterials-based implantable medical device.
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spelling pubmed-103536142023-07-19 Development of a rat model of lymphedema and the implantation of a collagen-based medical device for therapeutic intervention Nguyen, Dung Dionyssiou, Dimitrios Zaitseva, Tatiana S. Zhou, Anna T. Sue, Gloria Deptula, Peter Moroz, Maxim A. Tabada, Peter Rockson, Stanley G. Paukshto, Michael V. Cheng, Ming-Huei Huang, Ngan F. Front Cardiovasc Med Cardiovascular Medicine Secondary lymphedema is a common condition among cancer survivors, and treatment strategies to prevent or treat lymphedema are in high demand. The development of novel strategies to diagnose or treat lymphedema would benefit from a robust experimental animal model of secondary lymphedema. The purpose of this methods paper is to describe and summarize our experience in developing and characterizing a rat hindlimb model of lymphedema. Here we describe a protocol to induce secondary lymphedema that takes advantage of micro computed tomography imaging for limb volume measurements and visualization of lymph drainage with near infrared imaging. To demonstrate the utility of this preclinical model for studying the therapeutic benefit of novel devices, we apply this animal model to test the efficacy of a biomaterials-based implantable medical device. Frontiers Media S.A. 2023-07-04 /pmc/articles/PMC10353614/ /pubmed/37469481 http://dx.doi.org/10.3389/fcvm.2023.1214116 Text en © 2023 Nguyen, Dionysiou, Zaitseva, Zhou, Sue, Deptula, Moroz, Tabada, Rockson, Paukshto, Cheng and Huang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) (https://creativecommons.org/licenses/by/4.0/) . The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cardiovascular Medicine
Nguyen, Dung
Dionyssiou, Dimitrios
Zaitseva, Tatiana S.
Zhou, Anna T.
Sue, Gloria
Deptula, Peter
Moroz, Maxim A.
Tabada, Peter
Rockson, Stanley G.
Paukshto, Michael V.
Cheng, Ming-Huei
Huang, Ngan F.
Development of a rat model of lymphedema and the implantation of a collagen-based medical device for therapeutic intervention
title Development of a rat model of lymphedema and the implantation of a collagen-based medical device for therapeutic intervention
title_full Development of a rat model of lymphedema and the implantation of a collagen-based medical device for therapeutic intervention
title_fullStr Development of a rat model of lymphedema and the implantation of a collagen-based medical device for therapeutic intervention
title_full_unstemmed Development of a rat model of lymphedema and the implantation of a collagen-based medical device for therapeutic intervention
title_short Development of a rat model of lymphedema and the implantation of a collagen-based medical device for therapeutic intervention
title_sort development of a rat model of lymphedema and the implantation of a collagen-based medical device for therapeutic intervention
topic Cardiovascular Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10353614/
https://www.ncbi.nlm.nih.gov/pubmed/37469481
http://dx.doi.org/10.3389/fcvm.2023.1214116
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