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Corroborating evidence for aberrant expression of histone deacetylase 8 in endometriosis

PURPOSE: The aim of this study was to evaluate the dynamic change in staining of Class I HDACs and Hdac6 in lesions harvested serially from different time points in mice with induced endometriosis. In addition, the effect of Hdac8 activation as well as Hdac8 and Hdac6 inhibition on lesional progress...

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Autores principales: Zheng, Hanxi, Liu, Xishi, Guo, Sun‐Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10354415/
https://www.ncbi.nlm.nih.gov/pubmed/37476367
http://dx.doi.org/10.1002/rmb2.12527
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author Zheng, Hanxi
Liu, Xishi
Guo, Sun‐Wei
author_facet Zheng, Hanxi
Liu, Xishi
Guo, Sun‐Wei
author_sort Zheng, Hanxi
collection PubMed
description PURPOSE: The aim of this study was to evaluate the dynamic change in staining of Class I HDACs and Hdac6 in lesions harvested serially from different time points in mice with induced endometriosis. In addition, the effect of Hdac8 activation as well as Hdac8 and Hdac6 inhibition on lesional progression and fibrogenesis was evaluated. METHODS: Immunohistochemistry analysis of Class I HDACs and Hdac6 in serially harvested lesion samples in mouse. Hdac8 activation, as well as Hdac6/8 inhibition, was evaluated in mice with induced endometriosis. RESULTS: We found a progressive increase in lesional staining of Hdac1, Hdac8, and Hdac6 and gradual decrease in Hdac2 staining and consistently reduced staining of Hdac3 during the course of lesional progression. The stromal Hdac8 staining correlated most prominently with all markers of lesional fibrosis. Hdac8 activation significantly accelerated the progression and fibrogenesis of endometriotic lesions. In contrast, specific inhibition of Hdac8 or Hdac6, especially of Hdac8, significantly hindered lesional progression and fibrogenesis. CONCLUSIONS: Hdac8 is progressively and aberrantly overexpressed as endometriotic lesions progress. This, along with the documented HDAC1 upregulation in endometriosis and the overwhelming evidence for the therapeutic potentials of HDACIs, calls for further and in‐depth investigation of epigenetic aberrations of endometriosis in general and of HDACs in particular.
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spelling pubmed-103544152023-07-20 Corroborating evidence for aberrant expression of histone deacetylase 8 in endometriosis Zheng, Hanxi Liu, Xishi Guo, Sun‐Wei Reprod Med Biol Original Articles PURPOSE: The aim of this study was to evaluate the dynamic change in staining of Class I HDACs and Hdac6 in lesions harvested serially from different time points in mice with induced endometriosis. In addition, the effect of Hdac8 activation as well as Hdac8 and Hdac6 inhibition on lesional progression and fibrogenesis was evaluated. METHODS: Immunohistochemistry analysis of Class I HDACs and Hdac6 in serially harvested lesion samples in mouse. Hdac8 activation, as well as Hdac6/8 inhibition, was evaluated in mice with induced endometriosis. RESULTS: We found a progressive increase in lesional staining of Hdac1, Hdac8, and Hdac6 and gradual decrease in Hdac2 staining and consistently reduced staining of Hdac3 during the course of lesional progression. The stromal Hdac8 staining correlated most prominently with all markers of lesional fibrosis. Hdac8 activation significantly accelerated the progression and fibrogenesis of endometriotic lesions. In contrast, specific inhibition of Hdac8 or Hdac6, especially of Hdac8, significantly hindered lesional progression and fibrogenesis. CONCLUSIONS: Hdac8 is progressively and aberrantly overexpressed as endometriotic lesions progress. This, along with the documented HDAC1 upregulation in endometriosis and the overwhelming evidence for the therapeutic potentials of HDACIs, calls for further and in‐depth investigation of epigenetic aberrations of endometriosis in general and of HDACs in particular. John Wiley and Sons Inc. 2023-07-18 /pmc/articles/PMC10354415/ /pubmed/37476367 http://dx.doi.org/10.1002/rmb2.12527 Text en © 2023 The Authors. Reproductive Medicine and Biology published by John Wiley & Sons Australia, Ltd on behalf of Japan Society for Reproductive Medicine. https://creativecommons.org/licenses/by-nc/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Original Articles
Zheng, Hanxi
Liu, Xishi
Guo, Sun‐Wei
Corroborating evidence for aberrant expression of histone deacetylase 8 in endometriosis
title Corroborating evidence for aberrant expression of histone deacetylase 8 in endometriosis
title_full Corroborating evidence for aberrant expression of histone deacetylase 8 in endometriosis
title_fullStr Corroborating evidence for aberrant expression of histone deacetylase 8 in endometriosis
title_full_unstemmed Corroborating evidence for aberrant expression of histone deacetylase 8 in endometriosis
title_short Corroborating evidence for aberrant expression of histone deacetylase 8 in endometriosis
title_sort corroborating evidence for aberrant expression of histone deacetylase 8 in endometriosis
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10354415/
https://www.ncbi.nlm.nih.gov/pubmed/37476367
http://dx.doi.org/10.1002/rmb2.12527
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