Cargando…
Biomimetic in situ tracheal microvascularization for segmental tracheal reconstruction in one‐step
Formation of functional and perfusable vascular network is critical to ensure the long‐term survival and functionality of the engineered tissue tracheae after transplantation. However, the greatest challenge in tracheal‐replacement therapy is the promotion of tissue regeneration by rapid graft vascu...
Autores principales: | , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley & Sons, Inc.
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10354772/ https://www.ncbi.nlm.nih.gov/pubmed/37476057 http://dx.doi.org/10.1002/btm2.10534 |
_version_ | 1785074993307058176 |
---|---|
author | Sun, Fei Shen, Zhiming Zhang, Boyou Lu, Yi Shan, Yibo Wu, Qiang Yuan, Lei Zhu, Jianwei Pan, Shu Wang, Zhihao Wu, Cong Zhang, Guozhong Yang, Wenlong Xu, Xiangyu Shi, Hongcan |
author_facet | Sun, Fei Shen, Zhiming Zhang, Boyou Lu, Yi Shan, Yibo Wu, Qiang Yuan, Lei Zhu, Jianwei Pan, Shu Wang, Zhihao Wu, Cong Zhang, Guozhong Yang, Wenlong Xu, Xiangyu Shi, Hongcan |
author_sort | Sun, Fei |
collection | PubMed |
description | Formation of functional and perfusable vascular network is critical to ensure the long‐term survival and functionality of the engineered tissue tracheae after transplantation. However, the greatest challenge in tracheal‐replacement therapy is the promotion of tissue regeneration by rapid graft vascularization. Traditional prevascularization methods for tracheal grafts typically utilize omentum or muscle flap wrapping, which requires a second operation; vascularized segment tracheal orthotopic transplantation in one step remains difficult. This study proposes a method to construct a tissue‐engineered tracheal graft, which directly forms the microvascular network after orthotopic transplantation in vivo. The focus of this study was the preparation of a hybrid tracheal graft that is non‐immunogenic, has good biomechanical properties, supports cell proliferation, and quickly vascularizes. The results showed that vacuum‐assisted decellularized trachea‐polycaprolactone hybrid scaffold could match most of the above requirements as closely as possible. Furthermore, endothelial progenitor cells (EPCs) were extracted and used as vascularized seed cells and seeded on the surfaces of hybrid grafts before and during the tracheal orthotopic transplantation. The results showed that the microvascularized tracheal grafts formed maintained the survival of the recipient, showing a satisfactory therapeutic outcome. This is the first study to utilize EPCs for microvascular construction of long‐segment trachea in one‐step; the approach represents a promising method for microvascular tracheal reconstruction. |
format | Online Article Text |
id | pubmed-10354772 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | John Wiley & Sons, Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-103547722023-07-20 Biomimetic in situ tracheal microvascularization for segmental tracheal reconstruction in one‐step Sun, Fei Shen, Zhiming Zhang, Boyou Lu, Yi Shan, Yibo Wu, Qiang Yuan, Lei Zhu, Jianwei Pan, Shu Wang, Zhihao Wu, Cong Zhang, Guozhong Yang, Wenlong Xu, Xiangyu Shi, Hongcan Bioeng Transl Med Research Articles Formation of functional and perfusable vascular network is critical to ensure the long‐term survival and functionality of the engineered tissue tracheae after transplantation. However, the greatest challenge in tracheal‐replacement therapy is the promotion of tissue regeneration by rapid graft vascularization. Traditional prevascularization methods for tracheal grafts typically utilize omentum or muscle flap wrapping, which requires a second operation; vascularized segment tracheal orthotopic transplantation in one step remains difficult. This study proposes a method to construct a tissue‐engineered tracheal graft, which directly forms the microvascular network after orthotopic transplantation in vivo. The focus of this study was the preparation of a hybrid tracheal graft that is non‐immunogenic, has good biomechanical properties, supports cell proliferation, and quickly vascularizes. The results showed that vacuum‐assisted decellularized trachea‐polycaprolactone hybrid scaffold could match most of the above requirements as closely as possible. Furthermore, endothelial progenitor cells (EPCs) were extracted and used as vascularized seed cells and seeded on the surfaces of hybrid grafts before and during the tracheal orthotopic transplantation. The results showed that the microvascularized tracheal grafts formed maintained the survival of the recipient, showing a satisfactory therapeutic outcome. This is the first study to utilize EPCs for microvascular construction of long‐segment trachea in one‐step; the approach represents a promising method for microvascular tracheal reconstruction. John Wiley & Sons, Inc. 2023-05-03 /pmc/articles/PMC10354772/ /pubmed/37476057 http://dx.doi.org/10.1002/btm2.10534 Text en © 2023 The Authors. Bioengineering & Translational Medicine published by Wiley Periodicals LLC on behalf of American Institute of Chemical Engineers. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Sun, Fei Shen, Zhiming Zhang, Boyou Lu, Yi Shan, Yibo Wu, Qiang Yuan, Lei Zhu, Jianwei Pan, Shu Wang, Zhihao Wu, Cong Zhang, Guozhong Yang, Wenlong Xu, Xiangyu Shi, Hongcan Biomimetic in situ tracheal microvascularization for segmental tracheal reconstruction in one‐step |
title | Biomimetic in situ tracheal microvascularization for segmental tracheal reconstruction in one‐step |
title_full | Biomimetic in situ tracheal microvascularization for segmental tracheal reconstruction in one‐step |
title_fullStr | Biomimetic in situ tracheal microvascularization for segmental tracheal reconstruction in one‐step |
title_full_unstemmed | Biomimetic in situ tracheal microvascularization for segmental tracheal reconstruction in one‐step |
title_short | Biomimetic in situ tracheal microvascularization for segmental tracheal reconstruction in one‐step |
title_sort | biomimetic in situ tracheal microvascularization for segmental tracheal reconstruction in one‐step |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10354772/ https://www.ncbi.nlm.nih.gov/pubmed/37476057 http://dx.doi.org/10.1002/btm2.10534 |
work_keys_str_mv | AT sunfei biomimeticinsitutrachealmicrovascularizationforsegmentaltrachealreconstructioninonestep AT shenzhiming biomimeticinsitutrachealmicrovascularizationforsegmentaltrachealreconstructioninonestep AT zhangboyou biomimeticinsitutrachealmicrovascularizationforsegmentaltrachealreconstructioninonestep AT luyi biomimeticinsitutrachealmicrovascularizationforsegmentaltrachealreconstructioninonestep AT shanyibo biomimeticinsitutrachealmicrovascularizationforsegmentaltrachealreconstructioninonestep AT wuqiang biomimeticinsitutrachealmicrovascularizationforsegmentaltrachealreconstructioninonestep AT yuanlei biomimeticinsitutrachealmicrovascularizationforsegmentaltrachealreconstructioninonestep AT zhujianwei biomimeticinsitutrachealmicrovascularizationforsegmentaltrachealreconstructioninonestep AT panshu biomimeticinsitutrachealmicrovascularizationforsegmentaltrachealreconstructioninonestep AT wangzhihao biomimeticinsitutrachealmicrovascularizationforsegmentaltrachealreconstructioninonestep AT wucong biomimeticinsitutrachealmicrovascularizationforsegmentaltrachealreconstructioninonestep AT zhangguozhong biomimeticinsitutrachealmicrovascularizationforsegmentaltrachealreconstructioninonestep AT yangwenlong biomimeticinsitutrachealmicrovascularizationforsegmentaltrachealreconstructioninonestep AT xuxiangyu biomimeticinsitutrachealmicrovascularizationforsegmentaltrachealreconstructioninonestep AT shihongcan biomimeticinsitutrachealmicrovascularizationforsegmentaltrachealreconstructioninonestep |