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Partial liver irradiation in rats induces the hypertrophy of nonirradiated liver lobes through hepatocyte proliferation(†)

Irradiation of the liver induces a regenerative response in the nonirradiated part of the liver. It is unclear whether this leads to actual liver enlargement. The aim of this study was to evaluate the weight of compensatory hypertrophy that occurs in nonirradiated livers and to clarify the mechanism...

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Autores principales: Adachi, Takuya, Yoshida, Yukari, Shibuya, Kei, Sato, Ken, Yamazaki, Yuichi, Kakizaki, Satoru, Uraoka, Toshio, Takahashi, Akihisa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10354842/
https://www.ncbi.nlm.nih.gov/pubmed/37427542
http://dx.doi.org/10.1093/jrr/rrad051
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author Adachi, Takuya
Yoshida, Yukari
Shibuya, Kei
Sato, Ken
Yamazaki, Yuichi
Kakizaki, Satoru
Uraoka, Toshio
Takahashi, Akihisa
author_facet Adachi, Takuya
Yoshida, Yukari
Shibuya, Kei
Sato, Ken
Yamazaki, Yuichi
Kakizaki, Satoru
Uraoka, Toshio
Takahashi, Akihisa
author_sort Adachi, Takuya
collection PubMed
description Irradiation of the liver induces a regenerative response in the nonirradiated part of the liver. It is unclear whether this leads to actual liver enlargement. The aim of this study was to evaluate the weight of compensatory hypertrophy that occurs in nonirradiated livers and to clarify the mechanism of hypertrophy from the viewpoint of hepatocyte proliferation. The anterior liver lobes (anterior lobes) were irradiated with 60 Gy of X-rays (X60 Gy) under opening laparotomy. Body weights and liver lobe weights were measured before and at 1, 4, 8 and 12 weeks after irradiation, and serum and liver tissue samples were analyzed at each time point. The anterior lobes atrophied progressively, whereas the posterior liver lobes (posterior lobes) hypertrophied in the X-ray irradiated (X-irradiated) group. Although temporary liver damage was observed after irradiation, liver function did not decrease at any time point. Hepatocyte degeneration and loss were observed in the anterior lobes of the X-irradiated group, and significant fibrosis developed 8 weeks postirradiation. Following irradiation, the proportion of Ki-67-positive cells in the anterior lobes decreased markedly in the early postirradiation period, whereas the proportion of positive cells in the posterior lobes increased, peaking at 4 weeks postirradiation (P < 0.05). Increased tumor necrosis factor-α expression was observed only in the anterior liver lobes of the X-irradiated group at 1 and 4 weeks postirradiation. Partial liver irradiation with X60 Gy induced compensatory hypertrophy of nonirradiated liver lobes. This study suggests that liver hypertrophy after partial liver irradiation is caused by increased hepatocyte mitosis.
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spelling pubmed-103548422023-07-20 Partial liver irradiation in rats induces the hypertrophy of nonirradiated liver lobes through hepatocyte proliferation(†) Adachi, Takuya Yoshida, Yukari Shibuya, Kei Sato, Ken Yamazaki, Yuichi Kakizaki, Satoru Uraoka, Toshio Takahashi, Akihisa J Radiat Res Regular paper Irradiation of the liver induces a regenerative response in the nonirradiated part of the liver. It is unclear whether this leads to actual liver enlargement. The aim of this study was to evaluate the weight of compensatory hypertrophy that occurs in nonirradiated livers and to clarify the mechanism of hypertrophy from the viewpoint of hepatocyte proliferation. The anterior liver lobes (anterior lobes) were irradiated with 60 Gy of X-rays (X60 Gy) under opening laparotomy. Body weights and liver lobe weights were measured before and at 1, 4, 8 and 12 weeks after irradiation, and serum and liver tissue samples were analyzed at each time point. The anterior lobes atrophied progressively, whereas the posterior liver lobes (posterior lobes) hypertrophied in the X-ray irradiated (X-irradiated) group. Although temporary liver damage was observed after irradiation, liver function did not decrease at any time point. Hepatocyte degeneration and loss were observed in the anterior lobes of the X-irradiated group, and significant fibrosis developed 8 weeks postirradiation. Following irradiation, the proportion of Ki-67-positive cells in the anterior lobes decreased markedly in the early postirradiation period, whereas the proportion of positive cells in the posterior lobes increased, peaking at 4 weeks postirradiation (P < 0.05). Increased tumor necrosis factor-α expression was observed only in the anterior liver lobes of the X-irradiated group at 1 and 4 weeks postirradiation. Partial liver irradiation with X60 Gy induced compensatory hypertrophy of nonirradiated liver lobes. This study suggests that liver hypertrophy after partial liver irradiation is caused by increased hepatocyte mitosis. Oxford University Press 2023-07-08 /pmc/articles/PMC10354842/ /pubmed/37427542 http://dx.doi.org/10.1093/jrr/rrad051 Text en © The Author(s) 2023. Published by Oxford University Press on behalf of The Japanese Radiation Research Society and Japanese Society for Radiation Oncology. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Regular paper
Adachi, Takuya
Yoshida, Yukari
Shibuya, Kei
Sato, Ken
Yamazaki, Yuichi
Kakizaki, Satoru
Uraoka, Toshio
Takahashi, Akihisa
Partial liver irradiation in rats induces the hypertrophy of nonirradiated liver lobes through hepatocyte proliferation(†)
title Partial liver irradiation in rats induces the hypertrophy of nonirradiated liver lobes through hepatocyte proliferation(†)
title_full Partial liver irradiation in rats induces the hypertrophy of nonirradiated liver lobes through hepatocyte proliferation(†)
title_fullStr Partial liver irradiation in rats induces the hypertrophy of nonirradiated liver lobes through hepatocyte proliferation(†)
title_full_unstemmed Partial liver irradiation in rats induces the hypertrophy of nonirradiated liver lobes through hepatocyte proliferation(†)
title_short Partial liver irradiation in rats induces the hypertrophy of nonirradiated liver lobes through hepatocyte proliferation(†)
title_sort partial liver irradiation in rats induces the hypertrophy of nonirradiated liver lobes through hepatocyte proliferation(†)
topic Regular paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10354842/
https://www.ncbi.nlm.nih.gov/pubmed/37427542
http://dx.doi.org/10.1093/jrr/rrad051
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