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Real-time cell metabolism assessed repeatedly on the same cells via para-hydrogen induced polarization

Signal-enhanced or hyperpolarized nuclear magnetic resonance (NMR) spectroscopy stands out as a unique tool to monitor real-time enzymatic reactions in living cells. The singlet state of para-hydrogen is thereby one source of spin order that can be converted into largely enhanced signals of e.g. met...

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Autores principales: Ding, Yonghong, Stevanato, Gabriele, von Bonin, Frederike, Kube, Dieter, Glöggler, Stefan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10355108/
https://www.ncbi.nlm.nih.gov/pubmed/37476713
http://dx.doi.org/10.1039/d3sc01350b
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author Ding, Yonghong
Stevanato, Gabriele
von Bonin, Frederike
Kube, Dieter
Glöggler, Stefan
author_facet Ding, Yonghong
Stevanato, Gabriele
von Bonin, Frederike
Kube, Dieter
Glöggler, Stefan
author_sort Ding, Yonghong
collection PubMed
description Signal-enhanced or hyperpolarized nuclear magnetic resonance (NMR) spectroscopy stands out as a unique tool to monitor real-time enzymatic reactions in living cells. The singlet state of para-hydrogen is thereby one source of spin order that can be converted into largely enhanced signals of e.g. metabolites. Here, we have investigated a parahydrogen-induced polarization (PHIP) approach as a biological assay for in vitro cellular metabolic characterization. Here, we demonstrate the possibility to perform consecutive measurements yielding metabolic information on the same sample. We observed a strongly reduced pyruvate-to-lactate conversion rate (flux) of a Hodgkin's lymphoma cancer cell line L1236 treated with FK866, an inhibitor of nicotinamide phosphoribosyltransferase (NAMPT) affecting the amount of NAD(+) and thus NADH in cells. In the consecutive measurement the flux was recovered by NADH to the same amount as in the single-measurement-per-sample and provides a promising new analytical tool for continuous real-time studies combinable with bioreactors and lab-on-a-chip devices in the future.
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spelling pubmed-103551082023-07-20 Real-time cell metabolism assessed repeatedly on the same cells via para-hydrogen induced polarization Ding, Yonghong Stevanato, Gabriele von Bonin, Frederike Kube, Dieter Glöggler, Stefan Chem Sci Chemistry Signal-enhanced or hyperpolarized nuclear magnetic resonance (NMR) spectroscopy stands out as a unique tool to monitor real-time enzymatic reactions in living cells. The singlet state of para-hydrogen is thereby one source of spin order that can be converted into largely enhanced signals of e.g. metabolites. Here, we have investigated a parahydrogen-induced polarization (PHIP) approach as a biological assay for in vitro cellular metabolic characterization. Here, we demonstrate the possibility to perform consecutive measurements yielding metabolic information on the same sample. We observed a strongly reduced pyruvate-to-lactate conversion rate (flux) of a Hodgkin's lymphoma cancer cell line L1236 treated with FK866, an inhibitor of nicotinamide phosphoribosyltransferase (NAMPT) affecting the amount of NAD(+) and thus NADH in cells. In the consecutive measurement the flux was recovered by NADH to the same amount as in the single-measurement-per-sample and provides a promising new analytical tool for continuous real-time studies combinable with bioreactors and lab-on-a-chip devices in the future. The Royal Society of Chemistry 2023-06-20 /pmc/articles/PMC10355108/ /pubmed/37476713 http://dx.doi.org/10.1039/d3sc01350b Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/
spellingShingle Chemistry
Ding, Yonghong
Stevanato, Gabriele
von Bonin, Frederike
Kube, Dieter
Glöggler, Stefan
Real-time cell metabolism assessed repeatedly on the same cells via para-hydrogen induced polarization
title Real-time cell metabolism assessed repeatedly on the same cells via para-hydrogen induced polarization
title_full Real-time cell metabolism assessed repeatedly on the same cells via para-hydrogen induced polarization
title_fullStr Real-time cell metabolism assessed repeatedly on the same cells via para-hydrogen induced polarization
title_full_unstemmed Real-time cell metabolism assessed repeatedly on the same cells via para-hydrogen induced polarization
title_short Real-time cell metabolism assessed repeatedly on the same cells via para-hydrogen induced polarization
title_sort real-time cell metabolism assessed repeatedly on the same cells via para-hydrogen induced polarization
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10355108/
https://www.ncbi.nlm.nih.gov/pubmed/37476713
http://dx.doi.org/10.1039/d3sc01350b
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