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Real-time cell metabolism assessed repeatedly on the same cells via para-hydrogen induced polarization
Signal-enhanced or hyperpolarized nuclear magnetic resonance (NMR) spectroscopy stands out as a unique tool to monitor real-time enzymatic reactions in living cells. The singlet state of para-hydrogen is thereby one source of spin order that can be converted into largely enhanced signals of e.g. met...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society of Chemistry
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10355108/ https://www.ncbi.nlm.nih.gov/pubmed/37476713 http://dx.doi.org/10.1039/d3sc01350b |
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author | Ding, Yonghong Stevanato, Gabriele von Bonin, Frederike Kube, Dieter Glöggler, Stefan |
author_facet | Ding, Yonghong Stevanato, Gabriele von Bonin, Frederike Kube, Dieter Glöggler, Stefan |
author_sort | Ding, Yonghong |
collection | PubMed |
description | Signal-enhanced or hyperpolarized nuclear magnetic resonance (NMR) spectroscopy stands out as a unique tool to monitor real-time enzymatic reactions in living cells. The singlet state of para-hydrogen is thereby one source of spin order that can be converted into largely enhanced signals of e.g. metabolites. Here, we have investigated a parahydrogen-induced polarization (PHIP) approach as a biological assay for in vitro cellular metabolic characterization. Here, we demonstrate the possibility to perform consecutive measurements yielding metabolic information on the same sample. We observed a strongly reduced pyruvate-to-lactate conversion rate (flux) of a Hodgkin's lymphoma cancer cell line L1236 treated with FK866, an inhibitor of nicotinamide phosphoribosyltransferase (NAMPT) affecting the amount of NAD(+) and thus NADH in cells. In the consecutive measurement the flux was recovered by NADH to the same amount as in the single-measurement-per-sample and provides a promising new analytical tool for continuous real-time studies combinable with bioreactors and lab-on-a-chip devices in the future. |
format | Online Article Text |
id | pubmed-10355108 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | The Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-103551082023-07-20 Real-time cell metabolism assessed repeatedly on the same cells via para-hydrogen induced polarization Ding, Yonghong Stevanato, Gabriele von Bonin, Frederike Kube, Dieter Glöggler, Stefan Chem Sci Chemistry Signal-enhanced or hyperpolarized nuclear magnetic resonance (NMR) spectroscopy stands out as a unique tool to monitor real-time enzymatic reactions in living cells. The singlet state of para-hydrogen is thereby one source of spin order that can be converted into largely enhanced signals of e.g. metabolites. Here, we have investigated a parahydrogen-induced polarization (PHIP) approach as a biological assay for in vitro cellular metabolic characterization. Here, we demonstrate the possibility to perform consecutive measurements yielding metabolic information on the same sample. We observed a strongly reduced pyruvate-to-lactate conversion rate (flux) of a Hodgkin's lymphoma cancer cell line L1236 treated with FK866, an inhibitor of nicotinamide phosphoribosyltransferase (NAMPT) affecting the amount of NAD(+) and thus NADH in cells. In the consecutive measurement the flux was recovered by NADH to the same amount as in the single-measurement-per-sample and provides a promising new analytical tool for continuous real-time studies combinable with bioreactors and lab-on-a-chip devices in the future. The Royal Society of Chemistry 2023-06-20 /pmc/articles/PMC10355108/ /pubmed/37476713 http://dx.doi.org/10.1039/d3sc01350b Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/ |
spellingShingle | Chemistry Ding, Yonghong Stevanato, Gabriele von Bonin, Frederike Kube, Dieter Glöggler, Stefan Real-time cell metabolism assessed repeatedly on the same cells via para-hydrogen induced polarization |
title | Real-time cell metabolism assessed repeatedly on the same cells via para-hydrogen induced polarization |
title_full | Real-time cell metabolism assessed repeatedly on the same cells via para-hydrogen induced polarization |
title_fullStr | Real-time cell metabolism assessed repeatedly on the same cells via para-hydrogen induced polarization |
title_full_unstemmed | Real-time cell metabolism assessed repeatedly on the same cells via para-hydrogen induced polarization |
title_short | Real-time cell metabolism assessed repeatedly on the same cells via para-hydrogen induced polarization |
title_sort | real-time cell metabolism assessed repeatedly on the same cells via para-hydrogen induced polarization |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10355108/ https://www.ncbi.nlm.nih.gov/pubmed/37476713 http://dx.doi.org/10.1039/d3sc01350b |
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