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miR-21-5p Inhibits the Proliferation, Migration, and Invasion of Glioma by Targeting S100A10

S100A10, a member of the S100 protein family, is upregulated in multiple human malignancies and plays a key role in regulating tumor progression. This study aimed to reveal the underlying mechanism by which S100A10 in regulates the proliferation, migration, and invasion of glioma. The expression and...

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Autores principales: Gao, Peng, Wang, Haopeng, Li, Huaixu, Shu, Lei, Han, Zhenyu, Li, Shiting, Cheng, Hongwei, Dai, Xingliang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10355203/
https://www.ncbi.nlm.nih.gov/pubmed/37476183
http://dx.doi.org/10.7150/jca.84030
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author Gao, Peng
Wang, Haopeng
Li, Huaixu
Shu, Lei
Han, Zhenyu
Li, Shiting
Cheng, Hongwei
Dai, Xingliang
author_facet Gao, Peng
Wang, Haopeng
Li, Huaixu
Shu, Lei
Han, Zhenyu
Li, Shiting
Cheng, Hongwei
Dai, Xingliang
author_sort Gao, Peng
collection PubMed
description S100A10, a member of the S100 protein family, is upregulated in multiple human malignancies and plays a key role in regulating tumor progression. This study aimed to reveal the underlying mechanism by which S100A10 in regulates the proliferation, migration, and invasion of glioma. The expression and clinical information data of S100A10 were downloaded from public databases (TCGA, CGGA, and GEPIA2). S100A10 expression levels in glioma tumor tissues and adjacent nontumor tissues were compared by immunohistochemistry (IHC). The functional roles of S100A10 in glioma were assessed by cell counting kit-8 (CCK-8) cell proliferation assay, wound healing assay, transwell assay, and flow cytometry. miRDB and double luciferase assay were used to predict and identify potential S100A10 mRNA-complementary miRNAs, and the roles of miR-21-5p in glioma cell were examined by targeted knockdown or overexpression miR-21-5p in glioma cell lines. We found that S100A10 was overexpressed in glioma tissues and predicted a worse prognosis. S100A10 knockdown significantly inhibited glioma cell proliferation, invasion, and migration. Furthermore, we demonstrated that miR-21-5p inhibits glioma proliferation, migration, and invasion by targeting S100A10. This study showed S100A10 was a new prognostic predictor among glioma patients and provided new insights into the pathogenesis of gliomas, suggesting that miR-21-5p /S100A10 axis may serve as a valuable therapeutic target for glioma.
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spelling pubmed-103552032023-07-20 miR-21-5p Inhibits the Proliferation, Migration, and Invasion of Glioma by Targeting S100A10 Gao, Peng Wang, Haopeng Li, Huaixu Shu, Lei Han, Zhenyu Li, Shiting Cheng, Hongwei Dai, Xingliang J Cancer Research Paper S100A10, a member of the S100 protein family, is upregulated in multiple human malignancies and plays a key role in regulating tumor progression. This study aimed to reveal the underlying mechanism by which S100A10 in regulates the proliferation, migration, and invasion of glioma. The expression and clinical information data of S100A10 were downloaded from public databases (TCGA, CGGA, and GEPIA2). S100A10 expression levels in glioma tumor tissues and adjacent nontumor tissues were compared by immunohistochemistry (IHC). The functional roles of S100A10 in glioma were assessed by cell counting kit-8 (CCK-8) cell proliferation assay, wound healing assay, transwell assay, and flow cytometry. miRDB and double luciferase assay were used to predict and identify potential S100A10 mRNA-complementary miRNAs, and the roles of miR-21-5p in glioma cell were examined by targeted knockdown or overexpression miR-21-5p in glioma cell lines. We found that S100A10 was overexpressed in glioma tissues and predicted a worse prognosis. S100A10 knockdown significantly inhibited glioma cell proliferation, invasion, and migration. Furthermore, we demonstrated that miR-21-5p inhibits glioma proliferation, migration, and invasion by targeting S100A10. This study showed S100A10 was a new prognostic predictor among glioma patients and provided new insights into the pathogenesis of gliomas, suggesting that miR-21-5p /S100A10 axis may serve as a valuable therapeutic target for glioma. Ivyspring International Publisher 2023-06-19 /pmc/articles/PMC10355203/ /pubmed/37476183 http://dx.doi.org/10.7150/jca.84030 Text en © The author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.
spellingShingle Research Paper
Gao, Peng
Wang, Haopeng
Li, Huaixu
Shu, Lei
Han, Zhenyu
Li, Shiting
Cheng, Hongwei
Dai, Xingliang
miR-21-5p Inhibits the Proliferation, Migration, and Invasion of Glioma by Targeting S100A10
title miR-21-5p Inhibits the Proliferation, Migration, and Invasion of Glioma by Targeting S100A10
title_full miR-21-5p Inhibits the Proliferation, Migration, and Invasion of Glioma by Targeting S100A10
title_fullStr miR-21-5p Inhibits the Proliferation, Migration, and Invasion of Glioma by Targeting S100A10
title_full_unstemmed miR-21-5p Inhibits the Proliferation, Migration, and Invasion of Glioma by Targeting S100A10
title_short miR-21-5p Inhibits the Proliferation, Migration, and Invasion of Glioma by Targeting S100A10
title_sort mir-21-5p inhibits the proliferation, migration, and invasion of glioma by targeting s100a10
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10355203/
https://www.ncbi.nlm.nih.gov/pubmed/37476183
http://dx.doi.org/10.7150/jca.84030
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