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Analysis of antimicrobial efficacy of sodium hypochlorite and ozonated water against biofilm in oval canals

This in vitro study compared the antimicrobial efficacy of 2.5% sodium hypochlorite (NaOCl) and 8 µg/mL ozonated water agitated by passive ultrasonic irrigation (PUI) or PUI combined with EndoActivator (EA) against mature multispecies biofilm. One hundred and five oval-shaped mandibular premolars we...

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Autores principales: Merçon, Ivy Rodrigues, de Campos, Francisco Ubiratan Ferreira, Fontana, Carlos Eduardo, Pelegrine, Rina Andréa, Martin, Alexandre Sigrist De, Bueno, Carlos Eduardo da Silveira
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Fundação Odontológica de Ribeirão Preto 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10355262/
https://www.ncbi.nlm.nih.gov/pubmed/37466523
http://dx.doi.org/10.1590/0103-6440202305318
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author Merçon, Ivy Rodrigues
de Campos, Francisco Ubiratan Ferreira
Fontana, Carlos Eduardo
Pelegrine, Rina Andréa
Martin, Alexandre Sigrist De
Bueno, Carlos Eduardo da Silveira
author_facet Merçon, Ivy Rodrigues
de Campos, Francisco Ubiratan Ferreira
Fontana, Carlos Eduardo
Pelegrine, Rina Andréa
Martin, Alexandre Sigrist De
Bueno, Carlos Eduardo da Silveira
author_sort Merçon, Ivy Rodrigues
collection PubMed
description This in vitro study compared the antimicrobial efficacy of 2.5% sodium hypochlorite (NaOCl) and 8 µg/mL ozonated water agitated by passive ultrasonic irrigation (PUI) or PUI combined with EndoActivator (EA) against mature multispecies biofilm. One hundred and five oval-shaped mandibular premolars were instrumented, sterilized, and inoculated with Enterococcus faecalis, Candida albicans, and Staphylococcus aureus, divided into: control group - saline; O(3) group - ozonated water; O(3) PUI group - ozonated water with PUI agitation; O(3) PUI+EA group - ozonated water with PUI+EA agitation; NaOCl group - NaOCl; NaOCl PUI group - NaOCl with PUI agitation; and NaOCl PUI+EA group - NaOCl with PUI+EA agitation. Microbiological samples were collected before (S1) and after (S2) the disinfection procedures and the data were statistically analyzed using the Kruskal-Wallis test. In the culture method, there was significant disinfection in the O(3) PUI+EA, NaOCl, NaOCl PUI, and NaOCl PUI+EA groups (p˂0.05). The combination of NaOCl with PUI+EA reduced microbial counts to zero (p˂0.05). In the qPCR method, there was a significant reduction in the total count of viable microorganisms in the O(3) PUI, O(3) PUI+EA, NaOCl, NaOCl PUI, and NaOCl PUI+EA groups (p˂0.05). It can be concluded that 2.5% NaOCl with and without agitation, as well as 8 µg/mL ozonated water with its action enhanced by the agitation techniques, were effective in root canal disinfection, and their antimicrobial efficacy is related to the microorganisms present in the biofilm.
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spelling pubmed-103552622023-07-20 Analysis of antimicrobial efficacy of sodium hypochlorite and ozonated water against biofilm in oval canals Merçon, Ivy Rodrigues de Campos, Francisco Ubiratan Ferreira Fontana, Carlos Eduardo Pelegrine, Rina Andréa Martin, Alexandre Sigrist De Bueno, Carlos Eduardo da Silveira Braz Dent J Article This in vitro study compared the antimicrobial efficacy of 2.5% sodium hypochlorite (NaOCl) and 8 µg/mL ozonated water agitated by passive ultrasonic irrigation (PUI) or PUI combined with EndoActivator (EA) against mature multispecies biofilm. One hundred and five oval-shaped mandibular premolars were instrumented, sterilized, and inoculated with Enterococcus faecalis, Candida albicans, and Staphylococcus aureus, divided into: control group - saline; O(3) group - ozonated water; O(3) PUI group - ozonated water with PUI agitation; O(3) PUI+EA group - ozonated water with PUI+EA agitation; NaOCl group - NaOCl; NaOCl PUI group - NaOCl with PUI agitation; and NaOCl PUI+EA group - NaOCl with PUI+EA agitation. Microbiological samples were collected before (S1) and after (S2) the disinfection procedures and the data were statistically analyzed using the Kruskal-Wallis test. In the culture method, there was significant disinfection in the O(3) PUI+EA, NaOCl, NaOCl PUI, and NaOCl PUI+EA groups (p˂0.05). The combination of NaOCl with PUI+EA reduced microbial counts to zero (p˂0.05). In the qPCR method, there was a significant reduction in the total count of viable microorganisms in the O(3) PUI, O(3) PUI+EA, NaOCl, NaOCl PUI, and NaOCl PUI+EA groups (p˂0.05). It can be concluded that 2.5% NaOCl with and without agitation, as well as 8 µg/mL ozonated water with its action enhanced by the agitation techniques, were effective in root canal disinfection, and their antimicrobial efficacy is related to the microorganisms present in the biofilm. Fundação Odontológica de Ribeirão Preto 2023-07-17 /pmc/articles/PMC10355262/ /pubmed/37466523 http://dx.doi.org/10.1590/0103-6440202305318 Text en https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License
spellingShingle Article
Merçon, Ivy Rodrigues
de Campos, Francisco Ubiratan Ferreira
Fontana, Carlos Eduardo
Pelegrine, Rina Andréa
Martin, Alexandre Sigrist De
Bueno, Carlos Eduardo da Silveira
Analysis of antimicrobial efficacy of sodium hypochlorite and ozonated water against biofilm in oval canals
title Analysis of antimicrobial efficacy of sodium hypochlorite and ozonated water against biofilm in oval canals
title_full Analysis of antimicrobial efficacy of sodium hypochlorite and ozonated water against biofilm in oval canals
title_fullStr Analysis of antimicrobial efficacy of sodium hypochlorite and ozonated water against biofilm in oval canals
title_full_unstemmed Analysis of antimicrobial efficacy of sodium hypochlorite and ozonated water against biofilm in oval canals
title_short Analysis of antimicrobial efficacy of sodium hypochlorite and ozonated water against biofilm in oval canals
title_sort analysis of antimicrobial efficacy of sodium hypochlorite and ozonated water against biofilm in oval canals
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10355262/
https://www.ncbi.nlm.nih.gov/pubmed/37466523
http://dx.doi.org/10.1590/0103-6440202305318
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