Cargando…

Analysis of the cervical microbiome in women from the German national cervical cancer screening program

PURPOSE: Cervical cancer (CC) is caused by a persistent high-risk human papillomavirus (hrHPV) infection. The cervico-vaginal microbiome may influence the development of (pre)cancer lesions. Aim of the study was (i) to evaluate the new CC screening program in Germany for the detection of high-grade...

Descripción completa

Detalles Bibliográficos
Autores principales: Condic, Mateja, Neidhöfer, Claudio, Ralser, Damian J., Wetzig, Nina, Thiele, Ralf, Sieber, Martin, Otten, Lucia A., Warwas, Leonie K., Hoerauf, Achim, Mustea, Alexander, Parčina, Marijo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10356625/
https://www.ncbi.nlm.nih.gov/pubmed/36780053
http://dx.doi.org/10.1007/s00432-023-04599-0
_version_ 1785075318326820864
author Condic, Mateja
Neidhöfer, Claudio
Ralser, Damian J.
Wetzig, Nina
Thiele, Ralf
Sieber, Martin
Otten, Lucia A.
Warwas, Leonie K.
Hoerauf, Achim
Mustea, Alexander
Parčina, Marijo
author_facet Condic, Mateja
Neidhöfer, Claudio
Ralser, Damian J.
Wetzig, Nina
Thiele, Ralf
Sieber, Martin
Otten, Lucia A.
Warwas, Leonie K.
Hoerauf, Achim
Mustea, Alexander
Parčina, Marijo
author_sort Condic, Mateja
collection PubMed
description PURPOSE: Cervical cancer (CC) is caused by a persistent high-risk human papillomavirus (hrHPV) infection. The cervico-vaginal microbiome may influence the development of (pre)cancer lesions. Aim of the study was (i) to evaluate the new CC screening program in Germany for the detection of high-grade CC precursor lesions, and (ii) to elucidate the role of the cervico-vaginal microbiome and its potential impact on cervical dysplasia. METHODS: The microbiome of 310 patients referred to colposcopy was determined by amplicon sequencing and correlated with clinicopathological parameters. RESULTS: Most patients were referred for colposcopy due to a positive hrHPV result in two consecutive years combined with a normal PAP smear. In 2.1% of these cases, a CIN III lesion was detected. There was a significant positive association between the PAP stage and Lactobacillus vaginalis colonization and between the severity of CC precursor lesions and Ureaplasma parvum. CONCLUSION: In our cohort, the new cervical cancer screening program resulted in a low rate of additional CIN III detected. It is questionable whether these cases were only identified earlier with additional HPV testing before the appearance of cytological abnormalities, or the new screening program will truly increase the detection rate of CIN III in the long run. Colonization with U. parvum was associated with histological dysplastic lesions. Whether targeted therapy of this pathogen or optimization of the microbiome prevents dysplasia remains speculative. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00432-023-04599-0.
format Online
Article
Text
id pubmed-10356625
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Springer Berlin Heidelberg
record_format MEDLINE/PubMed
spelling pubmed-103566252023-07-21 Analysis of the cervical microbiome in women from the German national cervical cancer screening program Condic, Mateja Neidhöfer, Claudio Ralser, Damian J. Wetzig, Nina Thiele, Ralf Sieber, Martin Otten, Lucia A. Warwas, Leonie K. Hoerauf, Achim Mustea, Alexander Parčina, Marijo J Cancer Res Clin Oncol Research PURPOSE: Cervical cancer (CC) is caused by a persistent high-risk human papillomavirus (hrHPV) infection. The cervico-vaginal microbiome may influence the development of (pre)cancer lesions. Aim of the study was (i) to evaluate the new CC screening program in Germany for the detection of high-grade CC precursor lesions, and (ii) to elucidate the role of the cervico-vaginal microbiome and its potential impact on cervical dysplasia. METHODS: The microbiome of 310 patients referred to colposcopy was determined by amplicon sequencing and correlated with clinicopathological parameters. RESULTS: Most patients were referred for colposcopy due to a positive hrHPV result in two consecutive years combined with a normal PAP smear. In 2.1% of these cases, a CIN III lesion was detected. There was a significant positive association between the PAP stage and Lactobacillus vaginalis colonization and between the severity of CC precursor lesions and Ureaplasma parvum. CONCLUSION: In our cohort, the new cervical cancer screening program resulted in a low rate of additional CIN III detected. It is questionable whether these cases were only identified earlier with additional HPV testing before the appearance of cytological abnormalities, or the new screening program will truly increase the detection rate of CIN III in the long run. Colonization with U. parvum was associated with histological dysplastic lesions. Whether targeted therapy of this pathogen or optimization of the microbiome prevents dysplasia remains speculative. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00432-023-04599-0. Springer Berlin Heidelberg 2023-02-13 2023 /pmc/articles/PMC10356625/ /pubmed/36780053 http://dx.doi.org/10.1007/s00432-023-04599-0 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research
Condic, Mateja
Neidhöfer, Claudio
Ralser, Damian J.
Wetzig, Nina
Thiele, Ralf
Sieber, Martin
Otten, Lucia A.
Warwas, Leonie K.
Hoerauf, Achim
Mustea, Alexander
Parčina, Marijo
Analysis of the cervical microbiome in women from the German national cervical cancer screening program
title Analysis of the cervical microbiome in women from the German national cervical cancer screening program
title_full Analysis of the cervical microbiome in women from the German national cervical cancer screening program
title_fullStr Analysis of the cervical microbiome in women from the German national cervical cancer screening program
title_full_unstemmed Analysis of the cervical microbiome in women from the German national cervical cancer screening program
title_short Analysis of the cervical microbiome in women from the German national cervical cancer screening program
title_sort analysis of the cervical microbiome in women from the german national cervical cancer screening program
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10356625/
https://www.ncbi.nlm.nih.gov/pubmed/36780053
http://dx.doi.org/10.1007/s00432-023-04599-0
work_keys_str_mv AT condicmateja analysisofthecervicalmicrobiomeinwomenfromthegermannationalcervicalcancerscreeningprogram
AT neidhoferclaudio analysisofthecervicalmicrobiomeinwomenfromthegermannationalcervicalcancerscreeningprogram
AT ralserdamianj analysisofthecervicalmicrobiomeinwomenfromthegermannationalcervicalcancerscreeningprogram
AT wetzignina analysisofthecervicalmicrobiomeinwomenfromthegermannationalcervicalcancerscreeningprogram
AT thieleralf analysisofthecervicalmicrobiomeinwomenfromthegermannationalcervicalcancerscreeningprogram
AT siebermartin analysisofthecervicalmicrobiomeinwomenfromthegermannationalcervicalcancerscreeningprogram
AT ottenluciaa analysisofthecervicalmicrobiomeinwomenfromthegermannationalcervicalcancerscreeningprogram
AT warwasleoniek analysisofthecervicalmicrobiomeinwomenfromthegermannationalcervicalcancerscreeningprogram
AT hoeraufachim analysisofthecervicalmicrobiomeinwomenfromthegermannationalcervicalcancerscreeningprogram
AT musteaalexander analysisofthecervicalmicrobiomeinwomenfromthegermannationalcervicalcancerscreeningprogram
AT parcinamarijo analysisofthecervicalmicrobiomeinwomenfromthegermannationalcervicalcancerscreeningprogram