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Purkinje cell microzones mediate distinct kinematics of a single movement

The classification of neuronal subpopulations has significantly advanced, yet its relevance for behavior remains unclear. The highly organized flocculus of the cerebellum, known to fine-tune multi-axial eye movements, is an ideal substrate for the study of potential functions of neuronal subpopulati...

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Autores principales: Blot, François G. C., White, Joshua J., van Hattem, Amy, Scotti, Licia, Balaji, Vaishnavi, Adolfs, Youri, Pasterkamp, R. Jeroen, De Zeeuw, Chris I., Schonewille, Martijn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10356806/
https://www.ncbi.nlm.nih.gov/pubmed/37468512
http://dx.doi.org/10.1038/s41467-023-40111-5
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author Blot, François G. C.
White, Joshua J.
van Hattem, Amy
Scotti, Licia
Balaji, Vaishnavi
Adolfs, Youri
Pasterkamp, R. Jeroen
De Zeeuw, Chris I.
Schonewille, Martijn
author_facet Blot, François G. C.
White, Joshua J.
van Hattem, Amy
Scotti, Licia
Balaji, Vaishnavi
Adolfs, Youri
Pasterkamp, R. Jeroen
De Zeeuw, Chris I.
Schonewille, Martijn
author_sort Blot, François G. C.
collection PubMed
description The classification of neuronal subpopulations has significantly advanced, yet its relevance for behavior remains unclear. The highly organized flocculus of the cerebellum, known to fine-tune multi-axial eye movements, is an ideal substrate for the study of potential functions of neuronal subpopulations. Here, we demonstrate that its recently identified subpopulations of 9+ and 9- Purkinje cells exhibit an intermediate Aldolase C expression and electrophysiological profile, providing evidence for a graded continuum of intrinsic properties among PC subpopulations. By identifying and utilizing two Cre-lines that genetically target these floccular domains, we show with high spatial specificity that these subpopulations of Purkinje cells participate in separate micromodules with topographically organized connections. Finally, optogenetic excitation of the respective subpopulations results in movements around the same axis in space, yet with distinct kinematic profiles. These results indicate that Purkinje cell subpopulations integrate in discrete circuits and mediate particular parameters of single movements.
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spelling pubmed-103568062023-07-21 Purkinje cell microzones mediate distinct kinematics of a single movement Blot, François G. C. White, Joshua J. van Hattem, Amy Scotti, Licia Balaji, Vaishnavi Adolfs, Youri Pasterkamp, R. Jeroen De Zeeuw, Chris I. Schonewille, Martijn Nat Commun Article The classification of neuronal subpopulations has significantly advanced, yet its relevance for behavior remains unclear. The highly organized flocculus of the cerebellum, known to fine-tune multi-axial eye movements, is an ideal substrate for the study of potential functions of neuronal subpopulations. Here, we demonstrate that its recently identified subpopulations of 9+ and 9- Purkinje cells exhibit an intermediate Aldolase C expression and electrophysiological profile, providing evidence for a graded continuum of intrinsic properties among PC subpopulations. By identifying and utilizing two Cre-lines that genetically target these floccular domains, we show with high spatial specificity that these subpopulations of Purkinje cells participate in separate micromodules with topographically organized connections. Finally, optogenetic excitation of the respective subpopulations results in movements around the same axis in space, yet with distinct kinematic profiles. These results indicate that Purkinje cell subpopulations integrate in discrete circuits and mediate particular parameters of single movements. Nature Publishing Group UK 2023-07-19 /pmc/articles/PMC10356806/ /pubmed/37468512 http://dx.doi.org/10.1038/s41467-023-40111-5 Text en © The Author(s) 2023, corrected publication 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Blot, François G. C.
White, Joshua J.
van Hattem, Amy
Scotti, Licia
Balaji, Vaishnavi
Adolfs, Youri
Pasterkamp, R. Jeroen
De Zeeuw, Chris I.
Schonewille, Martijn
Purkinje cell microzones mediate distinct kinematics of a single movement
title Purkinje cell microzones mediate distinct kinematics of a single movement
title_full Purkinje cell microzones mediate distinct kinematics of a single movement
title_fullStr Purkinje cell microzones mediate distinct kinematics of a single movement
title_full_unstemmed Purkinje cell microzones mediate distinct kinematics of a single movement
title_short Purkinje cell microzones mediate distinct kinematics of a single movement
title_sort purkinje cell microzones mediate distinct kinematics of a single movement
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10356806/
https://www.ncbi.nlm.nih.gov/pubmed/37468512
http://dx.doi.org/10.1038/s41467-023-40111-5
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