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Dual-color DNA-PAINT single-particle tracking enables extended studies of membrane protein interactions
DNA-PAINT based single-particle tracking (DNA-PAINT-SPT) has recently significantly enhanced observation times in in vitro SPT experiments by overcoming the constraints of fluorophore photobleaching. However, with the reported implementation, only a single target can be imaged and the technique cann...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10356854/ https://www.ncbi.nlm.nih.gov/pubmed/37468504 http://dx.doi.org/10.1038/s41467-023-40065-8 |
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author | Niederauer, Christian Nguyen, Chikim Wang-Henderson, Miles Stein, Johannes Strauss, Sebastian Cumberworth, Alexander Stehr, Florian Jungmann, Ralf Schwille, Petra Ganzinger, Kristina A. |
author_facet | Niederauer, Christian Nguyen, Chikim Wang-Henderson, Miles Stein, Johannes Strauss, Sebastian Cumberworth, Alexander Stehr, Florian Jungmann, Ralf Schwille, Petra Ganzinger, Kristina A. |
author_sort | Niederauer, Christian |
collection | PubMed |
description | DNA-PAINT based single-particle tracking (DNA-PAINT-SPT) has recently significantly enhanced observation times in in vitro SPT experiments by overcoming the constraints of fluorophore photobleaching. However, with the reported implementation, only a single target can be imaged and the technique cannot be applied straight to live cell imaging. Here we report on leveraging this technique from a proof-of-principle implementation to a useful tool for the SPT community by introducing simultaneous live cell dual-color DNA-PAINT-SPT for quantifying protein dimerization and tracking proteins in living cell membranes, demonstrating its improved performance over single-dye SPT. |
format | Online Article Text |
id | pubmed-10356854 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-103568542023-07-21 Dual-color DNA-PAINT single-particle tracking enables extended studies of membrane protein interactions Niederauer, Christian Nguyen, Chikim Wang-Henderson, Miles Stein, Johannes Strauss, Sebastian Cumberworth, Alexander Stehr, Florian Jungmann, Ralf Schwille, Petra Ganzinger, Kristina A. Nat Commun Article DNA-PAINT based single-particle tracking (DNA-PAINT-SPT) has recently significantly enhanced observation times in in vitro SPT experiments by overcoming the constraints of fluorophore photobleaching. However, with the reported implementation, only a single target can be imaged and the technique cannot be applied straight to live cell imaging. Here we report on leveraging this technique from a proof-of-principle implementation to a useful tool for the SPT community by introducing simultaneous live cell dual-color DNA-PAINT-SPT for quantifying protein dimerization and tracking proteins in living cell membranes, demonstrating its improved performance over single-dye SPT. Nature Publishing Group UK 2023-07-19 /pmc/articles/PMC10356854/ /pubmed/37468504 http://dx.doi.org/10.1038/s41467-023-40065-8 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Niederauer, Christian Nguyen, Chikim Wang-Henderson, Miles Stein, Johannes Strauss, Sebastian Cumberworth, Alexander Stehr, Florian Jungmann, Ralf Schwille, Petra Ganzinger, Kristina A. Dual-color DNA-PAINT single-particle tracking enables extended studies of membrane protein interactions |
title | Dual-color DNA-PAINT single-particle tracking enables extended studies of membrane protein interactions |
title_full | Dual-color DNA-PAINT single-particle tracking enables extended studies of membrane protein interactions |
title_fullStr | Dual-color DNA-PAINT single-particle tracking enables extended studies of membrane protein interactions |
title_full_unstemmed | Dual-color DNA-PAINT single-particle tracking enables extended studies of membrane protein interactions |
title_short | Dual-color DNA-PAINT single-particle tracking enables extended studies of membrane protein interactions |
title_sort | dual-color dna-paint single-particle tracking enables extended studies of membrane protein interactions |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10356854/ https://www.ncbi.nlm.nih.gov/pubmed/37468504 http://dx.doi.org/10.1038/s41467-023-40065-8 |
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