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Quantification of N(τ) -Methylhistidine and N(π)-Methylhistidine in Chicken Plasma by Liquid Chromatography–Tandem Mass Spectrometry

The concentration of N(τ)-methylhistidine in plasma provides an index of skeletal muscle protein breakdown. This study aimed to establish a quantitative method for measuring the concentrations of N(τ)-methylhistidine and its isomer N(π)-methylhistidine in chicken plasma, using liquid chromatography–...

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Autores principales: Shiraishi, Jun-ichi, Ijiri, Daichi, Katafuchi, Ayumi, Tomonaga, Shozo, Shimamoto, Saki, Do, Hanwool, Ishihara, Shinya, Ohtsuka, Akira
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japan Poultry Science Association 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10357029/
https://www.ncbi.nlm.nih.gov/pubmed/37484878
http://dx.doi.org/10.2141/jpsa.2023017
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author Shiraishi, Jun-ichi
Ijiri, Daichi
Katafuchi, Ayumi
Tomonaga, Shozo
Shimamoto, Saki
Do, Hanwool
Ishihara, Shinya
Ohtsuka, Akira
author_facet Shiraishi, Jun-ichi
Ijiri, Daichi
Katafuchi, Ayumi
Tomonaga, Shozo
Shimamoto, Saki
Do, Hanwool
Ishihara, Shinya
Ohtsuka, Akira
author_sort Shiraishi, Jun-ichi
collection PubMed
description The concentration of N(τ)-methylhistidine in plasma provides an index of skeletal muscle protein breakdown. This study aimed to establish a quantitative method for measuring the concentrations of N(τ)-methylhistidine and its isomer N(π)-methylhistidine in chicken plasma, using liquid chromatography–tandem mass spectrometry with stable isotope dilution analysis. The acceptable linear ranges of detection were 1.56–50.00 μmol/L for N(τ)-methylhistidine and 0.78–25.00 μmol/L for N(π)-methylhistidine. The proposed method detected changes in the plasma levels of N(τ)-methylhistidine and N(π)-methylhistidine in response to fasting and re-feeding. These results suggest that the method developed in this study can be used for the simultaneous measurement of N(τ)-methylhistidine and N(π)-methylhistidine in chicken plasma.
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spelling pubmed-103570292023-07-22 Quantification of N(τ) -Methylhistidine and N(π)-Methylhistidine in Chicken Plasma by Liquid Chromatography–Tandem Mass Spectrometry Shiraishi, Jun-ichi Ijiri, Daichi Katafuchi, Ayumi Tomonaga, Shozo Shimamoto, Saki Do, Hanwool Ishihara, Shinya Ohtsuka, Akira J Poult Sci Full Paper The concentration of N(τ)-methylhistidine in plasma provides an index of skeletal muscle protein breakdown. This study aimed to establish a quantitative method for measuring the concentrations of N(τ)-methylhistidine and its isomer N(π)-methylhistidine in chicken plasma, using liquid chromatography–tandem mass spectrometry with stable isotope dilution analysis. The acceptable linear ranges of detection were 1.56–50.00 μmol/L for N(τ)-methylhistidine and 0.78–25.00 μmol/L for N(π)-methylhistidine. The proposed method detected changes in the plasma levels of N(τ)-methylhistidine and N(π)-methylhistidine in response to fasting and re-feeding. These results suggest that the method developed in this study can be used for the simultaneous measurement of N(τ)-methylhistidine and N(π)-methylhistidine in chicken plasma. Japan Poultry Science Association 2023-07-22 /pmc/articles/PMC10357029/ /pubmed/37484878 http://dx.doi.org/10.2141/jpsa.2023017 Text en 2023 Japan Poultry Science Association. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (CC BY-NC-ND) 4.0 License.
spellingShingle Full Paper
Shiraishi, Jun-ichi
Ijiri, Daichi
Katafuchi, Ayumi
Tomonaga, Shozo
Shimamoto, Saki
Do, Hanwool
Ishihara, Shinya
Ohtsuka, Akira
Quantification of N(τ) -Methylhistidine and N(π)-Methylhistidine in Chicken Plasma by Liquid Chromatography–Tandem Mass Spectrometry
title Quantification of N(τ) -Methylhistidine and N(π)-Methylhistidine in Chicken Plasma by Liquid Chromatography–Tandem Mass Spectrometry
title_full Quantification of N(τ) -Methylhistidine and N(π)-Methylhistidine in Chicken Plasma by Liquid Chromatography–Tandem Mass Spectrometry
title_fullStr Quantification of N(τ) -Methylhistidine and N(π)-Methylhistidine in Chicken Plasma by Liquid Chromatography–Tandem Mass Spectrometry
title_full_unstemmed Quantification of N(τ) -Methylhistidine and N(π)-Methylhistidine in Chicken Plasma by Liquid Chromatography–Tandem Mass Spectrometry
title_short Quantification of N(τ) -Methylhistidine and N(π)-Methylhistidine in Chicken Plasma by Liquid Chromatography–Tandem Mass Spectrometry
title_sort quantification of n(τ) -methylhistidine and n(π)-methylhistidine in chicken plasma by liquid chromatography–tandem mass spectrometry
topic Full Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10357029/
https://www.ncbi.nlm.nih.gov/pubmed/37484878
http://dx.doi.org/10.2141/jpsa.2023017
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