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Quantification of N(τ) -Methylhistidine and N(π)-Methylhistidine in Chicken Plasma by Liquid Chromatography–Tandem Mass Spectrometry
The concentration of N(τ)-methylhistidine in plasma provides an index of skeletal muscle protein breakdown. This study aimed to establish a quantitative method for measuring the concentrations of N(τ)-methylhistidine and its isomer N(π)-methylhistidine in chicken plasma, using liquid chromatography–...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Japan Poultry Science Association
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10357029/ https://www.ncbi.nlm.nih.gov/pubmed/37484878 http://dx.doi.org/10.2141/jpsa.2023017 |
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author | Shiraishi, Jun-ichi Ijiri, Daichi Katafuchi, Ayumi Tomonaga, Shozo Shimamoto, Saki Do, Hanwool Ishihara, Shinya Ohtsuka, Akira |
author_facet | Shiraishi, Jun-ichi Ijiri, Daichi Katafuchi, Ayumi Tomonaga, Shozo Shimamoto, Saki Do, Hanwool Ishihara, Shinya Ohtsuka, Akira |
author_sort | Shiraishi, Jun-ichi |
collection | PubMed |
description | The concentration of N(τ)-methylhistidine in plasma provides an index of skeletal muscle protein breakdown. This study aimed to establish a quantitative method for measuring the concentrations of N(τ)-methylhistidine and its isomer N(π)-methylhistidine in chicken plasma, using liquid chromatography–tandem mass spectrometry with stable isotope dilution analysis. The acceptable linear ranges of detection were 1.56–50.00 μmol/L for N(τ)-methylhistidine and 0.78–25.00 μmol/L for N(π)-methylhistidine. The proposed method detected changes in the plasma levels of N(τ)-methylhistidine and N(π)-methylhistidine in response to fasting and re-feeding. These results suggest that the method developed in this study can be used for the simultaneous measurement of N(τ)-methylhistidine and N(π)-methylhistidine in chicken plasma. |
format | Online Article Text |
id | pubmed-10357029 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Japan Poultry Science Association |
record_format | MEDLINE/PubMed |
spelling | pubmed-103570292023-07-22 Quantification of N(τ) -Methylhistidine and N(π)-Methylhistidine in Chicken Plasma by Liquid Chromatography–Tandem Mass Spectrometry Shiraishi, Jun-ichi Ijiri, Daichi Katafuchi, Ayumi Tomonaga, Shozo Shimamoto, Saki Do, Hanwool Ishihara, Shinya Ohtsuka, Akira J Poult Sci Full Paper The concentration of N(τ)-methylhistidine in plasma provides an index of skeletal muscle protein breakdown. This study aimed to establish a quantitative method for measuring the concentrations of N(τ)-methylhistidine and its isomer N(π)-methylhistidine in chicken plasma, using liquid chromatography–tandem mass spectrometry with stable isotope dilution analysis. The acceptable linear ranges of detection were 1.56–50.00 μmol/L for N(τ)-methylhistidine and 0.78–25.00 μmol/L for N(π)-methylhistidine. The proposed method detected changes in the plasma levels of N(τ)-methylhistidine and N(π)-methylhistidine in response to fasting and re-feeding. These results suggest that the method developed in this study can be used for the simultaneous measurement of N(τ)-methylhistidine and N(π)-methylhistidine in chicken plasma. Japan Poultry Science Association 2023-07-22 /pmc/articles/PMC10357029/ /pubmed/37484878 http://dx.doi.org/10.2141/jpsa.2023017 Text en 2023 Japan Poultry Science Association. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (CC BY-NC-ND) 4.0 License. |
spellingShingle | Full Paper Shiraishi, Jun-ichi Ijiri, Daichi Katafuchi, Ayumi Tomonaga, Shozo Shimamoto, Saki Do, Hanwool Ishihara, Shinya Ohtsuka, Akira Quantification of N(τ) -Methylhistidine and N(π)-Methylhistidine in Chicken Plasma by Liquid Chromatography–Tandem Mass Spectrometry |
title | Quantification of N(τ) -Methylhistidine and
N(π)-Methylhistidine in Chicken Plasma by Liquid Chromatography–Tandem Mass
Spectrometry |
title_full | Quantification of N(τ) -Methylhistidine and
N(π)-Methylhistidine in Chicken Plasma by Liquid Chromatography–Tandem Mass
Spectrometry |
title_fullStr | Quantification of N(τ) -Methylhistidine and
N(π)-Methylhistidine in Chicken Plasma by Liquid Chromatography–Tandem Mass
Spectrometry |
title_full_unstemmed | Quantification of N(τ) -Methylhistidine and
N(π)-Methylhistidine in Chicken Plasma by Liquid Chromatography–Tandem Mass
Spectrometry |
title_short | Quantification of N(τ) -Methylhistidine and
N(π)-Methylhistidine in Chicken Plasma by Liquid Chromatography–Tandem Mass
Spectrometry |
title_sort | quantification of n(τ) -methylhistidine and
n(π)-methylhistidine in chicken plasma by liquid chromatography–tandem mass
spectrometry |
topic | Full Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10357029/ https://www.ncbi.nlm.nih.gov/pubmed/37484878 http://dx.doi.org/10.2141/jpsa.2023017 |
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