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Elimination of aberrantly specified cell clones is independent of interfacial Myosin II accumulation

Spatial organization within an organ is essential and needs to be maintained during development. This is largely implemented via compartment boundaries that serve as barriers between distinct cell types. Biased accumulation of junctional non-muscle Myosin II along the interface between differently f...

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Autores principales: Klipa, Olga, El Gammal, Menna, Hamaratoglu, Fisun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Company of Biologists Ltd 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10357031/
https://www.ncbi.nlm.nih.gov/pubmed/37309190
http://dx.doi.org/10.1242/jcs.259935
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author Klipa, Olga
El Gammal, Menna
Hamaratoglu, Fisun
author_facet Klipa, Olga
El Gammal, Menna
Hamaratoglu, Fisun
author_sort Klipa, Olga
collection PubMed
description Spatial organization within an organ is essential and needs to be maintained during development. This is largely implemented via compartment boundaries that serve as barriers between distinct cell types. Biased accumulation of junctional non-muscle Myosin II along the interface between differently fated groups of cells contributes to boundary integrity and maintains its shape via increased tension. Here, using the Drosophila wing imaginal disc, we tested whether interfacial tension driven by accumulation of Myosin is responsible for the elimination of aberrantly specified cells that would otherwise compromise compartment organization. To this end, we genetically reduced Myosin II levels in three different patterns: in both wild-type and misspecified cells, only in misspecified cells, and specifically at the interface between wild-type and aberrantly specified cells. We found that the recognition and elimination of aberrantly specified cells do not strictly rely on tensile forces driven by interfacial Myosin cables. Moreover, apical constriction of misspecified cells and their separation from wild-type neighbours occurred even when Myosin levels were greatly reduced. Thus, we conclude that the forces that drive elimination of aberrantly specified cells are largely independent of Myosin II accumulation.
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spelling pubmed-103570312023-07-21 Elimination of aberrantly specified cell clones is independent of interfacial Myosin II accumulation Klipa, Olga El Gammal, Menna Hamaratoglu, Fisun J Cell Sci Research Article Spatial organization within an organ is essential and needs to be maintained during development. This is largely implemented via compartment boundaries that serve as barriers between distinct cell types. Biased accumulation of junctional non-muscle Myosin II along the interface between differently fated groups of cells contributes to boundary integrity and maintains its shape via increased tension. Here, using the Drosophila wing imaginal disc, we tested whether interfacial tension driven by accumulation of Myosin is responsible for the elimination of aberrantly specified cells that would otherwise compromise compartment organization. To this end, we genetically reduced Myosin II levels in three different patterns: in both wild-type and misspecified cells, only in misspecified cells, and specifically at the interface between wild-type and aberrantly specified cells. We found that the recognition and elimination of aberrantly specified cells do not strictly rely on tensile forces driven by interfacial Myosin cables. Moreover, apical constriction of misspecified cells and their separation from wild-type neighbours occurred even when Myosin levels were greatly reduced. Thus, we conclude that the forces that drive elimination of aberrantly specified cells are largely independent of Myosin II accumulation. The Company of Biologists Ltd 2023-07-11 /pmc/articles/PMC10357031/ /pubmed/37309190 http://dx.doi.org/10.1242/jcs.259935 Text en © 2023. Published by The Company of Biologists Ltd https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0 (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
spellingShingle Research Article
Klipa, Olga
El Gammal, Menna
Hamaratoglu, Fisun
Elimination of aberrantly specified cell clones is independent of interfacial Myosin II accumulation
title Elimination of aberrantly specified cell clones is independent of interfacial Myosin II accumulation
title_full Elimination of aberrantly specified cell clones is independent of interfacial Myosin II accumulation
title_fullStr Elimination of aberrantly specified cell clones is independent of interfacial Myosin II accumulation
title_full_unstemmed Elimination of aberrantly specified cell clones is independent of interfacial Myosin II accumulation
title_short Elimination of aberrantly specified cell clones is independent of interfacial Myosin II accumulation
title_sort elimination of aberrantly specified cell clones is independent of interfacial myosin ii accumulation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10357031/
https://www.ncbi.nlm.nih.gov/pubmed/37309190
http://dx.doi.org/10.1242/jcs.259935
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