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Novel blood typing method by discrimination of hemagglutination and rouleaux using an erythrocyte aggregometer

BACKGROUND: In pretransfusion blood typing, pretreatments such as centrifugation and suspension of red blood cells (RBCs) and mixing them with sufficient amounts of reagents are required, but these steps are time-consuming and costly. OBJECTIVE: Aiming to develop a new blood typing method that requi...

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Autores principales: Higuchi, Makoto, Sekiba, Yasuhiro, Watanabe, Nobuo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: IOS Press 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10357158/
https://www.ncbi.nlm.nih.gov/pubmed/37005880
http://dx.doi.org/10.3233/CH-221651
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author Higuchi, Makoto
Sekiba, Yasuhiro
Watanabe, Nobuo
author_facet Higuchi, Makoto
Sekiba, Yasuhiro
Watanabe, Nobuo
author_sort Higuchi, Makoto
collection PubMed
description BACKGROUND: In pretransfusion blood typing, pretreatments such as centrifugation and suspension of red blood cells (RBCs) and mixing them with sufficient amounts of reagents are required, but these steps are time-consuming and costly. OBJECTIVE: Aiming to develop a new blood typing method that requires no dilution and only a small amount of reagent, we attempted to determine blood type using syllectometry, an easy-to-use and rapid optical method for measuring the RBC aggregation that occurs when blood flow is abruptly stopped in a flow channel. METHODS: Samples of whole blood from 20 healthy participants were mixed with antibody reagents for blood typing at mixing ratios of 2.5% to 10% and measured with a syllectometry device. RESULTS: Amplitude (AMP), one of the aggregation parameters, showed significant differences between agglutination and non-agglutination samples at mixing ratios from 2.5% to 10%. Although there were significant individual differences in aggregation parameters, calculation of AMP relative to that of blood before reagent mixing reduced the individual differences and enabled determination of blood type in all participants. CONCLUSIONS: This new method enables blood typing with a small amount of reagent, without the time-consuming and labor-intensive pretreatments such as centrifugation and suspension of RBCs.
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spelling pubmed-103571582023-07-21 Novel blood typing method by discrimination of hemagglutination and rouleaux using an erythrocyte aggregometer Higuchi, Makoto Sekiba, Yasuhiro Watanabe, Nobuo Clin Hemorheol Microcirc Research Article BACKGROUND: In pretransfusion blood typing, pretreatments such as centrifugation and suspension of red blood cells (RBCs) and mixing them with sufficient amounts of reagents are required, but these steps are time-consuming and costly. OBJECTIVE: Aiming to develop a new blood typing method that requires no dilution and only a small amount of reagent, we attempted to determine blood type using syllectometry, an easy-to-use and rapid optical method for measuring the RBC aggregation that occurs when blood flow is abruptly stopped in a flow channel. METHODS: Samples of whole blood from 20 healthy participants were mixed with antibody reagents for blood typing at mixing ratios of 2.5% to 10% and measured with a syllectometry device. RESULTS: Amplitude (AMP), one of the aggregation parameters, showed significant differences between agglutination and non-agglutination samples at mixing ratios from 2.5% to 10%. Although there were significant individual differences in aggregation parameters, calculation of AMP relative to that of blood before reagent mixing reduced the individual differences and enabled determination of blood type in all participants. CONCLUSIONS: This new method enables blood typing with a small amount of reagent, without the time-consuming and labor-intensive pretreatments such as centrifugation and suspension of RBCs. IOS Press 2023-06-20 /pmc/articles/PMC10357158/ /pubmed/37005880 http://dx.doi.org/10.3233/CH-221651 Text en © 2023 – The authors. Published by IOS Press https://creativecommons.org/licenses/by-nc/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial (CC BY-NC 4.0) License (https://creativecommons.org/licenses/by-nc/4.0/) , which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Higuchi, Makoto
Sekiba, Yasuhiro
Watanabe, Nobuo
Novel blood typing method by discrimination of hemagglutination and rouleaux using an erythrocyte aggregometer
title Novel blood typing method by discrimination of hemagglutination and rouleaux using an erythrocyte aggregometer
title_full Novel blood typing method by discrimination of hemagglutination and rouleaux using an erythrocyte aggregometer
title_fullStr Novel blood typing method by discrimination of hemagglutination and rouleaux using an erythrocyte aggregometer
title_full_unstemmed Novel blood typing method by discrimination of hemagglutination and rouleaux using an erythrocyte aggregometer
title_short Novel blood typing method by discrimination of hemagglutination and rouleaux using an erythrocyte aggregometer
title_sort novel blood typing method by discrimination of hemagglutination and rouleaux using an erythrocyte aggregometer
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10357158/
https://www.ncbi.nlm.nih.gov/pubmed/37005880
http://dx.doi.org/10.3233/CH-221651
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