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Sensitive detection of lipopolysaccharides by monitoring of interleukin-10 secretion from human PBMCs

Lipopolysaccharide (LPS) contaminations may falsify immunological experiments and are crucial for pharmaceutical products because they cause life-threatening immune reactions. Here, we present interleukin-10 (IL-10) as a reliable marker to measure LPS contents when the readout of pro-inflammatory cy...

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Autores principales: Ivanusic, Daniel, Denner, Joachim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10357324/
https://www.ncbi.nlm.nih.gov/pubmed/37485021
http://dx.doi.org/10.17912/micropub.biology.000773
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author Ivanusic, Daniel
Denner, Joachim
author_facet Ivanusic, Daniel
Denner, Joachim
author_sort Ivanusic, Daniel
collection PubMed
description Lipopolysaccharide (LPS) contaminations may falsify immunological experiments and are crucial for pharmaceutical products because they cause life-threatening immune reactions. Here, we present interleukin-10 (IL-10) as a reliable marker to measure LPS contents when the readout of pro-inflammatory cytokines is not favored. This animal free source assay is able to detect LPS with a limit of detection (LOD) of 0.024 EU/ml by monitoring IL-10 secretions from isolated human peripheral blood mononuclear cells (PBMCs).
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spelling pubmed-103573242023-07-21 Sensitive detection of lipopolysaccharides by monitoring of interleukin-10 secretion from human PBMCs Ivanusic, Daniel Denner, Joachim MicroPubl Biol New Methods Lipopolysaccharide (LPS) contaminations may falsify immunological experiments and are crucial for pharmaceutical products because they cause life-threatening immune reactions. Here, we present interleukin-10 (IL-10) as a reliable marker to measure LPS contents when the readout of pro-inflammatory cytokines is not favored. This animal free source assay is able to detect LPS with a limit of detection (LOD) of 0.024 EU/ml by monitoring IL-10 secretions from isolated human peripheral blood mononuclear cells (PBMCs). Caltech Library 2023-07-05 /pmc/articles/PMC10357324/ /pubmed/37485021 http://dx.doi.org/10.17912/micropub.biology.000773 Text en Copyright: © 2023 by the authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle New Methods
Ivanusic, Daniel
Denner, Joachim
Sensitive detection of lipopolysaccharides by monitoring of interleukin-10 secretion from human PBMCs
title Sensitive detection of lipopolysaccharides by monitoring of interleukin-10 secretion from human PBMCs
title_full Sensitive detection of lipopolysaccharides by monitoring of interleukin-10 secretion from human PBMCs
title_fullStr Sensitive detection of lipopolysaccharides by monitoring of interleukin-10 secretion from human PBMCs
title_full_unstemmed Sensitive detection of lipopolysaccharides by monitoring of interleukin-10 secretion from human PBMCs
title_short Sensitive detection of lipopolysaccharides by monitoring of interleukin-10 secretion from human PBMCs
title_sort sensitive detection of lipopolysaccharides by monitoring of interleukin-10 secretion from human pbmcs
topic New Methods
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10357324/
https://www.ncbi.nlm.nih.gov/pubmed/37485021
http://dx.doi.org/10.17912/micropub.biology.000773
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