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Deep eutectic solvent-assisted fabrication of bioinspired 3D carbon–calcium phosphate scaffolds for bone tissue engineering

Tissue engineering is a burgeoning field focused on repairing damaged tissues through the combination of bodily cells with highly porous scaffold biomaterials, which serve as templates for tissue regeneration, thus facilitating the growth of new tissue. Carbon materials, constituting an emerging cla...

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Autores principales: Wysokowski, Marcin, Machałowski, Tomasz, Idaszek, Joanna, Chlanda, Adrian, Jaroszewicz, Jakub, Heljak, Marcin, Niemczak, Michał, Piasecki, Adam, Gajewska, Marta, Ehrlich, Hermann, Święszkowski, Wojciech, Jesionowski, Teofil
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10358318/
https://www.ncbi.nlm.nih.gov/pubmed/37483675
http://dx.doi.org/10.1039/d3ra02356g
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author Wysokowski, Marcin
Machałowski, Tomasz
Idaszek, Joanna
Chlanda, Adrian
Jaroszewicz, Jakub
Heljak, Marcin
Niemczak, Michał
Piasecki, Adam
Gajewska, Marta
Ehrlich, Hermann
Święszkowski, Wojciech
Jesionowski, Teofil
author_facet Wysokowski, Marcin
Machałowski, Tomasz
Idaszek, Joanna
Chlanda, Adrian
Jaroszewicz, Jakub
Heljak, Marcin
Niemczak, Michał
Piasecki, Adam
Gajewska, Marta
Ehrlich, Hermann
Święszkowski, Wojciech
Jesionowski, Teofil
author_sort Wysokowski, Marcin
collection PubMed
description Tissue engineering is a burgeoning field focused on repairing damaged tissues through the combination of bodily cells with highly porous scaffold biomaterials, which serve as templates for tissue regeneration, thus facilitating the growth of new tissue. Carbon materials, constituting an emerging class of superior materials, are currently experiencing remarkable scientific and technological advancements. Consequently, the development of novel 3D carbon-based composite materials has become significant for biomedicine. There is an urgent need for the development of hybrids that will combine the unique bioactivity of ceramics with the performance of carbonaceous materials. Considering these requirements, herein, we propose a straightforward method of producing a 3D carbon-based scaffold that resembles the structural features of spongin, even on the nanometric level of their hierarchical organization. The modification of spongin with calcium phosphate was achieved in a deep eutectic solvent (choline chloride : urea, 1 : 2). The holistic characterization of the scaffolds confirms their remarkable structural features (i.e., porosity, connectivity), along with the biocompatibility of α-tricalcium phosphate (α-TCP), rendering them a promising candidate for stem cell-based tissue-engineering. Culturing human bone marrow mesenchymal stem cells (hMSC) on the surface of the biomimetic scaffold further verifies its growth-facilitating properties, promoting the differentiation of these cells in the osteogenesis direction. ALP activity was significantly higher in osteogenic medium compared to proliferation, indicating the differentiation of hMSC towards osteoblasts. However, no significant difference between C and C–αTCP in the same medium type was observed.
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spelling pubmed-103583182023-07-21 Deep eutectic solvent-assisted fabrication of bioinspired 3D carbon–calcium phosphate scaffolds for bone tissue engineering Wysokowski, Marcin Machałowski, Tomasz Idaszek, Joanna Chlanda, Adrian Jaroszewicz, Jakub Heljak, Marcin Niemczak, Michał Piasecki, Adam Gajewska, Marta Ehrlich, Hermann Święszkowski, Wojciech Jesionowski, Teofil RSC Adv Chemistry Tissue engineering is a burgeoning field focused on repairing damaged tissues through the combination of bodily cells with highly porous scaffold biomaterials, which serve as templates for tissue regeneration, thus facilitating the growth of new tissue. Carbon materials, constituting an emerging class of superior materials, are currently experiencing remarkable scientific and technological advancements. Consequently, the development of novel 3D carbon-based composite materials has become significant for biomedicine. There is an urgent need for the development of hybrids that will combine the unique bioactivity of ceramics with the performance of carbonaceous materials. Considering these requirements, herein, we propose a straightforward method of producing a 3D carbon-based scaffold that resembles the structural features of spongin, even on the nanometric level of their hierarchical organization. The modification of spongin with calcium phosphate was achieved in a deep eutectic solvent (choline chloride : urea, 1 : 2). The holistic characterization of the scaffolds confirms their remarkable structural features (i.e., porosity, connectivity), along with the biocompatibility of α-tricalcium phosphate (α-TCP), rendering them a promising candidate for stem cell-based tissue-engineering. Culturing human bone marrow mesenchymal stem cells (hMSC) on the surface of the biomimetic scaffold further verifies its growth-facilitating properties, promoting the differentiation of these cells in the osteogenesis direction. ALP activity was significantly higher in osteogenic medium compared to proliferation, indicating the differentiation of hMSC towards osteoblasts. However, no significant difference between C and C–αTCP in the same medium type was observed. The Royal Society of Chemistry 2023-07-20 /pmc/articles/PMC10358318/ /pubmed/37483675 http://dx.doi.org/10.1039/d3ra02356g Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/
spellingShingle Chemistry
Wysokowski, Marcin
Machałowski, Tomasz
Idaszek, Joanna
Chlanda, Adrian
Jaroszewicz, Jakub
Heljak, Marcin
Niemczak, Michał
Piasecki, Adam
Gajewska, Marta
Ehrlich, Hermann
Święszkowski, Wojciech
Jesionowski, Teofil
Deep eutectic solvent-assisted fabrication of bioinspired 3D carbon–calcium phosphate scaffolds for bone tissue engineering
title Deep eutectic solvent-assisted fabrication of bioinspired 3D carbon–calcium phosphate scaffolds for bone tissue engineering
title_full Deep eutectic solvent-assisted fabrication of bioinspired 3D carbon–calcium phosphate scaffolds for bone tissue engineering
title_fullStr Deep eutectic solvent-assisted fabrication of bioinspired 3D carbon–calcium phosphate scaffolds for bone tissue engineering
title_full_unstemmed Deep eutectic solvent-assisted fabrication of bioinspired 3D carbon–calcium phosphate scaffolds for bone tissue engineering
title_short Deep eutectic solvent-assisted fabrication of bioinspired 3D carbon–calcium phosphate scaffolds for bone tissue engineering
title_sort deep eutectic solvent-assisted fabrication of bioinspired 3d carbon–calcium phosphate scaffolds for bone tissue engineering
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10358318/
https://www.ncbi.nlm.nih.gov/pubmed/37483675
http://dx.doi.org/10.1039/d3ra02356g
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