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Proline-conditioning and chemically-programmed ice nucleation protects spheroids during cryopreservation

Spheroids mimic 3-D tissue niches better than standard cell cultures. Cryopreserving spheroids, however, remains challenging as conventional cryoprotectants do not mitigate all damage mechanisms. Here chemically-programmed extracellular ice nucleation is used to prevent supercooling, alongside proli...

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Detalles Bibliográficos
Autores principales:	Gao, Yanan, Bissoyi, Akalabya, Kinney, Nina L. H., Whale, Thomas F., Guo, Qiongyu, Gibson, Matthew I.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	The Royal Society of Chemistry 2023
Materias:	Chemistry
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10358716/ https://www.ncbi.nlm.nih.gov/pubmed/37401839 http://dx.doi.org/10.1039/d3cc02252h

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