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Mining of key genes for cold adaptation from Pseudomonas fragi D12 and analysis of its cold-adaptation mechanism

The psychrotroph Pseudomonas fragi D12, which grew strongly under low temperatures, was screened from tundra soil collected from the permanent alpine zone on Changbai Mountain. To mine the genes critical for cold tolerance and to investigate the cold-adaptation mechanism, whole-genome sequencing, co...

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Autores principales: Bao, Changjie, Li, Muzi, Zhao, Xuhui, Shi, Jia, Liu, Yehui, Zhang, Na, Zhou, Yuqi, Ma, Jie, Chen, Guang, Zhang, Sitong, Chen, Huan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10358777/
https://www.ncbi.nlm.nih.gov/pubmed/37485517
http://dx.doi.org/10.3389/fmicb.2023.1215837
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author Bao, Changjie
Li, Muzi
Zhao, Xuhui
Shi, Jia
Liu, Yehui
Zhang, Na
Zhou, Yuqi
Ma, Jie
Chen, Guang
Zhang, Sitong
Chen, Huan
author_facet Bao, Changjie
Li, Muzi
Zhao, Xuhui
Shi, Jia
Liu, Yehui
Zhang, Na
Zhou, Yuqi
Ma, Jie
Chen, Guang
Zhang, Sitong
Chen, Huan
author_sort Bao, Changjie
collection PubMed
description The psychrotroph Pseudomonas fragi D12, which grew strongly under low temperatures, was screened from tundra soil collected from the permanent alpine zone on Changbai Mountain. To mine the genes critical for cold tolerance and to investigate the cold-adaptation mechanism, whole-genome sequencing, comparative genomic analysis, and transcriptome analysis were performed with P. fragi. A total of 124 potential cold adaptation genes were identified, including nineteen unique cold-adaptive genes were detected in the genome of P. fragi D12. Three unique genes associated with pili protein were significantly upregulated at different degrees of low temperature, which may be the key to the strong low-temperature adaptability of P. fragi D12. Meanwhile, we were pleasantly surprised to find that Pseudomonas fragi D12 exhibited different cold-adaptation mechanisms under different temperature changes. When the temperature declined from 30°C to 15°C, the response included maintenance of the fluidity of cell membranes, increased production of extracellular polymers, elevation in the content of compatibility solutes, and reduction in the content of reactive oxygen species, thereby providing a stable metabolic environment. When the temperature decreased from 15°C to 4°C, the response mainly included increases in the expression of molecular chaperones and transcription factors, enabling the bacteria to restore normal transcription and translation. The response mechanism of P. fragi D12 to low-temperature exposure is discussed. The results provide new ideas for the cold-adaptation mechanism of cold-tolerant microorganisms.
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spelling pubmed-103587772023-07-21 Mining of key genes for cold adaptation from Pseudomonas fragi D12 and analysis of its cold-adaptation mechanism Bao, Changjie Li, Muzi Zhao, Xuhui Shi, Jia Liu, Yehui Zhang, Na Zhou, Yuqi Ma, Jie Chen, Guang Zhang, Sitong Chen, Huan Front Microbiol Microbiology The psychrotroph Pseudomonas fragi D12, which grew strongly under low temperatures, was screened from tundra soil collected from the permanent alpine zone on Changbai Mountain. To mine the genes critical for cold tolerance and to investigate the cold-adaptation mechanism, whole-genome sequencing, comparative genomic analysis, and transcriptome analysis were performed with P. fragi. A total of 124 potential cold adaptation genes were identified, including nineteen unique cold-adaptive genes were detected in the genome of P. fragi D12. Three unique genes associated with pili protein were significantly upregulated at different degrees of low temperature, which may be the key to the strong low-temperature adaptability of P. fragi D12. Meanwhile, we were pleasantly surprised to find that Pseudomonas fragi D12 exhibited different cold-adaptation mechanisms under different temperature changes. When the temperature declined from 30°C to 15°C, the response included maintenance of the fluidity of cell membranes, increased production of extracellular polymers, elevation in the content of compatibility solutes, and reduction in the content of reactive oxygen species, thereby providing a stable metabolic environment. When the temperature decreased from 15°C to 4°C, the response mainly included increases in the expression of molecular chaperones and transcription factors, enabling the bacteria to restore normal transcription and translation. The response mechanism of P. fragi D12 to low-temperature exposure is discussed. The results provide new ideas for the cold-adaptation mechanism of cold-tolerant microorganisms. Frontiers Media S.A. 2023-07-06 /pmc/articles/PMC10358777/ /pubmed/37485517 http://dx.doi.org/10.3389/fmicb.2023.1215837 Text en Copyright © 2023 Bao, Li, Zhao, Shi, Liu, Zhang, Zhou, Ma, Chen, Zhang and Chen. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Bao, Changjie
Li, Muzi
Zhao, Xuhui
Shi, Jia
Liu, Yehui
Zhang, Na
Zhou, Yuqi
Ma, Jie
Chen, Guang
Zhang, Sitong
Chen, Huan
Mining of key genes for cold adaptation from Pseudomonas fragi D12 and analysis of its cold-adaptation mechanism
title Mining of key genes for cold adaptation from Pseudomonas fragi D12 and analysis of its cold-adaptation mechanism
title_full Mining of key genes for cold adaptation from Pseudomonas fragi D12 and analysis of its cold-adaptation mechanism
title_fullStr Mining of key genes for cold adaptation from Pseudomonas fragi D12 and analysis of its cold-adaptation mechanism
title_full_unstemmed Mining of key genes for cold adaptation from Pseudomonas fragi D12 and analysis of its cold-adaptation mechanism
title_short Mining of key genes for cold adaptation from Pseudomonas fragi D12 and analysis of its cold-adaptation mechanism
title_sort mining of key genes for cold adaptation from pseudomonas fragi d12 and analysis of its cold-adaptation mechanism
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10358777/
https://www.ncbi.nlm.nih.gov/pubmed/37485517
http://dx.doi.org/10.3389/fmicb.2023.1215837
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