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Simultaneous molecular detection of Anaplasma marginale and Theileria annulata in cattle blood samples collected from Pakistan-Afghanistan boarder region

Theileria annulata (T. annulata) and Anaplasma marginale (A. marginale) are among the most extensively reported tick borne pathogens and are associated with huge economic losses worldwide. A total of 298 cattle blood samples were screened to report the presence of these two pathogens. The samples we...

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Autores principales: Jamil, Sania, Chiou, Chien-Chun, Muqaddas, Hira, Ullah, Hayat, Asif, Muhammad, Rao, Sana, Hussain, Hafsa, Fatima, Qandeel, Nasreen, Nasreen, Niaz, Sadaf, Dzul-Rosado, Karla, Khan, Adil, Iqbal, Furhan, Chen, Chien-Chin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10358909/
https://www.ncbi.nlm.nih.gov/pubmed/37471404
http://dx.doi.org/10.1371/journal.pone.0288050
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author Jamil, Sania
Chiou, Chien-Chun
Muqaddas, Hira
Ullah, Hayat
Asif, Muhammad
Rao, Sana
Hussain, Hafsa
Fatima, Qandeel
Nasreen, Nasreen
Niaz, Sadaf
Dzul-Rosado, Karla
Khan, Adil
Iqbal, Furhan
Chen, Chien-Chin
author_facet Jamil, Sania
Chiou, Chien-Chun
Muqaddas, Hira
Ullah, Hayat
Asif, Muhammad
Rao, Sana
Hussain, Hafsa
Fatima, Qandeel
Nasreen, Nasreen
Niaz, Sadaf
Dzul-Rosado, Karla
Khan, Adil
Iqbal, Furhan
Chen, Chien-Chin
author_sort Jamil, Sania
collection PubMed
description Theileria annulata (T. annulata) and Anaplasma marginale (A. marginale) are among the most extensively reported tick borne pathogens and are associated with huge economic losses worldwide. A total of 298 cattle blood samples were screened to report the presence of these two pathogens. The samples were collected from apparently healthy cattle (Achai, n = 155, Jersy, n = 88 and crossbred, n = 55) in Bajaur district of Khyber Pakhtunkhwa (KPK) during June and July of 2022. A total of 31 out of 298 cattle (10.4%) were found infected with T. annulata as PCR amplified a 156 base pair fragment from Tams-1 gene of T. annulata from their blood. While 16/298 animals (5.4%) were found infected with A. marginale as they amplified a 382 base pair fragment specific for msp5 gene of this bacterium. Three animals (1%) were found co infected. Cattle susceptibility to T. annulata infection was significantly higher than A. marginale infection (P < 0.001). Phylogenetic analysis revealed that Pakistani isolates of both detected pathogen clustered together and were closely related isolates from worldwide countries. Prevalence of T. annulata varied significantly among the sampling sites (P = 0.05) while no such association was observed for A. marginale among the tested cattle. Epidemiological data analysis revealed that none of the studied risk factors was found associated either with the prevalence of T. annulata or A. marginale (P > 0.05) among enrolled cattle. In conclusion, our study has revealed a relatively higher prevalence of T. annulata than A. marginale in cattle from the Bajaur district in KPK. This information is important for improving the productivity of the livestock sector, which is one of the main sources of income in the country. It is recommended that this data be taken into account for the development and implementation of effective tick control programs, as well as for the improvement of livestock management practices to prevent and manage TBDs in Pakistan.
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spelling pubmed-103589092023-07-21 Simultaneous molecular detection of Anaplasma marginale and Theileria annulata in cattle blood samples collected from Pakistan-Afghanistan boarder region Jamil, Sania Chiou, Chien-Chun Muqaddas, Hira Ullah, Hayat Asif, Muhammad Rao, Sana Hussain, Hafsa Fatima, Qandeel Nasreen, Nasreen Niaz, Sadaf Dzul-Rosado, Karla Khan, Adil Iqbal, Furhan Chen, Chien-Chin PLoS One Research Article Theileria annulata (T. annulata) and Anaplasma marginale (A. marginale) are among the most extensively reported tick borne pathogens and are associated with huge economic losses worldwide. A total of 298 cattle blood samples were screened to report the presence of these two pathogens. The samples were collected from apparently healthy cattle (Achai, n = 155, Jersy, n = 88 and crossbred, n = 55) in Bajaur district of Khyber Pakhtunkhwa (KPK) during June and July of 2022. A total of 31 out of 298 cattle (10.4%) were found infected with T. annulata as PCR amplified a 156 base pair fragment from Tams-1 gene of T. annulata from their blood. While 16/298 animals (5.4%) were found infected with A. marginale as they amplified a 382 base pair fragment specific for msp5 gene of this bacterium. Three animals (1%) were found co infected. Cattle susceptibility to T. annulata infection was significantly higher than A. marginale infection (P < 0.001). Phylogenetic analysis revealed that Pakistani isolates of both detected pathogen clustered together and were closely related isolates from worldwide countries. Prevalence of T. annulata varied significantly among the sampling sites (P = 0.05) while no such association was observed for A. marginale among the tested cattle. Epidemiological data analysis revealed that none of the studied risk factors was found associated either with the prevalence of T. annulata or A. marginale (P > 0.05) among enrolled cattle. In conclusion, our study has revealed a relatively higher prevalence of T. annulata than A. marginale in cattle from the Bajaur district in KPK. This information is important for improving the productivity of the livestock sector, which is one of the main sources of income in the country. It is recommended that this data be taken into account for the development and implementation of effective tick control programs, as well as for the improvement of livestock management practices to prevent and manage TBDs in Pakistan. Public Library of Science 2023-07-20 /pmc/articles/PMC10358909/ /pubmed/37471404 http://dx.doi.org/10.1371/journal.pone.0288050 Text en © 2023 Jamil et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Jamil, Sania
Chiou, Chien-Chun
Muqaddas, Hira
Ullah, Hayat
Asif, Muhammad
Rao, Sana
Hussain, Hafsa
Fatima, Qandeel
Nasreen, Nasreen
Niaz, Sadaf
Dzul-Rosado, Karla
Khan, Adil
Iqbal, Furhan
Chen, Chien-Chin
Simultaneous molecular detection of Anaplasma marginale and Theileria annulata in cattle blood samples collected from Pakistan-Afghanistan boarder region
title Simultaneous molecular detection of Anaplasma marginale and Theileria annulata in cattle blood samples collected from Pakistan-Afghanistan boarder region
title_full Simultaneous molecular detection of Anaplasma marginale and Theileria annulata in cattle blood samples collected from Pakistan-Afghanistan boarder region
title_fullStr Simultaneous molecular detection of Anaplasma marginale and Theileria annulata in cattle blood samples collected from Pakistan-Afghanistan boarder region
title_full_unstemmed Simultaneous molecular detection of Anaplasma marginale and Theileria annulata in cattle blood samples collected from Pakistan-Afghanistan boarder region
title_short Simultaneous molecular detection of Anaplasma marginale and Theileria annulata in cattle blood samples collected from Pakistan-Afghanistan boarder region
title_sort simultaneous molecular detection of anaplasma marginale and theileria annulata in cattle blood samples collected from pakistan-afghanistan boarder region
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10358909/
https://www.ncbi.nlm.nih.gov/pubmed/37471404
http://dx.doi.org/10.1371/journal.pone.0288050
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