Cargando…

circSLC4A7 accelerates stemness and progression of gastric cancer by interacting with HSP90 to activate NOTCH1 signaling pathway

Gastric cancer stem cells (GCSCs) play critical roles in gastric cancer (GC) initiation and development. Circular RNAs (circRNAs) participate in diverse cancer biological processes and function as tumor suppressors or oncogenes. This study aims to discover the expression profile and functional roles...

Descripción completa

Detalles Bibliográficos
Autores principales: Hui, Yang, Wenguang, Yuan, Wei, Shang, Haoran, Wang, Shanglei, Ning, Ju, Liu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10359325/
https://www.ncbi.nlm.nih.gov/pubmed/37474578
http://dx.doi.org/10.1038/s41419-023-05976-w
_version_ 1785075856005136384
author Hui, Yang
Wenguang, Yuan
Wei, Shang
Haoran, Wang
Shanglei, Ning
Ju, Liu
author_facet Hui, Yang
Wenguang, Yuan
Wei, Shang
Haoran, Wang
Shanglei, Ning
Ju, Liu
author_sort Hui, Yang
collection PubMed
description Gastric cancer stem cells (GCSCs) play critical roles in gastric cancer (GC) initiation and development. Circular RNAs (circRNAs) participate in diverse cancer biological processes and function as tumor suppressors or oncogenes. This study aims to discover the expression profile and functional roles of circRNAs in GCSCs. A spheroid formation assay was conducted to enrich GCSCs. Genome-wide sequencing of circRNAs showed that a novel circRNA, circSLC4A7, was one of the most upregulated circRNAs in GCSCs. CircSLC4A7 was localized to the nucleus, and its level was elevated in GC cells and tissues. Furthermore, circSLC4A7 increased CSC-like properties and drove cell proliferation, migration, and invasion, which were determined by gain- and loss-of-function experiments. Specific circRNA pull-down assays followed by mass spectrometry analysis, RNA immunoprecipitation, and dual RNA-fluorescence in situ hybridization and immunofluorescence assay were conducted and HSP90 was detected to interact with circSLC4A7 and mediate the oncogenic function of circSLC4A7 by activating the Notch1 signaling pathway in GC. This study highlights a novel oncogenic function of circSLC4A7 mediated by its binding with HSP90 and thus activating the Notch1 signaling pathway.
format Online
Article
Text
id pubmed-10359325
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-103593252023-07-22 circSLC4A7 accelerates stemness and progression of gastric cancer by interacting with HSP90 to activate NOTCH1 signaling pathway Hui, Yang Wenguang, Yuan Wei, Shang Haoran, Wang Shanglei, Ning Ju, Liu Cell Death Dis Article Gastric cancer stem cells (GCSCs) play critical roles in gastric cancer (GC) initiation and development. Circular RNAs (circRNAs) participate in diverse cancer biological processes and function as tumor suppressors or oncogenes. This study aims to discover the expression profile and functional roles of circRNAs in GCSCs. A spheroid formation assay was conducted to enrich GCSCs. Genome-wide sequencing of circRNAs showed that a novel circRNA, circSLC4A7, was one of the most upregulated circRNAs in GCSCs. CircSLC4A7 was localized to the nucleus, and its level was elevated in GC cells and tissues. Furthermore, circSLC4A7 increased CSC-like properties and drove cell proliferation, migration, and invasion, which were determined by gain- and loss-of-function experiments. Specific circRNA pull-down assays followed by mass spectrometry analysis, RNA immunoprecipitation, and dual RNA-fluorescence in situ hybridization and immunofluorescence assay were conducted and HSP90 was detected to interact with circSLC4A7 and mediate the oncogenic function of circSLC4A7 by activating the Notch1 signaling pathway in GC. This study highlights a novel oncogenic function of circSLC4A7 mediated by its binding with HSP90 and thus activating the Notch1 signaling pathway. Nature Publishing Group UK 2023-07-20 /pmc/articles/PMC10359325/ /pubmed/37474578 http://dx.doi.org/10.1038/s41419-023-05976-w Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Hui, Yang
Wenguang, Yuan
Wei, Shang
Haoran, Wang
Shanglei, Ning
Ju, Liu
circSLC4A7 accelerates stemness and progression of gastric cancer by interacting with HSP90 to activate NOTCH1 signaling pathway
title circSLC4A7 accelerates stemness and progression of gastric cancer by interacting with HSP90 to activate NOTCH1 signaling pathway
title_full circSLC4A7 accelerates stemness and progression of gastric cancer by interacting with HSP90 to activate NOTCH1 signaling pathway
title_fullStr circSLC4A7 accelerates stemness and progression of gastric cancer by interacting with HSP90 to activate NOTCH1 signaling pathway
title_full_unstemmed circSLC4A7 accelerates stemness and progression of gastric cancer by interacting with HSP90 to activate NOTCH1 signaling pathway
title_short circSLC4A7 accelerates stemness and progression of gastric cancer by interacting with HSP90 to activate NOTCH1 signaling pathway
title_sort circslc4a7 accelerates stemness and progression of gastric cancer by interacting with hsp90 to activate notch1 signaling pathway
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10359325/
https://www.ncbi.nlm.nih.gov/pubmed/37474578
http://dx.doi.org/10.1038/s41419-023-05976-w
work_keys_str_mv AT huiyang circslc4a7acceleratesstemnessandprogressionofgastriccancerbyinteractingwithhsp90toactivatenotch1signalingpathway
AT wenguangyuan circslc4a7acceleratesstemnessandprogressionofgastriccancerbyinteractingwithhsp90toactivatenotch1signalingpathway
AT weishang circslc4a7acceleratesstemnessandprogressionofgastriccancerbyinteractingwithhsp90toactivatenotch1signalingpathway
AT haoranwang circslc4a7acceleratesstemnessandprogressionofgastriccancerbyinteractingwithhsp90toactivatenotch1signalingpathway
AT shangleining circslc4a7acceleratesstemnessandprogressionofgastriccancerbyinteractingwithhsp90toactivatenotch1signalingpathway
AT juliu circslc4a7acceleratesstemnessandprogressionofgastriccancerbyinteractingwithhsp90toactivatenotch1signalingpathway