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Reducing the inherent auto-inhibitory interaction within the pegRNA enhances prime editing efficiency

Prime editing systems have enabled the incorporation of precise edits within a genome without introducing double strand breaks. Previous studies defined an optimal primer binding site (PBS) length for the pegRNA of ∼13 nucleotides depending on the sequence composition. However, optimal PBS length ch...

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Detalles Bibliográficos
Autores principales:	Ponnienselvan, Karthikeyan, Liu, Pengpeng, Nyalile, Thomas, Oikemus, Sarah, Maitland, Stacy A, Lawson, Nathan D, Luban, Jeremy, Wolfe, Scot A
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2023
Materias:	RNA and RNA-protein complexes
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10359601/ https://www.ncbi.nlm.nih.gov/pubmed/37246708 http://dx.doi.org/10.1093/nar/gkad456

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